A preparation method and application method of ultrasound-targeted microbubbles carrying simvastatin

Abstract

The invention relates to a preparation method of ultrasound-targeted microbubbles carrying simvastatin, which comprises dissolving simvastatin in tetrahydrofuran and diluting physiological saline to a concentration of 0.01-0.1 mg / mL; adding trimethyl chitosan, Sodium tripolyphosphate, then, compounded into a stable space network, obtained a suspension, freeze-dried and redissolved, transferred the obtained liquid to a vial filled with C3F8 gas, and vibrated the vial with a mechanical oscillator to form a 0.05 mg / mL simvastatin microbubbles, fill the remaining space of the vial with high-pressure C3F8 gas and seal it up, and refrigerate it for later use; inject simvastatin microbubbles into the knee joint cavity and apply ultrasound at the same time, control the ultrasound intensity and time, and target to induce microbubble rupture to reach the maximum ratio. The administration is highly targeted, the medication cycle is shortened, the side effects of long-term medication are reduced, and the dosage of the medication is reduced.

Description

technical field

[0001] The invention belongs to the field of new drug dosage forms and applications thereof, and in particular relates to an ultrasound-targeted microbubble carrying simvastatin and its application for treating osteoarthritis. Background technique

[0002] Arthritis (osteoarthritis, OA) is a common clinical chronic, articular cartilage degenerative disease, the main symptoms are joint pain, stiffness and dysfunction. In my country, OA mainly occurs in middle-aged and elderly people, and the incidence rate increases with age. Epidemiological surveys show that 90% of women and 80% of men over 65 years old suffer from OA, and the prevalence of knee OA exceeds 10%. %, is the leading cause of physical disability in the elderly population. With the increase of sports trauma and life expectancy, the incidence of OA is increasing year by year, and the average age of OA tends to be younger. With the progress of OA-related epidemiology and molecular biology research, ...

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