Mirror image protein information storage and coding technology
An information storage and mirroring technology, applied in the field of bioinformatics, can solve the problems of difficult DNA chemical preparation, inability to realize information preservation, poor biological stability, etc., and achieve simple and convenient information writing, high tolerance to storage conditions, and easy access Effect
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Embodiment 1
[0039] Mirror protein fragment preparation and sequencing
[0040] 1.1. Preparation of mirror image protein fragments (coding information writing)
[0041] Using the Fmoc solid-phase peptide synthesis method, prepare the following mirror image peptide fragments, refer to figure 1 (a):
[0042] A1 mirror image peptide fragment:
[0043] h 2 N-fGhvwekapGshhwGk(Biotin)-CONH 2 (wherein, k(Biotin) means biotinylated lysine). The specific operation steps are as follows:
[0044] First, add Rink Amide-AM resin to the peptide synthesis tube, use dimethylformamide (DMF) / dichloromethane (DCM) (volume ratio 1:1) to swell, and add benzotriazole-N, N,N',N'-Tetramethyluronium hexafluorophosphate (HBTU, 3.8 equivalents) / Hydroxybenzotriazole (HOBt, 4 equivalents) / Diisopropylethylamine (DIEA, 8 equivalents ) / Fmoc-protected target peptide C-terminal first amino acid (4 times equivalent) was dissolved in DMF, added to the peptide synthesis tube containing resin to react for 2 hours, the r...
Embodiment 2
[0061] Mirror protein encoding library for storing and encoding Chinese characters, see the operation process Figure 7 and 8 .
[0062] 2.1, "Chinese" hexadecimal encoding rules based on mirror protein (information encoding)
[0063] Using the mirror protein coding system, based on the hexadecimal national standard code, it is used for information storage and coding of Chinese words. As shown in Table 1, taking "Tsinghua University", "China University of Science and Technology", "Shanghai University" and "mirror protein" as examples, the present invention defines a mirror protein coding rule, as shown in Table 1, All characters in hexadecimal corresponding to unique d-amino acid coding sequences are listed. This artificially designed encoding rule can further increase the privacy and security of the encoded information. According to the rules in Table 1, the mirror protein coding sequences corresponding to the obtained Chinese information such as "Tsinghua University", "C...
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