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Analysis method for diversity of acute lung injury intestinal flora

A technology for acute lung injury and intestinal flora, applied in sequence analysis, biochemical equipment and methods, and microbial determination/inspection, etc. and other problems to achieve the effect of improving the effect of treatment and improving the success rate of treatment

Pending Publication Date: 2021-03-30
李志军
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Problems solved by technology

[0005] In view of the deficiencies in the prior art, the present invention provides an analysis method for the diversity of intestinal flora in acute lung injury, which solves the problem that the analysis of the diversity of intestinal flora in the above background technology is not targeted and easy Data errors are generated, which leads to a decrease in the accuracy of the judgment of patients with acute lung injury

Method used

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Embodiment Construction

[0023] In the following, the technical solutions in the embodiments of the present invention are clearly and completely described. Apparently, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0024] The present invention provides a technical solution: a method for analyzing the diversity of intestinal flora in acute lung injury, comprising the following steps:

[0025] S1. Collect samples: excrete the feces of patients with acute lung injury into a clean container, and stick a small amount with a cotton swab and put it into a test tube containing sterile saline, cover and seal it, put the test tube into a medical freezer, and refrigerate 24 hours, so that the flora is stable and does not change;

[0026] S2, sample DNA extraction: deliver the test tube in step S1, collecting the sample to the sample extraction place, and extract the DNA template;

[0027] S3, PCR amplification: extraction step S2, bacter...

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Abstract

The invention discloses an analysis method for the diversity of acute lung injury intestinal flora, relates to the technical field of acute lung injury intestinal flora diversity analysis, and particularly relates to an analysis method for the diversity of the acute lung injury intestinal flora. The analysis method which comprises the steps of S1, collecting a sample; S2, extracting DNA of the sample DNA; S3, carrying out PCR amplification; and S4, carrying out data information analysis, specifically, S401, conducting effective sequence statistics; S402, performing effective sequence length distribution statistics; S403, carrying out OUT clustering and species information annotation; S404, obtaining a diversity index; and S405, carrying out metagenome sequencing analysis. According to themethod for analyzing the diversity of the acute lung injury intestinal flora, detailed specific values of a patient can be effectively obtained according to the diversity of the acute lung injury intestinal flora and compared with standard data values, the detailed situation of the lung of the patient can be effectively judged, and a detailed table can be issued for filing. so that targeted treatment can be effectively carried out according to the lung condition of the patient.

Description

technical field [0001] The invention relates to the technical field of analyzing the diversity of intestinal flora in acute lung injury, in particular to a method for analyzing the diversity of intestinal flora in acute lung injury. Background technique [0002] Acute lung injury is the injury of alveolar epithelial cells and capillary endothelial cells caused by various direct and indirect injury factors, resulting in diffuse pulmonary interstitial and alveolar edema, resulting in acute hypoxic respiratory insufficiency. The pathophysiological features are reduced lung volume, decreased lung compliance, and imbalance of ventilation / perfusion flow ratio, clinically manifested as progressive hypoxemia and respiratory distress, and lung imaging manifested as heterogeneous exudative lesions , and its development to a severe stage (oxygenation index <200) is called acute respiratory distress syndrome. [0003] There are 100 trillion microorganisms such as bacteria, fungi, vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B40/10G16B25/20G16B30/00G16B20/00C12Q1/689C12Q1/686C12Q1/06
CPCC12Q1/686C12Q1/689G16B20/00G16B25/20G16B30/00G16B40/10C12Q2565/125
Inventor 李志军
Owner 李志军
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