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High performance liquid chromatography-quadrupole flight time mass spectrometry detection method for non-targeted analysis of buccal tobacco

A technology of time-of-flight mass spectrometry and high-performance liquid chromatography, which is applied in the direction of analyzing materials, measuring devices, and material separation. It can solve problems such as complexity, limited number of chemical components, and insufficient detector sensitivity. The effect of high sample measurement and analysis efficiency

Pending Publication Date: 2021-04-20
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex composition of snus, restricted by sample matrix interference and insufficient detector sensitivity, the number of chemical components covered by conventional targeting methods is relatively limited, and generally requires specific pre-processing techniques, which is not conducive to a comprehensive and systematic understanding Chemical composition of snus

Method used

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  • High performance liquid chromatography-quadrupole flight time mass spectrometry detection method for non-targeted analysis of buccal tobacco
  • High performance liquid chromatography-quadrupole flight time mass spectrometry detection method for non-targeted analysis of buccal tobacco
  • High performance liquid chromatography-quadrupole flight time mass spectrometry detection method for non-targeted analysis of buccal tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry detection method for non-targeted analysis of snus, characterized in that: the method is to extract the snus with a solvent, centrifuge it, and filter it through an organic phase membrane to obtain The filtrate is analyzed, and the sample is separated by a liquid chromatography column and then enters a quadrupole time-of-flight mass spectrometer for determination to realize non-targeted analysis of chemical components in snus. The specific steps are as follows:

[0024] (1) Sample pretreatment:

[0025] Take the chemical-type snus and grind it into powder, accurately weigh 20mg of the sample into a 50mL centrifuge tube with a cover, then accurately add 20mL of 50% methanol + aqueous solution for extraction (that is, methanol and water each account for 50%), and vortex for 10 minutes After centrifugation, filter through an organic phase filter, and take the filtrate for analysis.

[002...

Embodiment 2

[0030] A chemical-containing snus sample was selected, and the chemical components in the sample were analyzed non-targeted according to the analysis method described in Example 1. The results are shown in Table 1.

[0031] Non-targeted analysis results of snus in the embodiment of table 1

[0032]

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[0047]

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Abstract

The invention relates to a high performance liquid chromatography-quadrupole flight time mass spectrometry detection method for non-targeted analysis of buccal tobacco, and the method comprises the following steps of: extracting the buccal tobacco by using a solvent, performing centrifuging, conducting filtering by using an organic phase filter membrane, analyzing the filtrate, separating the sample by using a liquid chromatographic column, and performing determining by using quadrupole flight time mass spectrometry, thus realizing non-targeted analysis of the chemical components in the buccal tobacco. According to the method, the high performance liquid chromatography-quadrupole flight time mass spectrometry technology is applied to non-targeted analysis of buccal tobacco for the first time, the method is particularly suitable for analysis of macromolecules and substances difficult to volatilize in buccal tobacco, and the problem of chromatographic peak analysis is solved by utilizing the characteristic of high resolution of the flight time mass spectrometry. Compared with the prior art, the method disclosed by the invention has the advantages that the chemical components of a complex sample of the buccal tobacco can be conveniently, quickly, accurately and comprehensively analyzed, and a reliable, stable and brand-new method is provided for style characteristic analysis and quality safety monitoring of the buccal tobacco.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection of novel tobacco products, and in particular relates to a high performance liquid chromatography-quadrupole time-of-flight mass spectrometry detection method for non-targeted analysis of snus. Background technique [0002] Snus is a smokeless tobacco product consumed by mouth, and it is one of the main forms of new tobacco products. Affected by the current situation of severe tobacco control in the world, snus is consumed through the oral cavity and digestive system without burning, so that consumers can effectively avoid exposure to traditional tobacco combustion products and will not affect the public and the environment. Important development trends in the international tobacco field. The chemical composition of snus is more complex than that of traditional tobacco products, mainly including the inherent chemical composition of tobacco and additive ingredients, among which the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/60G01N30/72G01N30/89
Inventor 王昇陈满堂陈黎王晓瑜郭军伟崔华鹏樊美娟秦亚琼谢复炜刘绍锋聂聪刘惠民
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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