Commodity matching recommendation method based on carrier construction, storage medium and electronic equipment
A recommendation method and carrier technology, applied in the analysis of two-dimensional or three-dimensional molecular structure, genomics, proteomics, etc., can solve problems such as reducing the purchase rate and affecting the user's product purchase experience
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example 1
[0064] Example 1: When the vector is: mammalian double gRNA expression vector (pRP_dual_gRNA)
[0065] The two specific RNAs encoded by the vector can guide Cas9 nuclease or Cas9_D10A nickase to edit the DNA sequence of two specific sites in the genome.
[0066] In the cell, the Cas9 nuclease forms a complex with the guide RNA (gRNA), which directly interacts with the 18-22nt homologous target sequence in the genome, and the gRNA and the target site make Cas9 localize to the target through complementary pairing sequence, and then cleave the target site in the genome. Because the specificity of two gRNAs is required, the gRNA in this vector has less off-target effects when targeting the same gene. At the same time, two gRNA targeting DNA sequences can know that Cas9 knocks out a large sequence on the gene to inactivate the gene, so as to achieve a more efficient gene knockout effect.
[0067] The vector itself does not express the Cas9 protein, and needs to be used with an ex...
example 2
[0071] Example 2: When the vector is: mammalian gene FLEX conditional expression AAV vector (Cre-On): pAAV_FLEX_R
[0072] The vector uses the FLEX Cre-on conditional gene expression system to conditionally activate the expression of the target gene through Cre recombinase. The FLEX Cre-on system uses the Lox-variant recombination sites located on both sides of the target gene. Under the regulation of Cre recombinase, the target gene is reversed, and the target gene is normally expressed; in the absence of Cre, the target gene is not expressed. .
[0073] The FLEX Cre-on system consists of two pairs of heterotypic Lox-variant recombination sites, where the wild-type sequence is called LoxP, and the mutant is called Lox2272, which are located on both sides of the reverse gene ORF (relative to the direction of the promoter). Both Lox-variants are recognized by Cre, but only the same pair of Lox sites can recombine with each other, not with other Lox-variants. LoxP and Lox2272 ...
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