Novel application of neferine to inhibition of SARS-CoV and SARS-CoV-2 infection

A technology of lysinine and sars-cov-2, applied in the field of natural medicine, can solve the problems of many potential hosts and long incubation period, etc.

Active Publication Date: 2021-04-23
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a highly infectious virus, the new coronavirus SARS-CoV-2 has the characteristics of certain mutation, multiple potential hosts, and long incubation period, and many other characteristics have yet to be elucidated

Method used

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  • Novel application of neferine to inhibition of SARS-CoV and SARS-CoV-2 infection
  • Novel application of neferine to inhibition of SARS-CoV and SARS-CoV-2 infection
  • Novel application of neferine to inhibition of SARS-CoV and SARS-CoV-2 infection

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Experimental program
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Embodiment Construction

[0024] 1) Construct SARS-CoV, SARS-CoV-2, VSV-G pseudovirus system

[0025] The SARS-CoV, SARS-CoV-2 and VSV-G pseudovirus systems were constructed using the three-plasmid packaging system of lentivirus. The pseudovirus system uses SARS-CoV or SARS-CoV-2 spike protein or VSV G protein as surface membrane protein, and firefly luciferase as reporter gene. Using GenJet Ver.II transfection reagent, spike envelope plasmid or G protein envelope plasmid, packaging plasmid psPAX2, and HIV plasmid containing luciferase reporter gene were co-transfected into HEK293T cells, and fresh culture medium was replaced after 12 hours. After 24, 36, 48, and 60 hours of transfection, collect the supernatant culture solution, centrifuge it at 800×g for 5 minutes, and then filter it through a 0.45 μm filter membrane to remove cell debris. The obtained pseudovirus is subpackaged and stored in - 80°C.

[0026] 2) The whole process of administration of nexinine to study its inhibitory effect on HEK29...

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Abstract

The invention discloses an application of neferine to inhibition of SARS-CoV and SARS-CoV-2 infection, and belongs to the technical field of natural medicines. Experimental results show that the neferine (Nef) can be used for safely and effectively inhibiting infection of SARS-CoV and SARS-CoV-2 pseudovirus particles on host cells. Along with the increase of the concentration of the neferine, the infection rate of pseudovirus particles to cells is gradually reduced. The median inhibitory concentration (IC50) of the neferine acting on the SARS-CoV pseudovirus is 0.43[mu]M, and the IC50 of the neferine acting on the SARS-CoV-2 pseudovirus is 0.36 [mu]M. In addition, the CCK8 result shows that the median toxicity concentration (CC50) of the neferine is 29.17 [mu]M, and the calculated selectivity indexes (SI=CC50 / IC50) of the neferine are 67.84 and 81.03, respectively. Through administration at different time points and a VSV-G pseudovirus elimination experiment, the infection stage of the neferine acting on the SARS-CoV and the SARS-CoV-2 is confirmed. The research proves that the neferine can be used as a candidate inhibitor for SARS-CoV and SARS-CoV-2 infection, and the new application of the neferine as a clinical treatment medicine and preparation is shown.

Description

technical field [0001] The invention relates to the technical field of natural medicines, and specifically provides the application of liensinine as an inhibitor in anti-SARS-CoV and SARS-CoV-2 infection. Background technique [0002] Lien Tze Hsin (Lien Tze Hsin) is one of the commonly used Chinese medicinal materials in my country. It is the germ of the mature seeds of Nelumbo nucifera Gaertn., which was first seen in "Food Habits Materia Medica". The lotus seeds are cold in nature and bitter in taste. They have the functions of clearing away heat, consolidating essence, calming the nerves, and strengthening the heart. Pharmacological studies have shown that the main components of lotus seed heart are alkaloids, such as liensinine, isoliensinine, and methyl-liensinine. [0003] Neferine is a bisbenzylisoquinoline alkaloid with the molecular formula C 38 h 44 N 2 o 6 , the molecular weight is 624.778g / mol, and its chemical structure is as follows. [0004] [0005]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4725A61K36/62A61P31/14A61P11/00
CPCA61K31/4725A61K36/62A61P31/14A61P11/00Y02A50/30
Inventor 王升启王学军杨扬杨鹏吴玉明
Owner ACADEMY OF MILITARY MEDICAL SCI
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