Application of marigold volatile matter beta-cyclohomocitral to prevention and treatment of Chinese wolfberry aphids

A technology of wolfberry aphids and citral, which can be applied in the agricultural field and can solve the problems of pollution, residual poisoning, destruction of ecological balance, etc.

Active Publication Date: 2021-05-04
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The long-term and large-scale use of chemical pesticides has brought various disadvantages, such as the generation of pest resistance, residual poisons and environmental pollution, killing a large number of natural enemies of pests regardless of good and bad, seriously destroying the ecological balance

Method used

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  • Application of marigold volatile matter beta-cyclohomocitral to prevention and treatment of Chinese wolfberry aphids
  • Application of marigold volatile matter beta-cyclohomocitral to prevention and treatment of Chinese wolfberry aphids
  • Application of marigold volatile matter beta-cyclohomocitral to prevention and treatment of Chinese wolfberry aphids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, collection and determination of marigold plant volatiles

[0030] The test insects were the wolfberry aphids (Aphis sp.) raised indoors. The wolfberry aphids collected from the wolfberry field in Zhongning, Ningxia, were inoculated on the wolfberry grown in the greenhouse. After two days, the inoculated aphids were removed, and the individual size and insect state were basically the same. wingless adult aphids as test insects

[0031] Samples of volatile compounds from marigold flowers, stems and leaves, and whole plants were collected by dynamic headspace adsorption. The collection device schematic diagram of described marigold volatile matter is as figure 1 shown. Pour the Parpark adsorbent into the adsorption column, elute with 0.5ml of dichloromethane (chromatographically pure), rinse three times, and then concentrate with high-purity nitrogen, and the collected volatile sample is about 0.2ml. Then the concentrated sample is analyzed for the activit...

Embodiment 2

[0037] Example 2, research on the repellent effect of verbenol on wolfberry aphids

[0038]Based on Example 1, this part focuses on the repellent effect of verbenol on Lycium barbarum aphids. The specific research contents include the effect of different concentrations of verbenol on the escape behavior of Lycium barbarum aphids, the response to the olfactory behavior of Lycium barbarum aphids, and the effect on Lycium barbarum aphids. Formation and population individual size effects.

[0039] The data obtained from the experiment are represented by mean ± standard error. The data of the effects of different concentrations of verbenol on the escape behavior, formation of winged aphids and the number of individuals of Lycium barbarum aphids were analyzed by one-way ANOVA of DPS; Chi-square test was performed for significance of difference.

[0040] (1) Effects of different concentrations of verbenol on the escape behavior of Lycium barbarum aphids

[0041] A transparent ecol...

Embodiment 3

[0051] Example 3, Preparation of Microcapsule Sustained Release Agent Containing Marigold Volatile Matter and Evaluation of Its Control Effect on Lycium Barbarum Aphid

[0052] (1) Preparation of microcapsule sustained release agent

[0053] The marigold volatiles p-α-dimethylstyrene, β-cyclohomocitral, verbenol and caryophyllin were respectively included with β-cyclodextrin to prepare marigold volatiles β-cyclic paste Fine clathrate. The preparation process of this clathrate is described as follows:

[0054] Accurately weigh β-cyclodextrin, place it in a glass beaker, add a certain amount of distilled water to prepare a saturated solution; use 3-5 times the amount of absolute ethanol to fully mix the marigold volatiles, and wait until the temperature of the saturated solution reaches When the temperature reaches a fixed temperature, turn on the stirrer, slowly drop in the prepared marigold volatile matter solution while stirring, keep stirring after the drop is completed, a...

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Abstract

The invention discloses application of a marigold volatile matter beta-cyclo-homocitral to prevention and treatment of Chinese wolfberry aphids, the component of the marigold volatile matter is beta-cyclo-homocitral, and the marigold volatile matter is collected from flowers, stems and leaves of marigold or a whole plant of marigold. The invention further provides application of the aphid prevention and control composition containing the marigold volatile matter to prevention and control of Chinese wolfberry aphids. The invention provides a test data support for effectively preventing and controlling the aphid damage of the Chinese wolfberry and developing a new-generation efficient and environment-friendly plant source pesticide, and also provides a scientific basis for further developing and utilizing the marigold.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and relates to an ecological control technology for wolfberry aphids, in particular to the screening of repelling plants capable of preventing and controlling the wolfberry aphids, and the application of using the volatile matter of the repelling plants to prevent and control the wolfberry aphids. Background technique [0002] Aphids are important agricultural pests. They damage more than 50 families and 350 species of field and greenhouse crops, and can transmit more than 100 plant viruses. Lycium barbarum aphids (Aphis sp.), commonly known as honey bugs, putty insects, drought insects, etc., belong to the order Homoptera and are agricultural pests of the family Aphididae. Lycium barbarum aphids harm the flower organs and young fruits of the leaves of Lycium barbarum, making the shoots shrink and the flowers and fruits become smaller, which seriously affects the yield and quality of Lycium...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01N35/02A01N35/06A01N43/20A01N65/12A01P7/04
CPCA01N35/06A01N43/20A01N25/28
Inventor 刘畅王芳何嘉张蓉魏淑花陈宏灏
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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