Application of tauroursodeoxycholic acid in treatment of neonatal necrotic enterocolitis

A technology for tauroursodeoxycholic acid and enterocolitis, which is applied in the field of medicine and can solve the problems of unclear preventive and therapeutic effects of tauroursodeoxycholic acid, achieve good protection, improve survival rate, and reduce apoptosis. Effect

Pending Publication Date: 2021-05-07
付东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If traditional drugs are used for clinical NEC intervention, it may cause certain side effects. Therefore, it is urgent to find a safe and effective drug or method for the prevention and treatment of necrotizing enterocolitis
[0007] However, the preventive and therapeutic effects of the above-mentioned tauroursodeoxycholic acid (TUDCA) in neonatal diseases are still unclear.

Method used

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  • Application of tauroursodeoxycholic acid in treatment of neonatal necrotic enterocolitis
  • Application of tauroursodeoxycholic acid in treatment of neonatal necrotic enterocolitis
  • Application of tauroursodeoxycholic acid in treatment of neonatal necrotic enterocolitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] In this embodiment, as figure 1 In the modeling process shown, a 5-day-old C57BL / 6 neonatal mouse model of necrotizing enterocolitis was prepared by artificial feeding combined with hypoxia and cold stimulation. The experiment was divided into three groups: normal control group (10 rats), neonatal necrotizing enterocolitis model group (15 rats), and neonatal necrotizing enterocolitis model TUDCA intervention group (20 rats).

[0036] The specific experimental process is as follows:

[0037] Mice were artificially fed: Rat milk substitutes such as petag brand milk powder were used, the total energy was 840 kJ / kg / d, the feeding amount was 50 μl / g, and feeding was given at regular intervals of 4 hours.

[0038] Hypoxia and cold stimulation: hypoxia and cold stimulation were controlled within 1 hour after the meal. First place them in a hypoxic bottle containing 100% nitrogen for 60 seconds, then place them in a refrigerator at 4°C for 10 minutes, and cold-stimulate them ...

Embodiment 2

[0053] In this example, an in vitro experiment on the IEC-6 cell line was carried out to further verify the effect of TUDCA.

[0054] IEC-6 was grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, according to 1×10 6 Cells / mL concentration was inoculated into 6-well cell culture plate, 37°C, 5% CO 2 Cultured for 12 hours to allow the cells to fully adhere to the wall. LPS and TUDCA were prepared using normal saline. IEC-6 cells were treated with lps (50 μg / ml) for 6 hours, and TUDCA was added to the final concentration of 50 μM, 100 μM, and 200 μM, and then the cells were collected for subsequent research. Figure 8 Photographs for IEC-6 cell cultures, such as Figure 8 As shown in part B of , lps induces increased IEC-6 cell death, as Figure 8 As shown in part C, the intervention of TUDCA can improve the death of IEC-6 and promote the survival of cells. The results of the above in vitro experiments are as follows: Figures 9 to 11 shown...

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Abstract

The invention provides a new application of tauroursodeoxycholic acid (TUDCA) or pharmaceutically acceptable salts thereof in preparation of drugs for prevention or treatment of neonatal necrotic enterocolitis. TUDCA plays a good protective role on endoplasmic reticulum stress caused by neonatal Necrotic Enterocolitis (NEC), CHOP and BiP are significantly reduced under the action of TUDCA, TUDCA intervention can maintain the weight of NEC model newborn mice, reduce intestinal epithelial cell apoptosis, and improve the survival rate of NEC model newborn mice. Through cell and apoptosis level experiments, TUDCA is confirmed to play a protective role on NEC by inhibiting endoplasmic reticulum stress and reducing the cell apoptosis proportion in NEC, and lays a theoretical foundation for prevention and treatment of neonatal necrotic enterocolitis by TUDCA.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of taurursodeoxycholic acid in the treatment of neonatal necrotizing enterocolitis. Background technique [0002] Necrotizing enterocolitis (NEC) of the newborn is one of the most serious and common neonatal diseases and the leading cause of death from gastrointestinal diseases in premature infants. Currently, among very low birth weight infants in China, the incidence of neonatal necrotizing enterocolitis is 4.53%, and the mortality rate related to neonatal necrotizing enterocolitis is as high as 41.7%. The occurrence and development of neonatal necrotizing enterocolitis involves a complex pathophysiological process of intestinal immaturity, abnormal bacterial colonization and excessive immune response in preterm infants. Since the exact etiology and pathogenesis are unclear, there are still no effective preventive and therapeutic measures in clinical practice,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575A61P1/00A61P29/00
CPCA61K31/575A61P1/00A61P29/00
Inventor 付东李朋
Owner 付东
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