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Application of wheat TaTFIIB gene in regulation and control of wheat plant height development

A wheat and high school technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of unclear biological functions and molecular mechanisms, and achieve the goals of improving and improving germplasm resources, reducing plant height, and reducing plant height Effect

Active Publication Date: 2021-03-30
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, reports on the structure and function of the TFIIB gene in wheat are still poorly understood, and the biological function and molecular mechanism of the gene in wheat are still unclear

Method used

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  • Application of wheat TaTFIIB gene in regulation and control of wheat plant height development
  • Application of wheat TaTFIIB gene in regulation and control of wheat plant height development
  • Application of wheat TaTFIIB gene in regulation and control of wheat plant height development

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Genotyping of TaTFIIB-B gene in core germplasm material

[0040] 1. Select wheat micro-core germplasm materials (105 cultivars and 157 landraces) distributed in different wheat regions in China as the excavation materials for polymorphic SNP loci.

[0041] 2. Exon capture to obtain SNPs of micro-core germplasm material

[0042]The DNA of 262 materials was constructed and sequenced using Roche Exon Capture Kit (SeqCap Pure Capture Bead Kit, 06977952001, Roche). For specific methods, refer to the kit instructions.

[0043] 3. Use BWA software for whole genome sequence alignment, use GATK software for variant detection and genotyping, and obtain the genotype of TaTFIIB-B gene in 262 materials.

Embodiment 2

[0044] Example 2 Association analysis between SNP loci of TaTFIIB-B gene and plant height traits

[0045] Using the plant height phenotype data of 262 micro-core germplasm materials for three years and three points (in Luoyang, Henan in 2002, in Luoyang in Henan in 2005, and in Beijing in 2006), an association analysis was carried out, and it was found that the TaTFIIB-B gene was associated with plant height traits. There is a significant correlation, and the results are shown in Table 1.

[0046] Table 1 Significantly associated SNP sites with plant height

[0047]

Embodiment 3

[0048] Example 3 Sequence of wheat TaTFIIB-B gene

[0049] According to TraesCS5B02G324000 gene number, compare on the wheat genome database website to obtain its homologous gene numbers TraesCS5A02G323500 and TraesCS5D02G330500, and obtain the gene sequences of TaTFIIB gene on homologous chromosomes A, B and D, respectively as shown in SEQ ID NO.4-6 showed that the gene was located on the fifth chromosome homologous group.

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Abstract

The invention relates to the technical field of plant molecular biology, in particular to application of a wheat TaTFIIB gene in regulation and control of wheat plant height development. The inventionprovides application of wheat TaTFIIB protein, a coding gene of the wheat TaTFIIB protein or an inhibition factor of the coding gene of the wheat TaTFIIB protein in regulation and control of plant height. It is found that the wheat TaTFIIB gene can negatively regulate and control the plant height, and the plant height can be effectively reduced by reducing the expression quantity of the TaTFIIB gene. The disclosure of new functions of the TaTFIIB gene provides new gene targets and resources for plant height regulation and control, wherein the wheat TaTFIIB gene can be widely applied to the fields of wheat genetic breeding, germplasm resource improvement and TaTFIIB gene plant cultivation and editing, and plays an important role in improving germplasm resources of crops such as wheat.

Description

technical field [0001] The invention relates to the technical field of plant molecular biology, in particular to the application of wheat TaTFIIB gene in regulating wheat plant height development. Background technique [0002] Wheat is an important food crop in the world, and it is also the staple food for more than 1 / 3 of the world's population. In recent years, China has become the world's largest wheat producer and consumer, so the output of wheat is closely related to the country's food security. With the increase of population, the reduction of arable land and the increase of production cost, how to increase wheat output to ensure food security has become an urgent problem to be solved. In the 1970s, the Green Revolution led by the semi-dwarf gene of wheat brought a great increase in global wheat yield, indicating that wheat plant height has an important influence on the formation of yield. Understanding the mechanism of yield formation is of great significance for us...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 毛龙付明雪李爱丽耿帅锋
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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