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Method for establishing animal extrahepatic biliary tract malignant stenosis model

A method for establishing an animal liver, which is applied in the field of establishing a malignant stenosis model of the extrahepatic biliary tract in animals, can solve the problems of low tumor formation rate and difficulty in producing obstructive jaundice in animal models.

Inactive Publication Date: 2021-07-06
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of model has the disadvantages of low tumor formation rate in animal models, and it is difficult to produce a state with obstructive jaundice after tumor formation in animals.

Method used

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  • Method for establishing animal extrahepatic biliary tract malignant stenosis model
  • Method for establishing animal extrahepatic biliary tract malignant stenosis model
  • Method for establishing animal extrahepatic biliary tract malignant stenosis model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] S1. Isolate from a donor rabbit with a tumor to obtain a tumor tissue mass, specifically:

[0062] The experimental animals were anesthetized through the ear vein, and the limbs of the experimental animals were fixed on the operating table in the supine position, the skin was prepared in the groin, and the towel was disinfected, and the rabbit tumor cells VX2 or tumor tissue were inoculated subcutaneously in the groin of the rabbit (the inoculation amount was 10 5 Suspension of tumor cells or more, 1mm 3 Tissue mass), about 2-3 weeks after inoculation, the tumor tissue in the groin area can grow to a diameter of about 1.5-20cm. Tumors were isolated and dissected under sterile conditions. The vigorously growing fish-like tissue at the edge of the tumor was cut out, placed in a plate filled with normal saline, the necrotic part, fiber, fat and other connective tissues were removed, and the remaining tumor tissue was trimmed into a strip-shaped tumor tissue with a size of...

Embodiment 2

[0066] S1. Isolate from a donor rabbit with a tumor to obtain a tumor tissue mass, specifically:

[0067] The experimental animals were anesthetized through the marginal ear vein, the limbs of the experimental animals were fixed on the operating table in the supine position, the skin was prepared in the groin area, and the towel was sterilized, and the rabbit tumor cells VX2 tumor cells or tumor tissues were inoculated subcutaneously in the groin area of ​​the rabbits (the inoculation amount was 10 5 Suspension of tumor cells or more, 1mm 3 The above tissue mass), about 2-3 weeks after inoculation, the tumor tissue in the groin area can grow to a diameter of about 2cm. Tumors were isolated and dissected under sterile conditions. Cut out the vigorously growing fish-like tissue at the edge of the tumor, place it in a plate filled with normal saline, remove the necrotic part and connective tissue such as fiber and fat, and trim the remaining tumor tissue into a strip-shaped tum...

Embodiment 3

[0071] S1. Isolate from a donor rabbit with a tumor to obtain a tumor tissue mass, specifically:

[0072] The experimental animals were anesthetized through the marginal ear vein, the limbs of the experimental animals were fixed on the operating table in the supine position, the skin was prepared in the groin area, and the towel was sterilized, and the rabbit tumor cells VX2 tumor cells or tumor tissues were inoculated subcutaneously in the groin area of ​​the rabbits (the inoculation amount was 10 5 Suspension of tumor cells or more or 1mm 3 The above tissue mass), about 2-3 weeks after inoculation, the tumor tissue in the groin area can grow to a diameter of about 2cm. Tumors were isolated and dissected under sterile conditions. Cut out the vigorously growing fish-like tissue at the edge of the tumor, place it in a plate filled with normal saline, remove the necrotic part and connective tissue such as fiber and fat, and trim the remaining tumor tissue into a strip-shaped t...

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Abstract

The invention discloses a method for establishing an animal extrahepatic biliary tract malignant stenosis model. The method comprises the following steps that a tumor tissue block mass is obtained, and the tumor tissue block mass is cut to obtain a strip-shaped tumor tissue; an abdominal cavity of an acceptor animal is opened and an extrahepatic bile duct are separated and exposed; and the strip-shaped tumor tissue and a strip-shaped fixing piece are respectively arranged in parallel with a common bile duct in the extrahepatic bile duct and are bound, then the strip-shaped fixing piece is pulled out, the abdominal cavity of the acceptor animal is sutured, disinfected and bound, the strip-shaped tumor tissue grows until the bile duct of the acceptor animal is narrow or blocked to form obstructive jaundice, and the animal extrahepatic biliary tract malignant stenosis model is obtained. The tumor tissue is fixed to the bile duct in parallel, a tumor grows to invade the bile duct, and a bile duct tumor is formed; and when the tumor tissue is bound, the strip-shaped fixing piece is used for protecting and keeping the bile duct unobstructed, and the situation that follow-up bile duct stenosis is caused by tumor invasion is guaranteed. The method is simple and easy to implement and high in success rate, and obstructive jaundice caused by bile duct tumors in reality can be completely simulated.

Description

technical field [0001] The invention relates to the technical field of animal models, in particular to a method for establishing an animal extrahepatic biliary malignant stricture model. Background technique [0002] Malignant stricture of extrahepatic biliary tract is a clinical multiple disease, the most common causes are cholangiocarcinoma and pancreatic cancer, others include primary liver cancer, metastatic tumor, ampullary carcinoma, etc. Malignant stricture of the extrahepatic biliary tract can lead to biliary obstruction, which further leads to a series of symptoms, signs and complications such as jaundice, skin itching, and impaired liver function. Timely relief of obstruction and treatment of tumors invading the bile duct are one of the keys to improving the survival time and quality of life of patients. Interventional diagnosis and treatment such as percutaneous transhepatic biliary drainage, biliary stent implantation, and ablation of biliary tract tumors are im...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 陈斌林润黄勇慧戴海涛唐可禺杨建勇
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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