A nanopore single-molecule protein sequencer

Abstract

The invention provides a nanopore single-molecule protein sequencer, which mainly includes three parts: a nanopore array chip system, a microcurrent detection system, and a data processing and construction system; the chip mainly includes an amino acid electrical screening chip and a specific nanopore-based The amino acid sequence reading chip, etc., the design principle is to design a series of biological nanopores with targeted recognition of amino acids according to the properties of amino acids such as hydrophilicity, hydrophobicity, polarity, and chargeability, and obtain the nanopore characteristics of the amino acids that make up the protein to be tested one by one. Ion current signal; through the arrayed microcurrent measurement system, the protein sequence characteristic information in each nanopore can be obtained; furthermore, the amino acid sequence identification, correction, integration and reading can be realized by using the standard nanopore model peptide sequence information library. The ultra-high resolution of a single amino acid meets the technical requirements of protein sequencing.

Description

technical field [0001] The present invention relates to the fields of biotechnology and medicine, in particular to a multi-channel nanopore sequencing device, which can perform direct, efficient and accurate analysis and detection of protein amino acid sequences, and can be flexibly extended to micro-protein or unknown protein sequencing in biological samples Analytical equipment. Background technique [0002] Protein maintains various life processes of cells and is one of the most important components of living organisms. The precise determination of the amino acid sequence of proteins in organisms is the basis for understanding the biological functions of proteins. The current protein sequencing methods are mainly Edman degradation sequencing and mass spectrometry. The Edman method is mainly used to analyze the N-terminus of proteins. The mass spectrometry method is based on the regular fragmentation of peptide molecules after protein digestion in mass spectrometry, looki...

AI Technical Summary

Problems solved by technology

The current nanopore device can preliminarily realize the resolution of several simple amino acids, but it is still unable to meet the effective identification of 20 kinds of amino acids and their post-translational modifications, and it is difficult to obtain amino acid sequence information

[0005] The main challenges for the development of nanopore single-molecule protein sequencing devices are: (1) It is urgent to further develop highly sensitive and specific nanopore chips to achieve single-molecule resolution of different amino acids, and build a multi-channel detection system to achieve direct sequencing of unknown proteins; ( 2) The current nanopore data acquisition device is still unable to realize the extremely small current signal (

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