High-quality and high-yield cultivation method for bletilla striata seedlings

A cultivation method and seedling technology are applied in the field of high-quality and high-yield cultivation of Bletilla striata seedlings, which can solve problems such as low survival rate of Bletilla striata seedlings, and achieve the effects of expanding production scale and improving quality and yield.

Active Publication Date: 2021-06-04
冯耀文
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a high-quality and high-yield cultivation method of bletilla striata seedlings, which can solve the problem of low survival rate of bletilla striata seedlings and simultaneously realize the high-quality of bletilla striata seedlings high yield

Method used

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  • High-quality and high-yield cultivation method for bletilla striata seedlings
  • High-quality and high-yield cultivation method for bletilla striata seedlings
  • High-quality and high-yield cultivation method for bletilla striata seedlings

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Embodiment 1

[0042] The present embodiment provides a high-quality and high-yield cultivation method of bletilla striata seedlings, comprising the following steps:

[0043] (1) Tissue culture

[0044] (1.1) Primary culture

[0045] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium It is: 50% MS basic medium and 1.0mg / L 6-BA.

[0046] (1.2) Proliferation culture

[0047]Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 25°C until the protocorms are completely differentiated into clustered buds, and then the c...

Embodiment 2

[0082] (1) Tissue culture

[0083] (1.1) Primary culture

[0084] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium It is: 50% MS basic medium and 1.0mg / L 6-BA.

[0085] (1.2) Proliferation culture

[0086] Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 26°C until the protocorms are completely differentiated into clustered buds, and then the clustered buds are placed in In the new proliferation medium, repeat culture for 2 generations, each generation 40d; wherein, the formulation of the prol...

Embodiment 3

[0119] The present embodiment provides a high-quality and high-yield cultivation method of bletilla striata seedlings, comprising the following steps:,

[0120] (1) Tissue culture

[0121] (1.1) Primary culture

[0122] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium For: 50% MS basic medium and 1.0mg / L 6-BA;

[0123] (1.2) Proliferation culture

[0124] Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 25°C until the protocorms are completely differentiated into clustered buds, and then the c...

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Abstract

The invention discloses a high-quality and high-yield cultivation method for bletilla striata seedlings. The method comprises the following steps of (1) tissue culture, specifically, primary culture, multiplication culture and rooting and seedling strengthening culture; (2) hardening the tissue culture seedlings, specifically, treating a matrix, selecting bottle seedlings, hardening the seedlings in a bottle and hardening the seedlings in a seedbed; and (3) field planting. Bletilla striata seeds serve as explants, tissue culture seedlings are cultured, different culture media are replaced in different periods to adapt to the growth condition of bletilla striata, the pollution rate of bletilla striata is reduced, the cost is reduced, meanwhile, the quality and yield of bletilla striata seedlings are improved, and comprehensive popularization and research can be carried out.

Description

technical field [0001] The invention relates to the technical field of planting Chinese medicinal materials, in particular to a method for cultivating bletilla striata seedlings with high quality and high yield. Background technique [0002] Bletilla striata is a ground herbaceous plant of Orchidaceae and Bletilla striata. It has multiple values, one is ornamental value: the flowers of Bletilla striata are more beautiful, they can bloom in a dark environment, they can be planted outdoors, or they can be planted in pots, and they are more suitable for flower arrangement. 2. Medicinal value: The tubers of Bletilla striata have many functions such as disinfection, hemostasis and prevention of wound infection, and the effect of sterilization and anti-cancer is also relatively good. Three industrial values: Bletilla striata can also play a positive role in the production of paste, because the viscosity of Bletilla striata is relatively good, so this is an important basis for pas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C21/00A01G22/63A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01H4/008A01H4/001A01G31/00A01G24/28A01G24/15A01G24/12A01G22/63A01C21/005
Inventor 冯耀文
Owner 冯耀文
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