Cultivation method and application of tomato grafted seedlings

A cultivation method and technology for grafting seedlings, which are applied in the field of cultivation of tomato grafted seedlings, can solve the problems of loss of Mi resistance, threatening the persistent utilization of resistance genes, etc., and achieve the effect of preventing and controlling harm

Active Publication Date: 2021-05-25
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term use of a single Mi resistance gene for nematode resistance breeding in tomato breeding for many years, the root-knot nematode virulence population has been differentiated, and the root-knot nematode has overcome the resistance, resulting in the loss of Mi resistance, which seriously threatens the resistance. persistent use of genes

Method used

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  • Cultivation method and application of tomato grafted seedlings
  • Cultivation method and application of tomato grafted seedlings
  • Cultivation method and application of tomato grafted seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the grafting technique of anti-root-knot nematode capsicum and tomato

[0025] 1. A grafted tomato seedling cultivated by the grafting cultivation method provided by the present disclosure:

[0026] Grafting preparation: The root-knot nematode-resistant “HDA149” variety pepper and the “Lichun” variety tomato seeds were disinfected with 10% sodium hypochlorite, rinsed with sterile water and accelerated germination. Capsicum seeds and tomato seeds are sown in stages, wherein the sowing period of tomato seeds is 15 days later than that of capsicum seeds. Use sterilized peat: vermiculite (2:1) as the culture substrate, and manage the seedlings at a relative humidity of 70-80% and a temperature of 25-28°C. About 45 days after the emergence of pepper seedlings, the pepper seedlings reached the stage of 4-5 true leaves, and the thickness of the stems of the pepper seedlings was about 2.5-3.0mm at this time; while the tomato seedlings reached the stage of 3 leave...

Embodiment 2

[0043] Embodiment 2: the pot experiment of tomato grafted seedling

[0044] 1. Experimental materials:

[0045] In the pot experiment of tomato grafted seedlings, a total of 6 groups were set up, with 20 tomato seedlings in each group. Among them, there are 5 experimental groups and 1 control group. In the experimental group, tomato seedlings of the "Lichun" variety grafted with "HDA149" pepper as rootstock were cultivated at a temperature of 25°C; tomato seedlings of "Lichun" variety were grafted with "HDA149" pepper as rootstock , and cultivated at a temperature of 32°C; tomato seedlings of the "Lichun" variety grafted with the "rootstock Guozhen No. 1" tomato as a rootstock, and cultivated at a temperature of 25°C; The variety tomato is the "Lichun" variety tomato seedling grafted on the rootstock, and cultivated at a temperature of 32°C; the ungrafted "Lichun" variety tomato seedling is treated with 10% thiazophosphine (Fufuduo) for chemical control. And the ungrafted t...

Embodiment 3

[0064] Embodiment 3: the field experiment of tomato grafted seedling

[0065] 1. Experimental materials:

[0066] The field experiment was carried out in the greenhouse of the experimental base in Langfang City, Hebei Province. The occurrence of root-knot nematodes in this field is serious, and the distribution of root-knot nematodes in the field is even. In 100g of soil, the average density is 22-30.

[0067] In the field experiment of tomato grafted seedlings, 4 groups were set up, with 30 tomato seedlings in each group. Among them, there are 3 experimental groups and 1 control group. In the experimental group, the tomato seedlings of the "Lichun" variety were grafted with the "HDA149" variety pepper as the rootstock; the tomato seedlings of the "Lichun" variety were grafted with the "rootstock Guozhen No. 1" tomato as the rootstock; There are many blessings) ungrafted tomato seedlings of the "Lichun" variety that were treated with chemical control. And the ungrafted toma...

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Abstract

The invention relates to a cultivation method and application of tomato grafted seedlings. The cultivation method comprises the following steps of (1) respectively disinfecting and accelerating germination of root-knot nematode-resistant pepper seeds and tomato seeds, sowing by stages, and cultivating root-knot nematode-resistant pepper seedlings and tomato seedlings; (2) selecting the root-knot nematode-resistant pepper seedlings as stocks and the tomato seedlings as scions when the root-knot nematode-resistant pepper seedlings reach a period of 4-5 true leaves and the tomato seedlings reach a period of 3 leaves and 1 core, processing the middle of the section of a tender stem of each root-knot nematode-resistant pepper seedling to form a cleaved notch, processing a cotyledonary node of each tomato seedling to form a tip, and performing grafting, specifically, inserting the tips of the tomato seedlings into the cleaved notches of the root-knot nematode-resistant pepper seedlings to be aligned and tightly attached, using grafting clamps for clamping the joints, and completing grafting; and (3) after completing grafting, spraying tomato grafted seedlings, putting the tomato grafted seedlings into a plastic arched shed for moisturizing, culturing for 2-3 weeks after grafting, transplanting and planting, and carrying out production management. The tomato grafted seedlings cultivated by the cultivation method disclosed by the invention has a root-knot nematode prevention and control effect of 100%.

Description

technical field [0001] The disclosure relates to the technical field of vegetable seedling cultivation, in particular to a cultivation method and application of grafted tomato seedlings. Background technique [0002] Root-knot nematodes have a wide range of hosts, and their damage is common to horticultural crops, especially solanaceae, cucurbitaceae, and cruciferous plants. The most common ones are M. incognita, M. javanica, M. arenaria and M. hapla, which are extremely difficult to control. soil-borne diseases. [0003] Since root-knot nematode-resistant genes have been found in tomatoes and peppers, for tomato and pepper and other Solanaceous crops, the strategy of nematode-resistant breeding is mainly adopted for the control of root-knot nematodes, which is safe and efficient. [0004] Mi resistance gene is an important tomato disease and pest resistance gene. However, due to the long-term use of a single Mi resistance gene for nematode resistance breeding in tomato b...

Claims

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Application Information

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IPC IPC(8): A01G2/30A01G2/35A01G22/05A01G13/00A01G24/15A01G24/28A01C1/02A01C1/08
CPCA01G2/30A01G2/35A01G22/05A01G13/00A01G24/15A01G24/28A01C1/02A01C1/08
Inventor 茆振川谢丙炎赵建龙杨宇红凌键李彦
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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