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Rhein-loaded lactoferrin nanoparticle and preparation method and application thereof

A lactoferrin and nanoparticle technology, applied in the field of rhein-loaded lactoferrin nanoparticles and their preparation, can solve the problems of poor colon targeting ability, low water solubility, low bioavailability and the like, and achieve biocompatibility Good properties, simple preparation method and good stability

Active Publication Date: 2021-03-12
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, there are some problems when rhein (RH) is used, such as: low water solubility, low bioavailability, poor colon targeting ability

Method used

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  • Rhein-loaded lactoferrin nanoparticle and preparation method and application thereof
  • Rhein-loaded lactoferrin nanoparticle and preparation method and application thereof
  • Rhein-loaded lactoferrin nanoparticle and preparation method and application thereof

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Embodiment 1

[0070] Embodiment 1, preparation of lactoferrin rhein nanoparticles of the present invention

[0071]Lactoferrin rhein nanoparticles (lactoferrin nanoparticles loaded with rhein) were prepared by dialysis technology. Lactoferrin (LF) 50 mg was dissolved in 20 mL of deionized water to prepare an LF solution. At the same time, 5 mg of rhein (RH) was completely dissolved in 1 mL of DMSO to obtain a RH solution. Under constant magnetic stirring, the above RH solution was added dropwise into the LF solution. The mixture was then placed in an ice bath and sonicated using a probe sonicator (Sonicator XL, Misonix, Melville, NY, USA) at 50% amplitude power for 5 minutes to form an emulsion. Then, the emulsion was dialyzed against deionized water (molecular weight cut-off 1000 Da) for 6 h to remove DMSO. Subsequently, it was filtered with a 0.45 μm filter to remove dust and free drug, and RH-NPs were obtained after filtration.

[0072] Afterwards, 20 mg of hyaluronic acid (HA) was a...

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Abstract

The invention provides a rhein-loaded lactoferrin nanoparticle and a preparation method and application thereof, and belongs to the technical field of biomedical materials. Specifically, the inventionprovides a drug-loaded lactoferrin nanoparticle which is a nanoparticle with a three-layer structure, the three-layer structure comprises a pectin layer, a hyaluronic acid layer and a medicine-carrying lactoferrin layer from outside to inside. The medicine is preferably rhein. The drug-loaded dual-targeting drug delivery nanoparticle provided by the invention has good biocompatibility and good stability, can be orally taken, and has good targeting ability. The nanoparticle loaded with rheinic acid has good colon lesion inflammation and colon injury targeting ability. The nanoparticle maintains stability in stomach and small intestine during oral administration, slowly releases drugs, continuously releases drugs after targeting colon lesion sites, and has good treatment effect on colitis,especially ulcerative colitis. The nanoparticle is excellent in treatment effect and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a lactoferrin nanoparticle loaded with rhein and its preparation method and application. Background technique [0002] Ulcerative colitis (UC), a common inflammatory disease of the colon, is increasing in incidence and prevalence worldwide and is more prevalent among young adults, suggesting that it is a global disease. UC is characterized by disruption of the epithelial barrier and an imbalance in inflammatory homeostasis. Therefore, the main goals of clinical UC treatment are to accelerate colonic healing and reduce inflammation. Currently, the first-line therapy for UC mainly focuses on anti-inflammatory drugs, immunomodulators and biological products. However, adverse reactions, such as nephrotoxicity, neurotoxicity, and opportunistic infections, have been reported in patients using various clinically approved drugs. Screening natural bioactive com...

Claims

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Application Information

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IPC IPC(8): A61K9/52A61K31/192A61K47/36A61K47/42A61P1/04A61P29/00
CPCA61K9/5169A61K9/5161A61K31/192A61P1/04A61P29/00Y02A50/30
Inventor 高飞罗锐锋林美斯章津铭廖婉石金凤张晨陈其艳
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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