Field effect transistor type biosensing device for multi-index detection

Abstract

The present disclosure provides a field effect transistor type biosensing device for multi-index detection. The field effect transistor type biosensing device comprises a biosensor array comprising a plurality of rows and columns of field effect transistor sensors, wherein each transistor gate dielectric region is modified with one or more bioprobe molecules for identifying the same target, and biological probe molecules for recognizing different targets are modified on different sensors; and a plurality of microfluidic channels. Different micro-flow channels enable different types of sample solutions to be detected to flow above the sensors at the same time, so that multiple molecules to be detected can be detected through biological probes with different selectivity fixed on different sensors in the array. According to the biosensing device disclosed by the invention, the detection of various to-be-detected molecules can be realized on the same device, for example, the novel coronavirus infection category, the infection course period and the like of a detected person can be detected, and the detection sensitivity can be improved by multi-probe mixed modification. The invention further provides a preparation method of the biosensing device, a biomolecule detection method and a biomolecule detection system.

Description

technical field [0001] The present disclosure relates to a field-effect transistor biosensor device for multi-indicator detection, a preparation method of the field-effect transistor biosensor device for multi-index detection, a biomolecular detection method, and a biomolecular detection system. Background technique [0002] Biosensors use biosensitive materials as recognition elements to identify the measured target, thereby causing some physical or chemical changes. Biosensors can be widely used in many fields such as rapid detection of infectious diseases and genetic diagnosis of diseases. [0003] The detection technology of biosensors includes fluorescence technology, electrochemical technology and so on. However, the usual detection techniques are complex and require labeling, and rapid detection without labeling in situ cannot be achieved. For example, the principle of PCR requires labeling and tracking of PCR products through fluorescent dyes or fluorescently label...

AI Technical Summary

Problems solved by technology

Electrochemical modification requires additional electrochemical modification steps, which cannot achieve rapid and regional modification, and will cause pollution; there are special requirements for the type of modified material, which is not universal

[0006] In addition, there are still problems such as low detection sensitivity in the existing detection equipment or devices.

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