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A strain of Lactobacillus rumen that can protect the intestinal barrier and its application

A technology of Lactobacillus stomach and intestinal barrier, applied in the field of microorganisms, can solve the problems affecting the success of treatment, etc., achieve the effect of reducing damage, broad market prospects, and reducing disease activity index

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of drugs related to intestinal inflammation (inhibitors, hormones, antibiotics) may lead to hypertension, diabetes, osteoporosis and other diseases, thereby affecting the success of treatment

Method used

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  • A strain of Lactobacillus rumen that can protect the intestinal barrier and its application
  • A strain of Lactobacillus rumen that can protect the intestinal barrier and its application
  • A strain of Lactobacillus rumen that can protect the intestinal barrier and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Screening, identification, cultivation, observation and preservation of Lactobacillus rumen CCFM1140

[0075] 1. Screening

[0076] Take 1g of feces samples from healthy people in Wusu, Xinjiang, spread them on MRS solid medium after gradient dilution, and culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; The white and yellowish colonies were streaked on the MRS solid medium, and purified and cultivated under anaerobic conditions at 37°C. This operation was repeated 3 times to obtain a purified single colony; pick a single colony and place it on the MRS solid medium Mark the upper line, culture anaerobically at 37°C for 36 hours, and perform Gram staining on the obtained colonies (for the Gram staining method, refer to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the morphology of the colonies, and according to the textbook "Common Bacterial System Identification Manual" (Auth...

Embodiment 2

[0091] Embodiment 2: Preparation of Lactobacillus rumen CCFM1140 bacterial suspension

[0092] (1) Dip the bacterial solution of Lactobacillus rumen CCFM1140 from the glycerol tube, streak it on the MRS solid medium, culture it at 37°C for 48 hours under anaerobic environment, and obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, In an anaerobic environment, culture at 37° C. for 48 hours for activation culture, and repeat this operation 3 times to obtain the activated bacterial liquid.

[0093] (2) Inoculate the activated bacterial liquid obtained in step (1) into the MRS liquid medium according to the inoculum size of 2% (v / v), cultivate at 37°C for 24 hours to obtain a fermentation liquid, and collect the bacteria by centrifugation of the fermentation liquid body, resuspend the cell body with physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made into bacterial suspension.

Embodiment 3

[0094] Example 3: Relief effect of Lactobacillus rumen CCFM1140 on DSS-induced intestinal barrier damage in mice

[0095] The modeling steps are as follows:

[0096] (1) Preparation of 2.5% dextran sodium sulfate (DSS) solution: the DSS was made into a 2.5% (w / v) DSS solution with sterilized tap water.

[0097] (2) 24 8-week-old healthy male C57BL / 6J mice were randomly divided into 3 groups, named as control group (Control), modeling group (DSS), Lactobacillus rumen CCFM1140 intervention group (CCFM1140 +DSS); 8 mice were used in each group, and the experimental scheme and the treatment methods of mice in each group were shown in Table 4.

[0098] (3) Treat the control group (Control), the modeling group (DSS), and the Lactobacillus rumen CCFM1140 intervention group;

[0099] Among them, the treatment method of the Lactobacillus rumen CCFM1140 intervention group is as follows: on the first 7 days of the experiment, 5 × 10 9 CFU / mL Lactobacillus rumenensis suspension 200μL, ...

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Abstract

The invention discloses a rumen lactobacillus capable of protecting the intestinal barrier and its application, belonging to the field of biotechnology. The invention provides a strain of Lactobacillus rumen, which can significantly reduce the disease activity index of mice with intestinal damage induced by DSS. Compared with the mice in the model group, the tissue damage of the mice in the Lactobacillus rumen CCFM1140 intervention group is significantly reduced, and the inflammation is promoted. The concentrations of the cytokines TNF‑α, IL‑1β, and IL‑17 were decreased, and the concentration of the anti-inflammatory cytokine IL‑10 was upregulated. The strain has the function of improving intestinal mechanical barrier, immune barrier and biological barrier, can be used as the main component of probiotic preparations, and has broad market prospects.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rumen capable of protecting the intestinal barrier and its application, in particular to a bacterial strain capable of protecting the intestinal barrier, which can be added to various health foods and health foods, and belongs to the technical field of microorganisms. Background technique [0002] Intestinal barrier refers to the sum of structures and functions that can prevent pathogenic bacteria and toxins in the intestinal lumen from invading the blood system and other tissues and organs through the intestinal mucosa, and plays an important role in human health. The intestinal barrier is composed of mechanical barrier, immune barrier, biological barrier and chemical barrier. The mechanical barrier refers to the mucus secreted by the intestinal epithelium, intestinal epithelial cells and their tight junctions, etc., which are directly related to the occurrence of intestinal diseases; the immune barri...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A61K35/747A61P1/00A23L33/135A23L2/38A23L2/84C12R1/225
CPCC12N1/20C12N1/04A61K35/747A61P1/00A23L33/135A23L2/382A23L2/84A23V2002/00A23V2400/177A23V2200/3204
Inventor 陈卫杨波王烁崔树茂陆文伟赵建新张灏
Owner JIANGNAN UNIV
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