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Animal experiment operation method for improving antibody secretion of novel coronavirus vaccine to special crowds

An animal experiment, special population technology, applied in the direction of antibody medical components, biochemical equipment and methods, virus antigen components, etc., can solve the problems of uncertain treatment effect of critically ill patients, poor vaccine responsiveness, etc., to improve the immune clearance of characteristic viruses , the effect of reducing the incidence

Pending Publication Date: 2021-06-18
河南道特尔医药科技股份有限公司
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  • Application Information

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Problems solved by technology

[0003] At present, the new crown virus epidemic has not been completely controlled, and there are still many unclear factors. Problems such as virus mutation, poor vaccine reactivity of the elderly, and uncertain treatment effects of critically ill patients are all urgent problems that need to be studied and overcome.

Method used

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  • Animal experiment operation method for improving antibody secretion of novel coronavirus vaccine to special crowds
  • Animal experiment operation method for improving antibody secretion of novel coronavirus vaccine to special crowds
  • Animal experiment operation method for improving antibody secretion of novel coronavirus vaccine to special crowds

Examples

Experimental program
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Effect test

Embodiment 1

[0026] An animal experimental operation method for improving the secretion of new crown vaccine antibodies to special populations, characterized in that it comprises the following steps:

[0027] 1) 30 rats, weighing 250-300g, half female and half male, were stratified and randomly divided into 3 groups according to body weight;

[0028] 2) The 3 groups were 10 rats in the antibody-positive template group (normal immunization), 10 rats in the cell induction group (autologous cell induction group), and 10 rats in the negative control group (non-induced);

[0029] 3) Rats in the antibody-positive template group are routinely injected with the new crown vaccine, and the injection dose of the new crown vaccine (vero inactivated vaccine) is 0.07ug / Kg, and the antibody titer>1 / 1000 is used as the induction template;

[0030] 4) Peripheral blood lymphocytes were separated from the white mice in the cell induction group, and then autologously reinfused after induction based on the ind...

experiment example

[0034] On the fifth day of culturing in the culture medium containing the new coronavirus vaccine 2ng / ml, the titer of the new coronavirus inactivated protein in the supernatant was detected. The in vitro induction group showed a negative result after 1 / 8 dilution, and the non-in vitro induction group was transferred at 1 / 64. Negative, see Table 1. On the 10th day of the culture medium containing the new coronavirus vaccine 4ng / ml, the titer of the new coronavirus inactivated protein in the supernatant was detected. In the in vitro induction group, negative holes appeared at 1 / 32 times dilution, and completely turned negative at 1 / 64, while In the non-in vitro induction group, there is no negative turning hole at this dilution, see Table 2.

[0035] Table 1. Viral antigen titers in the supernatant containing 2ng / ml of the new coronavirus vaccine on the fifth day of culture

[0036]

[0037] Table 2. Viral antigen titers in supernatants containing 4ng / ml of the new coronavi...

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Abstract

The invention relates to the technical field of regulation of secretion of virus specific antibodies, and discloses 30 rats with the weight of 250-300g, half female rats and half male rats, the rats are randomly divided into three groups in a layered manner according to the weight, the three groups are subjected to experimental operation respectively, and sample collection and detection are performed after re-culture. According to an animal experiment operation method for improving antibody secretion of a novel coronavirus vaccine to special crowds, autologous lymphocytes are subjected to in-vitro induction and then are fed back; data can be obtained in an experiment to show that the antibody level of the autologous feedback of the in-vitro induced lymphocytes is in jumping increase; the experimental data can show that the operation method can overcome the defect that the elderly and the weak and sick people respond slowly or do not respond to vaccines; the specific virus immune clearance effect is improved; therefore, the occurrence rate of severe cases is reduced; and the method has a certain treatment effect on the severe cases.

Description

technical field [0001] The invention relates to the technical field of virus-specific antibody secretion regulation, in particular to an animal experimental operation method for improving the antibody secretion of a new crown vaccine to a special population. Background technique [0002] With the development of molecular biology and immunology technology, the targeted function of certain cells can be directed by using reasonable cell culture and induction technology. We used the patient's own peripheral blood lymphocytes in the regulation of hepatitis B antibody-secreting cells, and achieved success by taking hepatitis B antibody-positive healthy people as the regulation standard. [0003] At present, the new crown virus epidemic has not been fully controlled, and there are still many unclear factors. Issues such as virus mutation, poor vaccine reactivity of the elderly, and uncertain treatment effects of critically ill patients are all urgent problems that need to be studie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61P31/14A61K49/00
CPCA61K39/12A61P31/14A61K49/0008A61K2039/5252C12N2770/20034
Inventor 张海鹰
Owner 河南道特尔医药科技股份有限公司
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