High-quality and high-yield cultivation method for bletilla striata seedlings
A cultivation method and seedling technology are applied in the field of high-quality and high-yield cultivation of Bletilla striata seedlings, which can solve problems such as low survival rate of Bletilla striata seedlings, and achieve the effects of expanding production scale and improving quality and yield.
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Embodiment 1
[0042] The present embodiment provides a high-quality and high-yield cultivation method of bletilla striata seedlings, comprising the following steps:
[0043] (1) Tissue culture
[0044] (1.1) Primary culture
[0045] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium It is: 50% MS basic medium and 1.0mg / L 6-BA.
[0046] (1.2) Proliferation culture
[0047]Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 25°C until the protocorms are completely differentiated into clustered buds, and then the c...
Embodiment 2
[0082] (1) Tissue culture
[0083] (1.1) Primary culture
[0084] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium It is: 50% MS basic medium and 1.0mg / L 6-BA.
[0085] (1.2) Proliferation culture
[0086] Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 26°C until the protocorms are completely differentiated into clustered buds, and then the clustered buds are placed in In the new proliferation medium, repeat culture for 2 generations, each generation 40d; wherein, the formulation of the prol...
Embodiment 3
[0119] The present embodiment provides a high-quality and high-yield cultivation method of bletilla striata seedlings, comprising the following steps:,
[0120] (1) Tissue culture
[0121] (1.1) Primary culture
[0122] After the mature capsules of Bletilla striata were sterilized, the seeds were cut and taken, and the seeds were inoculated on the primary culture medium, cultured in the dark for 20 days, and then moved to the culture room for light culture until the seeds germinated to produce protocorms or clustered buds; wherein, the formula of the primary culture medium For: 50% MS basic medium and 1.0mg / L 6-BA;
[0123] (1.2) Proliferation culture
[0124] Inoculate the protocorms or clustered buds produced by the primary culture into the proliferation medium, and culture and proliferate at a constant temperature under the conditions of light intensity of 20001x and temperature of 25°C until the protocorms are completely differentiated into clustered buds, and then the c...
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