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HTF liquid drop for improving fertilization ability of mouse sperms and preparation method and use method of HTF liquid drop

A fertilization ability, mouse technology, applied in the direction of biochemical equipment and methods, germ cells, microorganisms, etc., can solve the problem of low success rate, achieve the effect of simple use and improve fertilization ability

Active Publication Date: 2021-06-04
NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the overall success rate of ART is currently low, and it is still necessary to optimize its routine operation steps to improve its success rate

Method used

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  • HTF liquid drop for improving fertilization ability of mouse sperms and preparation method and use method of HTF liquid drop
  • HTF liquid drop for improving fertilization ability of mouse sperms and preparation method and use method of HTF liquid drop
  • HTF liquid drop for improving fertilization ability of mouse sperms and preparation method and use method of HTF liquid drop

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Embodiment 1

[0028] The HTF droplet for improving the fertilization ability of mouse sperm according to the present invention contains 1-2 μg of mouse SERPINA5 artificial protein in every 1 mL of HTF droplet.

[0029] As a further definition of the HTF droplet, preferably, each 1 mL HTF droplet contains 1 μg of mouse SERPINA5 artificial protein.

[0030] like figure 1 As shown, the HTF droplet preparation method for promoting the fertilization ability of mouse sperm of the present invention comprises the following steps:

[0031] Step 1.1, make 200 μL / drop of HTF droplets in a petri dish, cover the HTF droplets with tissue paraffin oil, and then put them in the incubator to balance;

[0032] In step 1.2, the mouse SERPINA5 artificial protein powder was formulated with serum-free HTF to form a mouse SERPINA5 artificial protein concentration, and the concentration of the mouse SERPINA5 artificial protein concentration was 240-260 μg / mL;

[0033] In step 1.3, a concentrated mouse SERPINA5 a...

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Abstract

The invention discloses an HTF liquid drop for improving fertilization ability of mouse sperms and a preparation method and a use method of the HTF liquid drop. The preparation method comprises the following steps that 200 [mu]L / drop HTF liquid drop is prepared in a culture dish, tissue paraffin oil covers the HTF liquid drop, and the HTF liquid drop is put in an incubator for balancing; the mouse SERPINA5 artificially synthesized protein powder is prepared into a mouse SERPINA5 artificially synthesized protein concentrated storage by using serum-free HTF, and the concentration of the mouse SERPINA5 artificially synthesized protein concentrated storage is 240-260 [mu]g / mL; and the mouse SERPINA5 artificially synthesized protein concentrated storage is added into the HTF liquid drop, so that the concentration of the mouse SERPINA5 artificially synthesized protein in the HTF liquid drop is 1-2 [mu]g / mL. According to the HTF liquid drop for improving the fertilization capacity of the mouse sperms and the preparation method and use method of the HTF liquid drop, the in-vitro fertilization capacity of the mouse sperms can be effectively improved, and a reference method is provided for optimizing the sperm quality in an ART sperm in-vitro capacitation process.

Description

technical field [0001] The invention relates to the field of sperm capacitation in vitro, in particular to an HTF droplet for improving the fertilization ability of mouse sperm and methods for its preparation and use. Background technique [0002] Couples who have normal sex without any contraceptive measures for more than one year but fail to conceive are called infertility. Among them, the infertility caused by the female factor is called infertility, and the infertility caused by the male factor is called infertility. . At present, the incidence of infertility in my country has reached 15%, and in developed countries, the average incidence of infertility is close to 20%, and the age of infertile couples has tended to be younger. The World Health Organization believes that infertility will become another major killer that threatens human health. It brings a lot of psychological and physical harm to both husband and wife, and seriously affects the happiness index of the fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2501/998
Inventor 姚兵曹思原赵山美子靖俊吴蓉花张光云
Owner NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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