Non-targeting analysis method for detecting tobacco flavors and fragrances based on HPLC-QTOF MS technology

Abstract

A non-targeting analysis method for detecting tobacco flavors and fragrances based on a high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) technology comprises the following steps of: extracting tobacco flavors and fragrances with a solvent, centrifuging the solution, filtering the solutionby using an organic phase filter membrane, taking the filtratefor analysis, separating a sample by using a liquid chromatographic column, and determining the quadrupole time-of-flight mass spectrometry, so that full-component analysis of primary and secondary metabolites in tobacco flavors and fragrances is realized. According to the non-targeting analysis method, the HPLC-QTOF MS technology is applied to non-targeted analysis of the tobacco flavors and fragrances for the first time, the method is particularly suitable for analysis of macromolecules and substances difficult to volatilize in the tobacco flavors and fragrances, and the problem of chromatographic peak analysis is solved by means of the characteristic that the flight time mass spectrum resolution ratio is high. Compared with the prior art, the non-targeting analysis method disclosed bythe invention has the advantages that the chemical components of the complex sample of the tobacco flavors and fragrances can be conveniently, accurately and comprehensively analyzed, and a reliable,stable and brand-new method is provided for analyzing the tobacco flavors and fragrances.

Description

technical field [0001] The invention belongs to the technical field of determination of tobacco flavors and fragrances, and in particular relates to a non-target analysis method for detecting tobacco flavors and fragrances based on HPLC-QTOF MS technology. Background technique [0002] Tobacco flavors and fragrances are of great significance to maintaining the style and characteristics of cigarette products, and are one of the important core technologies of the tobacco industry. Tobacco flavors and fragrances are mostly made from natural flavors, and their quality is affected by factors such as raw materials and production processes. It is difficult to accurately reflect the composition of flavors and fragrances simply by conventional indicators such as acidity, refractive index, miscibility, and total volatile matter. . Since the influence of tobacco flavors and fragrances on cigarette style often comes from the synergistic effect of multiple components, it is very importa...

AI Technical Summary

Problems solved by technology

[0003] At present, gas chromatography-mass spectrometry (GC-MS) or gas chromatography (GC) are mainly used to detect the components of tobacco flavors and fragrances, but these detection methods based on gas chromatographic separation are difficult to effectively detect semi-active substances in tobacco flavors and fragrances. Volatile especially difficult volatile components

Liquid chromatography-mass spectrometry (LC-MS) or liquid chromatography (LC) based on liquid chromatography separation is suitable for the detection of refractory and semi-volatile components in tobacco flavors and fragrances, and mass spectrometry detectors have better However, the components of tobacco flavors and fragrances are very complex, and the incomplete separation of multiple components leads to the problems of large component interference and inaccurate qualitative determination in the determination of primary mass spectrometry

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