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Application of BIIB021 in preparation of medicine for preventing and/or treating adenovirus infection

A 1. BIIB021, adenovirus technology, applied in the field of medicine, can solve problems such as undiscovered, achieve the effect of inhibiting replication, wide application prospects, and short drug cycle

Pending Publication Date: 2022-06-24
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the treatment of adenovirus infection with BIIB021

Method used

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  • Application of BIIB021 in preparation of medicine for preventing and/or treating adenovirus infection
  • Application of BIIB021 in preparation of medicine for preventing and/or treating adenovirus infection
  • Application of BIIB021 in preparation of medicine for preventing and/or treating adenovirus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Evaluation of Cytotoxicity of BIIB021 in Vero Cell Line

[0058] 1 cell culture

[0059] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / ml, streptomycin 100μg / ml) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / ml, passage density not higher than 5×10 4 cells / ml.

[0060] 2 Drug-treated cells

[0061] Vero cells by 1 x 10 4 Cells / well (volume 100 μL) were seeded in 96-well cell culture plates, and cultured for 24 hours until the confluence of cell wells reached 80%; 200 μL of medium (DMEM medium + 2% serum + double antibody) was used in each well to prepare drugs, and Add to the corresponding cell wells and mix. Seven concentration gradients were set for the drug, and two duplicate wells were set for each gradient concentration, and the final concentrations were 0.007 μM, 0.02 μM, 0.06 μM, 0.19 μM, 0.56 μM, 1.67 μM and 5 μM,...

Embodiment 2

[0066] Example 2: Evaluation of anti-AdV3 adenovirus activity of BIIB021 in Vero cell line

[0067] 1 cell culture

[0068] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / ml, streptomycin 100μg / ml) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / ml, passage density not higher than 5×10 4 cells / ml.

[0069] 2 Drug-treated cells

[0070] Vero cells by 1 x 10 4 Cells / well (volume 100μL) were inoculated in 96-well cell culture plates, and cultured for 24h until the confluence of cell wells reached 80%; the infection group was added with 0.55MOI (multiplicity of infection) AdV3 virus, and at the same time, various gradient concentrations of drugs (at 5μM) were added. As the starting concentration, serially diluted 7 gradients by 3 times, with two duplicate wells for each gradient) to a total volume of 200 μL of culture medium (DMEM medium + 2% seru...

Embodiment 3

[0080] Example 3: Evaluation of anti-AdV5 adenovirus activity of BIIB021 in Vero cell line

[0081] 1 cell culture

[0082] After 2 passages of cryopreserved and resuscitated cells, use DMEM medium containing 10% fetal bovine serum and double antibody (penicillin 100U / ml, streptomycin 100μg / ml) for expansion and culture, and the seeding density is not less than 1 ×10 4 cell / ml, passage density not higher than 5×10 4 cells / ml.

[0083] 2 Drug-treated cells

[0084] Vero cells were inoculated into 96-well cell culture plates at 1×104 cells / well (volume 100 μL), and cultured for 24 hours until the confluence of cell wells reached 80%; the infection group was added with 1.1 MOI (multiplicity of infection) of AdV5 virus, and each gradient was added at the same time. The concentration of the drug (starting at 5 μM, serially diluted 3-fold in 7 gradients, each gradient is two replicates) to a total volume of 200 μL of culture medium (DMEM medium + 2% serum + double antibody), in ce...

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Abstract

The invention belongs to the technical field of medicines, and particularly relates to application of BIIB021 in preparation of a medicine for preventing and / or treating adenovirus infection. The invention discloses an application of BIIB021 in preparation of a medicine for inhibiting adenovirus and / or a medicine for preventing and / or treating adenovirus infection, thereby providing a safe and effective small molecule compound for clinically treating adenovirus. The BIIB021 can effectively inhibit the replication of the adenovirus in a non-toxic range, can be further developed into a medicine for treating or preventing diseases caused by adenovirus infection, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more particularly relates to the application of BIIB021 in the preparation of medicines for preventing and / or treating adenovirus infection. Background technique [0002] Human adenovirus (HAdV) belongs to the mammalian genus Adenovirus of the family Adenoviridae. Adenovirus is a non-enveloped DNA virus with an icosahedral nucleocapsid. The core of the viral genome is a linear double-stranded DNA molecule, about 36kb, and its genome is highly concentrated and packaged, and is packaged by hundreds of similar The histone protein VII and the protamine-like protein Mu (also known as protein X) organize into chromatin. The capsid of adenovirus has 240 hexons, and the 12 tips of the icosahedral capsid are complexes composed of penton and fiber. Twelve fiber proteins protrude from the capsid surface with penton protein as the base, and the top of the fiber forms the knob. Knob regions of penton ...

Claims

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Application Information

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IPC IPC(8): A61K31/52A61P31/20A61P11/00A61P1/00A61P27/02
CPCA61K31/52A61P31/20A61P11/00A61P1/00A61P27/02
Inventor 陈绪林吴建国刘敏丽
Owner JINAN UNIVERSITY
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