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Preparation method of icariin

A technology of icariin and epimedium, which is applied in the field of icariin preparation, can solve the problems of destruction of active ingredients, reduction of the extraction amount of active ingredients and the like, and achieves the effect of good extraction effect and high content

Pending Publication Date: 2021-03-26
ZHENGZHOU FURUITANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the heating and reflux method needs repeated heating and multiple reflux; the concentration process in the water extraction and alcohol precipitation method is too long, and the active ingredients will be destroyed; reduce

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The active ingredient icariin in Epimedium is extracted by percolation method, and the process includes the following steps:

[0021] (1) Infiltration and impregnation: Weigh 800g of washed Epimedium macrophyllum and crush it into a coarse powder, pass through a 40-mesh sieve, add double the amount of 75% ethanol solution for infiltration, and the infiltration time is 2 hours. into the percolation device, and add twice the amount of 75% ethanol solution for impregnation, and the impregnation time is 24 hours;

[0022] (2) percolation: adopt 75% ethanol to carry out percolation after impregnation, percolation flow rate 5ml / min / kg;

[0023] (3) Collection: collect the percolate, concentrate under reduced pressure to obtain crude icariin.

[0024] (4) Purification: The crude product of icariin is purified by a column using a macroporous resin. After the purification is completed, gradient elution is carried out with 35%, 45%, 55%, 65%, 75%, and 85% ethanol successively. ...

Embodiment 2

[0028] The active ingredient icariin in Epimedium is extracted by percolation method, and the process includes the following steps:

[0029] (1) Infiltration and impregnation: Weigh 1000g of washed Epimedium grandiflora and crush it into a coarse powder, pass through a 40-mesh sieve, add double the amount of 55% ethanol solution for infiltration, and the infiltration time is 3 hours. into the percolation device, and add twice the amount of 55% ethanol solution for impregnation, and the impregnation time is 30 hours;

[0030] (2) Percolation: use 50% ethanol for percolation after impregnation. Percolation flow rate 4ml / min / kg;

[0031] (3) Collection: collect the percolate, concentrate under reduced pressure to obtain crude icariin.

[0032] (4) Purification: The crude product of icariin is purified by a column using a macroporous resin. After the purification is completed, gradient elution is carried out with 30%, 40%, 50%, 60%, 70%, and 90% ethanol successively, and the elu...

Embodiment 3

[0036] The active ingredient icariin in Epimedium is extracted by percolation method, and the process includes the following steps:

[0037] (1) Infiltration and impregnation: Weigh 800g of washed Epimedium cordifolia and crush it into a coarse powder, pass through a 40-mesh sieve, add double the amount of 65% ethanol solution for infiltration, and the infiltration time is 2 hours. into the percolation device, and add twice the amount of 65% ethanol solution for impregnation, and the impregnation time is 40 hours;

[0038] (2) percolation: adopt 80% ethanol to carry out percolation after impregnation, percolation flow rate 4ml / min / kg;

[0039] (3) Collection: collect the percolate, concentrate under reduced pressure to obtain crude icariin.

[0040] (4) Purification: The crude product of icariin is purified by column with macroporous resin, and after the purification is completed, gradient elution is performed with 30%, 50%, 70%, and 90% ethanol in sequence, and the eluent 1 ...

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PUM

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Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction and preparation, and particularly relates to a preparation method of icariin. The preparation method of the icariin comprises the following steps of crushing cleanly-washed herba epimedii into coarse powder; adding one-time amount of ethanol solution with the concentration of 40 percent to 90 percent for infiltration for 1 to 5 hours; filling infiltration liquid into a percolation device; adding two-time amount of ethanol solution with the concentration of 40 percent to 80 percent for impregnation for 18 to50 hours; then, performing percolation after impregnation at the percolation flow rate of 3 to 8 ml / min / kg; collecting percolates; performing reduced pressure concentration to obtain an icariin crudeproduct; performing column purification on the icariin crude product by using macroporous resin; performing gradient elution after the purification is finished; collecting first eluent; performing chromatography on the first eluent by using a gel column; then performing gradient elution; collecting second eluent; performing reduced pressure concentration on the second eluent; and performing drying and crushing to obtain the icariin. The preparation method of the icariin provided by the invention has the advantages of good extraction effect and high icariin conversion rate.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of traditional Chinese medicines, and in particular relates to a method for preparing icariin. Background technique [0002] The source of Epimedium is a variety of plants of the genus Epimedium in the family Berberaceae. It is a commonly used traditional Chinese medicine in China, also known as Xianlingpi. It tastes pungent, sweet, and warm in nature. Value, the traditional Chinese medicine Epimedium in my country has the functions of nourishing kidney yang, dispelling rheumatism, and strengthening bones and muscles. Epimedium contains a variety of ingredients, total flavonoids and icariin have a variety of biological activities, which are the main active ingredients, and also contain alkaloids, lignans, polysaccharides and trace elements, phenolic glycosides, organic acids, violet Ketones and phenylethanol glycosides, etc. Modern pharmacological studies have proved that Epime...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H17/07
CPCC07H1/08C07H17/07
Inventor 罗会军
Owner ZHENGZHOU FURUITANG PHARMA