Magnetic fluid IgM-IgG antibody detection device and detection method thereof

Abstract

The invention provides a magnetic fluid IgM-IgG antibody detection device and a detection method thereof. The detection method comprises the following steps of: introducing a magnetic fluid detection liquid, filling a preset flow channel with the magnetic fluid detection liquid, introducing a to-be-detected sample, intermittently starting a magnetic mixing and stirring electromagnet, and keeping the magnetic mixing and stirring electromagnet open all the time; after preset time, closing the magnetic separation waste liquid outlet and the magnetic fluid detection liquid inlet, opening the waste liquid outlet, and closing the magnetic mixing and stirring electromagnet and the magnetic separation and stirring electromagnet; and observing whether the mixed fluid in the detection boss flow channel emits fluorescence or not through the detection camera to judge whether a target antibody exists in the to-be-detected sample or not. According to the magnetic fluid IgM-IgG antibody detection device, a to-be-detected sample and a magnetofluid fluorescent marker mixed solution are fully subjected to immunoreaction on the detection chip, so that the sensitivity, specificity and detection efficiency of sample antibody detection are improved, meanwhile, after chip detection is completed, a new detection chip can be directly pushed into the chip to replace the detected detection chip, and rapid large-scale detection is realized.

Description

technical field [0001] The invention relates to the technical field of fluorescence immunoassay, in particular to a magnetic fluid IgM-IgG antibody detection device and a detection method thereof. Background technique [0002] Fluorescence immunoassay technology is a new high-precision and low-cost immunoassay assay technology after radioimmunoassay technology and enzyme immunoassay technology. Fluorescence immunoassay technology is mainly composed of immunoassay and fluorescence analysis. The microfluidic fluorescent immune chip was established in the capillary microfluidic system in the 1990s. With the rapid development of microfluidic chip technology, a set of small sample volume, easy operation, batch processing and automatic antibody detection can be formed. Emerging IgM-IgG antibody detection technology. Although the technology is still in the initial stage of laboratory development, the relevant testing equipment market is mainly controlled by overseas markets, and ...

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a magnetic fluid IgM-IgG antibody detection device and a detection method thereof, aiming at solving the problems of low detection sensitivity and poor specificity of the existing fluorescent immunoassay technology

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