Fluorescence immunoassay quantitative POCT detection method for mycotoxin in agricultural products
A fluorescent immunity and mycotoxin technology, which is applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of high detection cost, complex detection process, and difficult to popularize, and achieve accurate detection results, improve detection efficiency, and improve linearity. range effect
Pending Publication Date: 2021-04-20
无锡精检生物技术有限公司
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Problems solved by technology
[0005] The technical problem to be solved by the present invention is: the existing method for detecting mycotoxins in agricultural products is either complicated in the detection process, or high in detection cost, and difficult to popularize
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[0021] The invention provides a method for detecting mycotoxin fluorescence immunoquantitative POCT in agricultural products, comprising the following steps:
[0022] a. Sample pretreatment
[0023] Take an agricultural product sample, crush it and pass it through a 100-mesh sieve, take a certain amount of sample, add 10-20 times the amount of extraction solution, and extract by vortexing or shaking for 10 minutes; Add to 500μl sample diluent, mix well, and prepare the solution to be tested;
[0024] b. Sample testing on machine
[0025] Take out the fluorescent immunoquantitative POCT test strip, and use the fluorescent immunoquantitative POCT analyzer for detection. After the temperature of the constant temperature incubator rises to 37°C and stabilize, the test starts. Take out the fluorescent immunoquantitative POCT test strip, and place it horizontally in the constant temperature incubator for heating On the board, absorb 100 μl of the solution to be tested obtained in ...
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The invention belongs to the technical field of agricultural product detection, and particularly relates to a fluorescence immunoassay quantitative POCT (point-of-care testing) detection method for fungaltoxin in agricultural products. In order to solve the problems of complex detection process, high detection cost and difficulty in popularization of the existing method for detecting mycotoxin in agricultural products, the invention provides the fluorescence immunoassay quantitative POCT detection method for mycotoxin in the agricultural products, which comprises the following steps: a, taking a certain amount of sample, adding 10 times of extracting solution, and extracting for 5-10 minutes to prepare a solution to be detected; b, detecting by adopting a fluorescence immunoassay quantitative POCT analyzer, sucking 100 microliters of the solution to be detected obtained in the step a, adding the solution to be detected into a sample adding hole of a test strip, covering a cover, and incubating for 8 minutes; after 8 minutes, inserting the test strip into a test strip slot of the fluorescence immunoassay quantitative POCT analyzer, and reading; and c, judging a detection result. The detection method disclosed by the invention is wide in application range, high in detection sensitivity and suitable for popularization and application.
Description
technical field [0001] The invention belongs to the technical field of agricultural product detection, and in particular relates to a method for detecting mycotoxin fluorescence immunoquantitative POCT in agricultural products. Background technique [0002] During the storage of agricultural products, they are easily infected by various molds, and some mycotoxins may remain in the agricultural products. Such agricultural products will seriously endanger the health of consumers when they are put on the market. [0003] Mycotoxins are small molecular secondary metabolites produced by fungi. Crops are susceptible to mycotoxin contamination during growth, harvesting, storage and transportation. More than 400 types of mycotoxins have been found, most of which are carcinogenic and teratogenic. and mutagenic effects, and the toxicity will be significantly enhanced when mixed pollution. Mycotoxins can cause serious harm to human and animal health by contaminating grain and feed or ...
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IPC IPC(8): G01N33/58G01N33/558G01N33/53
Inventor 汪劲能
Owner 无锡精检生物技术有限公司