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Biosensor and manufacturing method therefor

A biosensor and substrate technology, applied in biological testing, biological material analysis, instruments, etc., can solve the problems of reduced detection sensitivity, unevenness, uneven surface treatment, etc.

Pending Publication Date: 2021-06-11
LG CHEM LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the liquid phase method has the advantage of being easy to carry out, but it has the disadvantages that the surface treatment of the substrate is somewhat uneven and the processed substance has a low density.
Therefore, there is a problem that the density of the antibody immobilized in the substrate is low and uneven, thereby reducing the detection sensitivity of the target substance of the biosensor

Method used

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  • Biosensor and manufacturing method therefor
  • Biosensor and manufacturing method therefor
  • Biosensor and manufacturing method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0083] Forming materials for biosensors

[0084] Base material: A glass substrate (8 cm x 2 cm) (Sewon Tech, soda lime glass) was used as the base material.

[0085] Support layer forming material: (3-aminopropyl)trimethoxysilane (APTMS) (Sigma-Aldrich, 281778) was used.

[0086] Linking intermediate: p-phenylene diisocyanate (Sigma-Aldrich, 258555) was used.

[0087] Primary antibody: Anti-human IgE antibody (ThermoFisher Scientific, A18797) was used.

[0088] Fabrication of Biosensors

[0089] Form the support layer on the base material:

[0090] Safely place glass substrates such as figure 1 In the reaction chamber of the vapor deposition apparatus configured as shown in , the second valve was opened to form the reaction chamber in a vacuum atmosphere, and the temperature of the reaction chamber was maintained at about 100° C. using the second heat source. Further, (3-aminopropyl)trimethoxysilane (APTMS) as a support layer forming material was charged into the reac...

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Abstract

The present application relates to a biosensor with improved sensitivity in detecting a target substance. The present application also relates to a method for manufacturing a biosensor with improved detection sensitivity. The present application also relates to a method for detecting a target substance by using the biosensor.

Description

technical field [0001] This application claims the benefit of priority based on Korean Patent Application No. 10-2018-0147292 filed on Nov. 26, 2018, the disclosure of which is incorporated herein by reference in its entirety. [0002] This application relates to biosensors and methods for making the same. Background technique [0003] Immunoassays are assays based on antigen-antibody binding reactions. A representative form of immunoassay method is Enzyme-linked immunosorbent assay (ELISA), which is the most commonly used immunodiagnostic method. [0004] Enzyme-linked immunosorbent assays typically detect target substances by immobilizing antibodies in surface-treated substrates. On the other hand, as a surface treatment method of a substrate, a liquid phase method is used in which the substrate is immersed in a solution containing a substance to be treated on the surface. However, the liquid phase method has the advantage of being easy to carry out, however it has the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54393G01N2610/00G01N33/54366G01N33/552G01N33/52
Inventor 吴承埈慎恒范朴焄安俊基
Owner LG CHEM LTD