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Bactericide and application thereof

A technology of bactericide and chelating agent, which is applied in the field of bactericide and its application, can solve the problem of inability to kill Gram-negative bacteria, etc., and achieve the effect of broadening the spectrum of antibacterial activity, significant antibacterial effect, and extensive antibacterial activity

Inactive Publication Date: 2021-04-30
山东元泰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nisin, however, cannot kill Gram-negative bacteria because the plasma membrane of Gram-negative bacteria is protected by an outer covering of lipopolysaccharide (Asaduzzaman and Sonomoto (2009) J.Biosci.Bioeng.107:475-87)

Method used

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  • Bactericide and application thereof
  • Bactericide and application thereof
  • Bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The bactericidal effect of nisin and chelating agent sodium gluconate on Gram-negative bacteria Escherichia coli as follows figure 1 shown.

[0028] Under the conditions of 37°C and pH 6.0, a control was set up for the test, and the initial viable count of Escherichia coli was 10 5 CFU / ml. After 24 hours, the bacterial survival rate was used as an index to determine the effect of the fungicide. In the control group without adding sodium gluconate, the killing effect of nisin on Escherichia coli was not obvious. However, the treatment group added sodium gluconate showed strong activity against E. coli. The activity increased with increasing nisin concentration. The combination of nisin and sodium gluconate as a bactericide was shown to increase effectiveness against Escherichia coli. Therefore, it can be considered that the fungicide containing Nisin and the chelating agent sodium gluconate is an effective food preservative against various Gram-negative bacteria.

Embodiment 2

[0030] Bactericidal effect of nisin and chelating agent sodium gluconate on Gram-negative bacteria Klebsiella pneumoniae (Klebsiellapneumoniae)

[0031] Under the conditions of 37°C and pH 6.0, the initial bacterial concentration was 10 5CFU / ml, the composition containing Nisin and sodium gluconate and Nisin alone are used to evaluate the killing effect of Gram-negative bacteria Klebsiella pneumoniae ( figure 2 ). In the absence of sodium gluconate, Nisin has no obvious bactericidal effect on Klebsiella pneumoniae. However, the fungicide showed significant activity against Klebsiella pneumoniae with the addition of sodium gluconate. The bactericidal activity increased with the increase of nisin concentration.

Embodiment 3

[0033] Bactericidal effect of nisin and chelating agent sodium gluconate on gram-negative bacteria Salmonella typhimurium (S.typhimurium)

[0034] The killing activity of Nisin and sodium gluconate against Gram-negative bacteria Salmonella typhimurium image 3 shown. The Nisin control group without sodium gluconate had no obvious activity against typhoid fever. However, when Nisin was used in combination with sodium gluconate, the fungicide showed significant activity against Salmonella typhimurium. The enhancement effect of sodium gluconate on nisin was concentration-dependent.

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Abstract

The invention discloses a bactericide and an application thereof, and relates to the field of industry, and the bactericide is formed by mixing lanothionein and a chelating agent. According to the embodiment of the invention, the antibacterial peptide composition is innovatively designed, so that the antibacterial peptide composition has a remarkable antibacterial effect and a unique antibacterial mechanism, the antibacterial activity spectrum of the peptides is widened, and new prospects of the peptides in the aspect of additive foods are developed. A chelating agent sodium gluconate can destabilize the outer membrane of the gram-negative bacteria and form holes in the mycoderm, so that the composition has the activity of resisting the gram-negative bacteria. The composition is capable of inducing instability of bacterial walls and providing a broad range of antibacterial activity without requiring pretreatment or purification prior to use.

Description

technical field [0001] The invention relates to the industrial field, in particular to a bactericide and its application. Background technique [0002] Lantibiotics are protein toxoids synthesized by certain bacterial ribosomes and have antibacterial activity against other types of bacteria. As an antibacterial polypeptide, nisin is a widely used, effective and safe food additive. It is a food preservative approved by the European Union (E234 food additive) and FDA. In addition, more than 50 countries have applied Nisin to food preservation and food preservation, with a history of nearly 70 years. Due to the superior stability and effective mechanism of bacterial antimicrobial peptides, it is considered that they can be used to design a new generation of natural source antibiotics (Clardy et al. (2006) Nat. Biotechnol. 24:1541-1550). [0003] The bacteriostatic mechanism of antimicrobial peptides strongly depends on the interaction with bacterial membranes (Breukink et al....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3535A23L3/3499
CPCA23L3/3535A23L3/3499A23V2002/00
Inventor 张德宁奥兰多徐洪利高祥友
Owner 山东元泰生物工程有限公司