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Method for detecting total flavonoids of ampelopsis grossedentata leaf substitute tea

A technique of displaying snake grape leaves and detection methods, which is applied in the detection field, can solve the problems of no comparability between detection data and no total flavonoids, etc., and achieve reliable detection standards and stable test data

Pending Publication Date: 2021-06-04
湘西土家族苗族自治州食品药品检验所
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Problems solved by technology

Therefore, there is no corresponding uniform detection method for the total flavonoids in the substitute tea of ​​grape leaves of the snake tooth snake (ie, berry tea, vine tea). Reagents perform color reaction, such as the detection wavelength, etc., because there is no unified detection method, so there is no comparability between various detection data

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  • Method for detecting total flavonoids of ampelopsis grossedentata leaf substitute tea
  • Method for detecting total flavonoids of ampelopsis grossedentata leaf substitute tea
  • Method for detecting total flavonoids of ampelopsis grossedentata leaf substitute tea

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Embodiment Construction

[0025] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0026] The method for detecting the total flavonoids of the toothed snake grape leaf substitute tea realized by the present invention comprises the following steps:

[0027] Step 1, sample pretreatment,

[0028] Weigh an appropriate amount of dry Snake-toothed grape leaf substitute tea powder sample, infiltrate and mix with 70% ethanol solution, and then perform ultrasonic extraction in a water bath to obtain an extract;

[0029] The ultrasonic extraction time in the water bath is 0.5 h, the temperature is 50-60° C., the extraction is repeated 2-3 times with 70% ethanol solution, and the extracts are combined. For the det...

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Abstract

The invention relates to a method for detecting total flavonoids of ampelopsis grossedentata leaf substitute tea. The method comprises the following steps: step 1, pre-treating a sample; 2, carrying out standard series and sample detection; 3, measuring absorbance, and measuring an absorbance value after a chromogenic reaction; 4, calculating a result. According to the method, the content of the total flavonoids in the ampelopsis grossedentata leaf substitutional tea is measured by the dihydromyricetin; the method is good in reproducibility, wherein the total flavonoids in the ampelopsis grossedentata leaf substitutional tea can be effectively and comprehensively tested and verified, so that a complete, comprehensive and scientific detection method is formed.

Description

technical field [0001] The present invention relates to the technical field of detection technology, specifically a method for detecting total flavonoids (calculated as dihydromyricetin) on the substitute tea (ie, raspberry tea and vine tea) of the snake-toothed grape leaves. Background technique [0002] Ampelopsis grossedentata leaf substitute tea (i.e. berry tea, vine tea) is Ampelopsis grossedentata (Latin name Ampelopsis grossedentata) of the genus Ampelopsis grossedentata in the grape family. Rockberry, fairy grass, etc. Snake-toothed grape is a kind of wild vine, and its edible parts are leaves (tender leaves and tender stems). Wild Snake-toothed grape is widely distributed in Hunan, Hubei, Guizhou, Jiangxi, Guangxi and other provinces in my country. The substitute tea product of snake-toothed grape leaves is mainly a substitute tea processed from fresh leaves of artificially planted snake-toothed grapes as food raw materials. On December 24, 2013, the former Nationa...

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31G01N2021/775
Inventor 张学英王承江章发盛杨永斌黄静
Owner 湘西土家族苗族自治州食品药品检验所
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