Multifunctional agrobacterium strain and application thereof

A multifunctional technology of Agrobacterium, applied in the field of microorganisms, can solve the problem of host seed germination, host initial root development and seedling growth without description, hindering the use range of Agrobacterium, lack of Agrobacterium function, exploring nitrogen fixation function, promoting seed germination function, etc. problem, to achieve the effect of promoting seed germination rate and seedling development, promoting seed germination, and promoting seedling growth

Active Publication Date: 2021-03-19
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention of Agrobacterium inoculum in the above patent documents has the following defects and deficiencies: (1) It only focuses on the symbiotic relationship between the selected Agrobacterium and the growth and development of the crop host in the middle and late stages, and has a negative effect on the germination of the host seed and the initial root system of the host. Development and seedling growth are not described; (2) The lack of exploration of other functions of Agrobacterium itself (such as nitrogen fixation, seed germination promotion, and adversity growth, etc.), hinders the use of Agrobacterium in the agricultural production process

Method used

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  • Multifunctional agrobacterium strain and application thereof
  • Multifunctional agrobacterium strain and application thereof
  • Multifunctional agrobacterium strain and application thereof

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Embodiment Construction

[0026] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. On the contrary, these embodiments are provided to make the understanding of the present disclosure more thorough and comprehensive.

[0027] 1. Screening of strains

[0028] 1. Soil sample collection

[0029] The five-point sampling method was used to collect wheat-corn saline-alkali farmland soil with a planting period of 15 years in the Yellow River Delta, mixed it into a sterilized sealed bag, pasted the sampling site information label, and transported the soil sample to the laboratory at low temperature.

[0030] 2. Preparation of selective mediu...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to multifunctional agrobacterium and application thereof. The multifunctional agrobacterium strain is an agrobacterium sp.SD307 strain, is preserved in the China Center for Type Culture Collection (CCTCC) in November 12, 2020, and has a preservation number of CCTCC M2020730. The agrobacterium sp. SD307 strainprovided by the invention not only can promote seed germination and seedling growth of crops, but also has multiple functions of nitrogen fixation and heavy metal resistance.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a multifunctional agrobacterium and its application. Background technique [0002] At present, in the process of agricultural production, although the use of chemical fertilizers can significantly increase the yield of crops, in order to meet the increasing demand for agricultural products, farmers use more and more chemical fertilizers, resulting in unreasonable use of chemical fertilizers . This long-term agricultural production behavior of excessive use of chemical fertilizers has led to the continuous increase of salinity in the soil, especially the serious problem of nitrate accumulation, which has caused a series of soil and environmental problems, such as salinization of the soil surface, soil organic matter content decline, soil structure damage, etc. These problems have seriously affected the germination of crop seeds and the growth of seedlings, res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00B09C1/10C12R1/01
CPCC12N1/20A01N63/20B09C1/10B09C2101/00
Inventor 戴九兰何欢张律晴白利勇
Owner SHANDONG UNIV
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