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Bilirubin-JPH203 nanoparticles as well as preparation and application thereof

One-JPH203, JPH203 technology, applied in the field of bilirubin-JPH203 nanoparticles and its preparation and application, can solve the problems of bilirubin and JPH203, and achieve enhanced drug treatment effect, strong operability and industrialization Foreground good effect

Active Publication Date: 2021-06-18
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the combined application of bilirubin and JPH203 in the treatment of autoimmune diseases

Method used

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  • Bilirubin-JPH203 nanoparticles as well as preparation and application thereof
  • Bilirubin-JPH203 nanoparticles as well as preparation and application thereof
  • Bilirubin-JPH203 nanoparticles as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 bilirubin nanoparticles

[0037] According to Table 1, a certain amount of bilirubin and JPH203 were dissolved in 2mL dimethyl sulfoxide to form an organic phase, and then the organic phase was slowly dropped into the water phase, and the stirring was continued for 12 hours; after dialysis to remove the organic solvent, the obtained sample. Record the experimental observations during the preparation.

[0038] Table 1 Preparation points of bilirubin nanoparticles

[0039]

[0040]

[0041] The bilirubin-JPH203 nanoparticles prepared by the present invention are spherical or worm-like, and are between 160 and 200 nm, indicating that under the appropriate drug ratio and suitable preparation conditions, bimolecular self-assembled nanoparticles can be successfully prepared by bilirubin and JPH203. Granules, the experimental preparation method is reasonable and simple.

Embodiment 2

[0042] Example 2 Therapeutic effect of bilirubin-JPH203 nanoparticles on psoriasis in mice

[0043] Psoriasis-like lesions were induced on the back skin of ICR mice by applying imiquimod cream continuously for 7 days, and a mouse psoriasis model was established. On the 8th day of the experiment, symptoms such as redness, swelling, and scales were observed in the psoriatic mice. Different drugs were applied to the back skin of the mice in the corresponding group, and gauze was applied and fixed with a bandage. 2 times a day for 8 consecutive days.

[0044] The administration of each group is as follows:

[0045] Group A: normal saline group

[0046] Group B: bilirubin nanoparticles 1 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 8 mg / kg)

[0047] Group C: bilirubin nanoparticles 2 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 6 mg / kg)

[0048] Group D: bilirubin nanoparticles 3 prepared in Example 1 (bilirubin dose 10 mg / kg, JPH203 dose 4 mg / kg) ...

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PUM

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Abstract

The invention discloses bilirubin-JPH203 nanoparticles as well as preparation and application thereof. The preparation comprises the following steps of firstly, co-dissolving bilirubin and JPH203 in an organic solvent, then dropwise adding the prepared bilirubin solution into a buffer aqueous solution, stirring and mixing, and removing the organic solvent to obtain the bilirubin nanoparticles. The bilirubin-JPH203 nanoparticles are formed by self-assembly of two micro-molecules, the component proportion is determined, the stability is good, two drugs can synergistically exert the immune regulation effect, the nanoparticles have good clinical application prospects, a new mode and approach for developing a carrier-free nano-delivery drug system are provided, and the urgent demand on high-end preparations for treating autoimmune diseases clinically can be met.

Description

technical field [0001] The invention belongs to a pharmaceutical nanoparticle and its preparation and application in the technical field of nanomedicine, and in particular relates to a bilirubin-JPH203 nanoparticle and its preparation and application. Background technique [0002] Autoimmune disease refers to a type of chronic disease caused by the body's immune response to self-antigens, excessive production of autoantibodies or self-reactive T cells, and response to normal tissues. Clinically commonly used drugs for the treatment of such diseases mainly include non-steroidal anti-inflammatory drugs, steroidal anti-inflammatory drugs, and disease-modifying anti-rheumatic drugs. However, although these drugs can effectively alleviate the clinical symptoms of patients, they can only temporarily control the activity and progress of the disease, and are accompanied by serious adverse drug reactions. Common autoimmune diseases include rheumatoid arthritis, psoriasis, and ulcera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/409A61K31/423A61P1/04A61P17/06A61P19/02A61P29/00A61P37/02
CPCA61K31/423A61K31/409A61K9/14A61P17/06A61P19/02A61P29/00A61P1/04A61P37/02A61K2300/00
Inventor 寇龙发姚情陈瑞杰鲍仕慧蒋欣宇夏星
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE