Compositions and methods for treating the eye
A composition and eye technology, applied in drug combination, drug delivery, pharmaceutical formulation, etc., can solve problems such as short residence time
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[0154] The preparation of Pichia anomaly extract A comprises the following steps:
[0155] - culturing the Saccharomyces genus Pichia anomalies in a medium suitable for its development,
[0156] - recovery of biomass by centrifugation,
[0157] - dissolution of biomass,
[0158] - enzymatic hydrolysis at alkaline pH,
[0159] - separation of soluble and insoluble phases,
[0160] - heat treatment,
[0161] - filter, and
[0162] - Sterile filtered.
[0163] II. Characterization of Pichia anomaly Extract A:
[0164] The Pichia anomaly extract A obtained above is characterized by:
[0165] -- a solids content between 48 g / l and 84 g / l,
[0166] -- a pH between 4 and 9,
[0167] -- protein content between 19g / l and 48g / l, and
[0168] - Total sugar content between 10 g / l and 42 g / l.
[0169] 2. Pichia anomaly Extract B
[0170] I. Extraction of Pichia anomaly Extract B:
[0171] The preparation of Pichia anomaly extract B comprises the following steps:
[0172] - cu...
Embodiment 1
[0272] To reduce blockages that may be caused by tight junction structures (i.e., those surface structures that normally inhibit chemical penetration into tissues [e.g., membranes or membrane barriers]), fermented extracts (Hyalurodine) containing The growth medium (i.e., the "treated" medium) contacts the corneal tissue such that the bottom cell layer of the corneal tissue (i.e., the cell layer without tight junction structures that inhibit chemical penetration into the tissue) is immersed in the growth medium, thereby Mucin secretion was observed to be induced in human corneal epithelial adventitia 3D tissue. By contacting the bottom cell layer of the corneal tissue with a growth medium containing a fermented extract of Pichia anomala, the extract can directly contact and move up into the intracorneal tissue without having to first pass through any tight junctions Structural or other surface barriers, thereby improving the bioavailability of Pichia anomala into corneal tissu...
Embodiment 2
[0277] 3D tissues of human corneal epithelium treated with Pichia anomalies ferment extract (Hyalurodine) containing 0.39 mg / mL, 0.78 mg / mL, 1.56 mg / mL and 1.95 mg / mL concentrations in growth medium showed that human corneal epithelium Increased MUC4 gene expression in 3D tissues. *, p<0.05.
[0278] Human corneal epithelial 3D tissue was purchased from MatTek (Ashland, MA, USA). Upon receipt of the human corneal epithelial 3D tissue, the tissue was incubated overnight in MatTek assay medium following the manufacturer's instructions. Human corneal epithelial 3D tissues were divided into five treatment groups of three tissues each. Pichia anomala (Pichia anomala) fermented extract (Hyalurodine) was added to the medium containing human corneal tissue in four of the treatment groups, respectively, to produce 0.39mg / mL, 0.78mg / mL, 1.56mg / mL and a medium concentration of 1.95 mg / mL. Corneal epithelial tissues in all five treatment groups were incubated for two days. Pichia ano...
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