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A kind of bilirubin-jph203 nanoparticle and its preparation and application

One-JPH203, JPH203 technology, applied in bilirubin-JPH203 nano-particles and its preparation and application field, can solve the problems of bilirubin and JPH203, etc., achieve enhanced drug treatment effect, simple preparation process, and increase half-life in vivo Effect

Active Publication Date: 2022-06-21
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the combined application of bilirubin and JPH203 in the treatment of autoimmune diseases

Method used

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  • A kind of bilirubin-jph203 nanoparticle and its preparation and application
  • A kind of bilirubin-jph203 nanoparticle and its preparation and application
  • A kind of bilirubin-jph203 nanoparticle and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of bilirubin nanoparticles

[0037] According to Table 1, a certain amount of bilirubin and JPH203 were jointly dissolved in 2 mL of dimethyl sulfoxide to form an organic phase, and then the organic phase was slowly dropped into the water phase and stirred continuously for 12 hours; sample. The experimental observations during the preparation were recorded.

[0038] Table 1 Preparation points of bilirubin nanoparticles

[0039]

[0040]

[0041] The bilirubin-JPH203 nanoparticles prepared by the present invention are spherical or worm-shaped, and between 160 and 200 nm, indicating that bilirubin and JPH203 can successfully prepare bimolecular self-assembled nanoparticles under suitable drug ratio and suitable preparation conditions. The experimental preparation method is reasonable and simple.

Embodiment 2

[0042] Example 2 The therapeutic effect of bilirubin-JPH203 nanoparticles on psoriasis in mice

[0043] Psoriasis-like skin lesions were induced on the back skin of ICR mice by applying imiquimod cream continuously for 7 days, and a mouse model of psoriasis was established. On the 8th day of the experiment, the psoriasis mice were observed to have symptoms such as redness, swelling, scales, etc. Different drugs were applied to the back skin of the corresponding groups of mice, and gauze was applied to fix them with bandages. 2 times a day for 8 consecutive days.

[0044] The dosage of each group is as follows:

[0045] Group A: saline group

[0046] Group B: Bilirubin nanoparticles 1 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 8 mg / kg)

[0047] Group C: Bilirubin nanoparticles 2 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 6 mg / kg)

[0048] Group D: Bilirubin nanoparticles 3 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 4 mg / kg)...

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PUM

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Abstract

The invention discloses a bilirubin-JPH203 nano particle and its preparation and application. In the invention, the bilirubin and JPH203 are co-dissolved in an organic solvent, and then the prepared bilirubin solution is dropped into a buffered aqueous solution, stirred and mixed, and the organic solvent is removed to obtain bilirubin nanoparticles. The invention is formed by the self-assembly of two small molecules, the composition ratio is determined, the stability is good, and the two drugs can synergistically play an immune regulation role, which has a good clinical application prospect, and proposes a carrier-free nano-delivery drug system The new methods and approaches can meet the urgent clinical needs of high-end preparations for the treatment of autoimmune diseases.

Description

technical field [0001] The invention belongs to a pharmaceutical nano-particle in the technical field of nano-medicine and its preparation and application, in particular to a bilirubin-JPH203 nano-particle and its preparation and application. Background technique [0002] Autoimmune disease refers to a type of chronic disease caused by the body's immune response to self-antigens, overproduction of autoantibodies or autoreactive T cells, and responses to normal tissues. The commonly used drugs for the treatment of such diseases mainly include non-steroidal anti-inflammatory drugs, steroidal anti-inflammatory drugs and disease-modifying anti-rheumatic drugs. However, although these drugs can effectively alleviate the clinical symptoms of patients, they can only temporarily control the activity and progression of the disease, and are accompanied by serious adverse drug reactions. Common autoimmune diseases include rheumatoid arthritis, psoriasis, and ulcerative colitis. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K31/409A61K31/423A61P1/04A61P17/06A61P19/02A61P29/00A61P37/02
CPCA61K31/423A61K31/409A61K9/14A61P17/06A61P19/02A61P29/00A61P1/04A61P37/02A61K2300/00
Inventor 寇龙发姚情陈瑞杰鲍仕慧蒋欣宇夏星
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE