New method for monitoring grain mildew by detecting mildew in aerosol in grain storage environment and application

An aerosol and new method technology, applied in the direction of microbial-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of polluted grain, grain mildew, and failure to meet early warning, etc., to achieve prevention and control Effects of mold hazards

Pending Publication Date: 2022-04-22
DALIAN NATIONALITIES UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the study, it was found that the existing monitoring methods for grain mildew cannot meet the needs of early warning. 2 or mycotoxins, the food has already undergone severe mildew, and molds and their toxins have polluted most of the food; a new technology is urgently needed to solve the above problems

Method used

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  • New method for monitoring grain mildew by detecting mildew in aerosol in grain storage environment and application
  • New method for monitoring grain mildew by detecting mildew in aerosol in grain storage environment and application
  • New method for monitoring grain mildew by detecting mildew in aerosol in grain storage environment and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Establishment of Corn Mildew Model and Determination of Mold Quantity

[0043] 1.1 Establish a model of mold-contaminated corn, build a simulation test chamber for 500g of grain, and a corn mildew simulation chamber, spray sterile water on the surface of the grain, seal it with a plastic film after fully wetting, and test it after 5 days.

[0044] 1.2 According to the GB / T4789.15-2016 method, weigh 500g of corn samples every day for mold counting, and refer to the GB / T4789.16-2016 method for mold identification.

[0045] 1.3 Use an automatic aerosol monitor to collect moldy sample aerosol in a confined space. The aerosol sample is incubated at 30°C, and the mold count and type identification are also carried out according to the methods of GB / T4789.15-2016 and GB / T4789.16-2016 .

[0046] The study found that in the early stage of mildew, the physiological effect of the mold is very weak, and the detectable aerosol gas concentration is relatively low; as time ...

Embodiment 2

[0047] Example 2 Correlation Test of Grain Mildew Degree and Mold Concentration in Aerosol

[0048] Take 25 grams of moldy samples for mold counting, and at the same time collect and culture the molds in the moldy grain aerosol. Use 10mL Sabouraud liquid medium to collect the mold in the aerosol of grain mildew environment, culture it with Bengal red agar medium, cultivate it at 30°C for 5 days and count it. Record the number of molds in the mildew samples every day, and record the number of molds in the mold aerosol samples at the same time.

[0049] Table 1 Changes in the number of molds in mildew samples and aerosol samples

[0050]

[0051] The invention provides a mold counting method for grain mildew monitoring, which simultaneously counts the mold collected in the aerosol and the mold in the moldy sample, and judges whether the concentration of the mold in the aerosol is the same as that in the moldy sample, Then judge whether the mold detection in the aerosol can ...

Embodiment 3

[0053] Example 3 Establishment of Real-time Fluorescent PCR Detection Method for Main Molds in Aerosol and Grain Mildew Samples

[0054] 3.1 Primer and probe design

[0055] Primers and probes were designed according to the specific species identification genes of the main molds in grain mildew, Aspergillus flavus, Aspergillus fumigatus, Fusarium graminearum, Fusarium verticillium, and Ochrae sp., and the primers and probes were determined by blast comparison on GenBank The specificity of needles, primers and probes were synthesized by Bao Biological Engineering (Dalian) Co., Ltd. The sequences of primers and probes are listed in Table 2.

[0056] Table 2 Real-time fluorescent PCR primer and probe sequences

[0057]

[0058] 3.2 Extraction of mold genomic DNA

[0059] Take 50 μL of microbial lysis buffer and add it to a sterilized EP tube. Pick a single colony of mold on Bengal red agar, put it into an EP tube and stir it evenly. 80°C, 15min, 2000r / min low-speed centri...

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Abstract

The invention discloses a novel method for monitoring grain mildewing by detecting mould in aerosol in a grain storage environment and application, and particularly relates to a method for collecting mould in the aerosol in the environment in a grain storage and production processing place, researching the relationship between mould activity and grain mildewing and carrying out early warning and monitoring on grain mildewing. The method comprises the following steps: collecting mould in aerosol generated in ambient air due to mildew of stored grains by using a biological aerosol monitor, counting the mould in the aerosol, quickly analyzing the types of the mould in the aerosol generated by the mildew of the grains, and establishing a mould molecular biology quick identification method. The invention provides and establishes a new thought and a new method for early warning and monitoring grain mildewing by collecting and monitoring mould in environmental aerosol of a grain storage place. The method provided by the invention has the characteristics of accuracy, rapidness and sensitivity in early monitoring and early warning of grain mildewing.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for collecting and detecting molds in environmental aerosols of grain storage places, and performing early warning and monitoring of grain mildew. Background technique [0002] my country has a large population, and the problem of food shortage is relatively serious. Solving the problem of safe storage of food is a big issue about the national economy and people's livelihood. Mold pollution is an important cause of hazards in the process of food and grain storage, which will reduce the quality of food and grain, reduce the taste, nutrition and other edible value; and the toxin produced by mold will damage the health of humans and animals, and is carcinogenic. When the storage time is too long, the mold is easy to grow and multiply rapidly. According to relevant survey statistics, due to the harm of the mold and the toxin it produces to the stored grain, the annual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12Q1/686C12Q1/04C12R1/67C12R1/68C12R1/645
CPCC12Q1/6895C12Q1/686C12Q1/045C12Q2561/113C12Q2563/107
Inventor 郑秋月杨爱馥曹际娟黄大亮梁冰
Owner DALIAN NATIONALITIES UNIVERSITY
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