A kind of breeding method and application of Culicoides
A breeding method and technology of breeding cages, which are applied to the breeding method of Culicoides and its application fields, can solve the problems that it is difficult to ensure a sufficient number of Culicoides with strong vitality at any time, it is not suitable for large-scale breeding, and the number of operations is too many, etc., to achieve Shorten the research and development cycle, save manpower and time, and increase the effect of egg production
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Embodiment 1
[0062] The cultivation method of the Culicoides of the present embodiment may further comprise the steps:
[0063] Hatching: place a layer of 80-mesh nylon cloth 310 on the stainless steel shelf 211 of the hatching basin 210 in the culture device, add water (the water added in the hatching basin is tap water that has been standing for 2 days) until the water surface is flush with the stainless steel shelf 211, but not completely Pass through nylon cloth 310, water depth 7 cm, get 5 g of larval food material A and put it on nylon cloth 310, turn on the air pump, inject air into the water through air stone 212, and form a microenvironment suitable for the life of Culicoides larvae in the hatching basin 210 after 4 days, Put the filter paper 1200 containing Culicoid eggs on the nylon cloth 310 in the hatching basin 210, control the temperature at 28°C and the humidity at 80%, and hatch and raise them to obtain larvae;
[0064] Larva rearing: After the larvae are observed, add lar...
Embodiment 2
[0068] The cultivation method of the Culicoides of the present embodiment may further comprise the steps:
[0069] Hatching: place a layer of 80-mesh nylon cloth 310 on the stainless steel shelf 211 of the hatching basin 210 in the culture device, add water (the water added in the hatching basin is tap water that has been standing for 2 days) until the water surface is flush with the stainless steel shelf 211, but not completely Pass through nylon cloth 310, water depth 7 cm, get 5 g of larval food material A and put it on nylon cloth 310, turn on the air pump, inject air into the water through air stone 212, and form a microenvironment suitable for the life of Culicoides larvae in the hatching basin 210 after 4 days, Put the filter paper 1200 containing Culicoid eggs on the nylon cloth 310 in the hatching basin 210, control the temperature at 28°C and the humidity at 80%, and hatch and raise them to obtain larvae;
[0070] Larva rearing: After the larvae are observed, add lar...
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