A preparation method of macroporous-large mesoporous-small mesoporous tertiary porous silica with high enzyme immobilization capacity
A porous silica, immobilization technology, applied in the field of enzyme immobilization, can solve the problems of low immobilization efficiency, enzyme leaching, etc.
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example 1
[0019] Dissolve a certain amount of P123 (triblock copolymer template agent) in deionized water, and configure it into a 10% mass fraction of P123 aqueous solution for later use; weigh 20g of the resulting solution, add 60g deionized water and 11.9g mass fraction to make 37% of concentrated hydrochloric acid, 0.1g of polystyrene microspheres (PS), and then 2.55g of ethyl orthosilicate (TEOS) was added and placed in a water bath, magnetically stirred, and the temperature was maintained at 43 °C; After TEOS, the same amount of TEOS was added again at an interval of 3.5 hours, and the water bath was continued for 24 hours; after the water bath was over, the obtained solution was transferred to the reaction kettle, hydrothermally aged for 24 hours, and the hydrothermal temperature was kept at 100 °C; after the reaction, the obtained product was centrifuged, The supernatant was removed, and the precipitate was repeatedly suction filtered, washed, and centrifuged until the pH of the ...
example 2
[0021] Dissolve a certain amount of P123 (triblock copolymer template agent) in deionized water, and configure it into a 10% mass fraction of P123 aqueous solution for later use; weigh 20g of the resulting solution, add 60g deionized water and 11.9g mass fraction to make 37% of concentrated hydrochloric acid, 0.1g of polystyrene microspheres (PS), and then 2.55g of ethyl orthosilicate (TEOS) was added and placed in a water bath, magnetically stirred, and the temperature was maintained at 43 °C; After TEOS, the same amount of TEOS was added again at an interval of 3.5 hours, and the water bath was continued for 24 hours; after the water bath, the obtained solution was transferred to the reaction kettle, and hydrothermally aged for 24 hours, keeping the hydrothermal temperature at 110 °C; after the reaction, the obtained product was centrifuged, The supernatant was removed, and the precipitate was repeatedly suction filtered, washed, and centrifuged until the pH of the supernatan...
example 3
[0023] Dissolve a certain amount of P123 (triblock copolymer template agent) in deionized water, and configure it into a 10% mass fraction of P123 aqueous solution for later use; weigh 20g of the resulting solution, add 60g deionized water and 11.9g mass fraction to make 37% of concentrated hydrochloric acid, 0.1g of polystyrene microspheres (PS), and then 2.55g of ethyl orthosilicate (TEOS) was added and placed in a water bath, magnetically stirred, and the temperature was maintained at 43 °C; After TEOS, the same amount of TEOS was added again at an interval of 3.5 hours, and the water bath was continued for 24 hours; after the water bath, the obtained solution was transferred to the reaction kettle, and hydrothermally aged for 24 hours, keeping the hydrothermal temperature at 120 °C; after the reaction, the obtained product was centrifuged, The supernatant was removed, and the precipitate was repeatedly suction filtered, washed, and centrifuged until the pH of the supernatan...
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