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Non-targeted analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology

An analysis method and technology of tobacco products, applied in the direction of analyzing materials, measuring devices, material separation, etc., can solve the problems of limited number of chemical components, insufficient detector sensitivity, unfavorable chemical components, etc.

Pending Publication Date: 2021-04-20
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of tobacco and tobacco products, restricted by sample matrix interference and insufficient detector sensitivity, the number of chemical components covered by conventional targeting methods is relatively limited, and generally requires specific or even cumbersome pretreatment techniques, which is not Conducive to a comprehensive and systematic understanding of the chemical composition of tobacco and tobacco products

Method used

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  • Non-targeted analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology
  • Non-targeted analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology
  • Non-targeted analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A non-targeted analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology. The method is to extract the sample with a solvent, centrifuge it, filter it through an organic phase filter membrane, take the filtrate for analysis, and pass the sample through a liquid chromatography column. After separation, enter the quadrupole time-of-flight mass spectrometry to realize the full component analysis of primary and secondary metabolites in tobacco and tobacco products. The specific steps are as follows:

[0023] (1) Sample pretreatment:

[0024] Accurately weigh 20mg of tobacco leaves into a 50mL centrifuge tube with a cover, then accurately add 20mL of 50% methanol + aqueous solution for extraction (that is, methanol and water account for 50% each), vortex for 10 minutes, centrifuge, filter through an organic phase membrane, and take the filtrate for analysis.

[0025] (2) Sample analysis:

[0026] Chromatographic conditions: chromatographic...

Embodiment 2

[0029] A tobacco leaf sample was selected, and the primary and secondary metabolites in the sample were subjected to non-targeted analysis according to the analysis method described in Example 1. The results are shown in Table 1.

[0030] Non-target analysis results of tobacco leaves in the embodiment of table 1

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Abstract

The invention relates to a non-targeted analysis method for detecting tobacco and tobacco products based on a high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) technology, and the method comprises the following steps of: extracting a sample with a solvent, performing centrifuging, conducting filtering with an organic phase filter membrane, taking filtrate for analysis, separating the sample with a liquid chromatographic column, and conducting quadrupole time-of-flight mass spectrometry determination, thus realizing full-component analysis of primary and secondary metabolites in tobacco and tobacco products. According to the method, the HPLC-QTOF MS technology is applied to non-targeted analysis of tobacco and tobacco products for the first time, the method is particularly suitable for analysis of macromolecules and substances difficult to volatilize in the tobacco and tobacco products, and the problem of chromatographic peak analysis is solved by utilizing the characteristic of high flight time mass spectrum resolution. Compared with the prior art, the method has the advantages that the chemical components of the tobacco and tobacco product complex samples can be conveniently, quickly, accurately and comprehensively analyzed, and a reliable, stable and brand-new method is provided for style characteristic analysis and quality safety monitoring of the tobacco and tobacco products.

Description

technical field [0001] The invention belongs to the technical field of tobacco analysis and detection, and in particular relates to a non-target analysis method for detecting tobacco and tobacco products based on HPLC-QTOF MS technology. Background technique [0002] Tobacco is the most widely planted commercial non-food leaf economic crop in the world. It is a hobby consumer product, and its quality determines the economic value of tobacco and tobacco products. Appraisal of the appearance quality and inner quality of tobacco and tobacco products by means of grading factors and smoking evaluation has certain subjectivity and randomness. Considering that the appearance quality and intrinsic quality are the manifestations of tobacco chemical components in appearance characteristics and smoke characteristics, and this performance has certain regularity and universality, tobacco chemical components are internal factors that determine tobacco quality. There are significant diffe...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/60G01N30/72G01N30/89
Inventor 陈满堂王昇陈黎潘立宁刘瑞红余晶晶王冰刘克建谢复炜刘绍锋聂聪刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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