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In-vitro lung mechanical perfusate, preparation method and application thereof

A perfusate and lung technology, applied in the field of isolated lung mechanical perfusate and its preparation, can solve the problems of high price, difficulty in effective treatment, single product, etc.

Inactive Publication Date: 2021-03-02
WUXI PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the perfusion preservation solution used in lung transplantation is mainly Steen solution, which is expensive and has a single product, and the use of Steen solution to mechanically perfuse the lung can only last for 12 hours at most
This limits the function of EVLP for perfusion repair, such as for bacterial pneumonia, it is difficult to effectively treat within 12 hours

Method used

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  • In-vitro lung mechanical perfusate, preparation method and application thereof
  • In-vitro lung mechanical perfusate, preparation method and application thereof
  • In-vitro lung mechanical perfusate, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Dissolve human serum albumin, polysucrose 70, dextran 40, mannitol, and nitroglycerin in DMEM / F12 (1:1) medium according to the concentrations in the table below, and then adjust the pH to 7.4 with sodium hydroxide.

[0035] components Concentration(g / L) human serum albumin 10 Ficoll 70 60 Dextran 40 10 Mannitol 7.2 Nitroglycerin 10

[0036] The isolated lung mechanical perfusion fluid prepared in Example 1 was used for animal experiments.

[0037] Anesthesia and weighing: Intramuscularly inject 2ml of atropine to the pigs used in the experiment, and then inject 2ml of Sotapine 15 minutes later. After the pigs are stabilized, they are weighed, and the weight is 30.0kg. An intravenous line was established and propofol was administered continuously intravenously.

[0038] Chest opening: endotracheal intubation, after breathing support, the chest cavity is opened.

[0039] In vivo perfusion of the lung: 10×12 special-shaped jo...

Embodiment 2

[0047] Dissolve human serum albumin, polysucrose 70, dextran 40, mannitol, and nitroglycerin in DMEM / F12 (1:1) medium according to the concentrations in the table below, and then adjust the pH to 7.4 with sodium hydroxide.

[0048]

[0049]

[0050] The isolated lung mechanical perfusion solution prepared in Example 2 was used to repair the isolated lung perfusion, which can be maintained for 8 hours without edema in the lung.

Embodiment 3

[0052] Dissolve human serum albumin, polysucrose 70, dextran 40, mannitol, and nitroglycerin in DMEM / F12 (1:1) medium according to the concentrations in the table below, and then adjust the pH to 7.4 with sodium hydroxide.

[0053] components Concentration(g / L) human serum albumin 8 Ficoll 70 65 Dextran 40 15 Mannitol 10 Nitroglycerin 15

[0054] The isolated lung mechanical perfusion solution prepared in Example 3 was used to repair the isolated lung perfusion, which can be maintained for 18 hours without edema in the lung.

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Abstract

The invention relates to in-vitro lung mechanical perfusate, a preparation method and application thereof. The in-vitro lung mechanical perfusate comprises a basic medium, human serum albumin, polysucrose, dextran, mannitol and nitroglycerin; in the in-vitro lung mechanical perfusate, the concentration of the human serum albumin is 5g / L-20g / L, the concentration of the polysucrose is 25g / L-70g / L, the concentration of the dextran is 1g / L-50g / L, the concentration of the mannitol is 1g / L-15g / L, and the concentration of the nitroglycerin is 1g / L-20g / L. The in-vitro lung mechanical perfusate can keep the in-vitro lung function stable for a long time, relieve pulmonary edema, repair the lung, increase the utilization rate of the donor lung, reduce the use amount of albumin and greatly reduce thecost.

Description

technical field [0001] The invention relates to the technical field of organ preservation, in particular to a mechanical perfusion solution for isolated lungs and its preparation method and application. Background technique [0002] Since the pioneering of lung transplantation technology (Lung Transplantations), clinical lung transplantation has achieved considerable development and great success in the past few decades. Lung transplantation is an effective way to treat end-stage pulmonary parenchymal disease and pulmonary vascular disease, and the number of lung transplantation is still increasing rapidly every year. Lung transplantation is the ultimate solution for patients with end-stage respiratory failure, yet the rate of lung organs obtained from deceased donors is much lower than other solid organs. The low proportion of lungs available for transplantation is due to multiple mechanisms of injury to the donor lungs during brain death, organ donation (eg, ventilator-ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 陈静瑜王瑞彬卫栋袁景泉刘峰卢艳杨振坤林祥华王伟唐均匀毛禹康
Owner WUXI PEOPLES HOSPITAL
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