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Ac18-23 knockout baculovirus expression vector

A technology for expressing vectors and baculoviruses, which is applied in the direction of viruses/bacteriophages, double-stranded DNA viruses, vectors, etc., and can solve the problems of low capacity and low expression of foreign DNA fragments

Inactive Publication Date: 2021-07-06
陕西杆粒生物科技有限公司
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Problems solved by technology

[0016] The purpose of the present invention is to provide a gene knockout baculovirus vector, aiming to solve the problems of low expression and low capacity of foreign DNA fragments mentioned in the background technology

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  • Ac18-23 knockout baculovirus expression vector
  • Ac18-23 knockout baculovirus expression vector
  • Ac18-23 knockout baculovirus expression vector

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0031] 1. Knockout of Ac18-23 gene.

[0032] The knockout strategy is detailed in the appendix figure 1 . Use the primers with the 54bp homology arms upstream and downstream of the Ac18-23 segment, and use the prpsL-AMP plasmid (Journal of Northwest A&F University-Natural Science Edition, December 2015, Volume 43, pages 181-190) as a template, and use The rpsL-AMP expression cassette with the Ac18-23 homology arm was amplified by PCR technique. The primer sequences used in this ste...

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Abstract

The invention discloses a gene knockout type baculovirus expression vector. The vector significantly improves the expression level of foreign proteins by simultaneously knocking out adjacent baculovirus non-essential genes Ac18, Ac19, Ac20, Ac21, Ac22 and Ac23. Meanwhile, the proliferation characteristics of viruses have not been significantly changed. The improvement of the expression yield can reduce the production cost of enterprises, and the knockout of large-segment genes can increase the vector capacity. The baculovirus expression vector can be used in the field of biological product industry, especially in the field of subunit vaccine industry.

Description

technical field [0001] The invention belongs to the technical field of recombinant protein expression, in particular to a gene knockout baculovirus expression vector. Background technique [0002] Baculovirus is a double-stranded DNA virus that specifically infects arthropods. Autographa californica nucleopolyhedrovirus (AcMNPV) is the type species of baculovirus. Since Smith GE and others first expressed human β-interferon gene in insect cells with baculovirus in 1983 (Mol Cell Biol. 1983; 3:2156-65.), due to its low cost, high yield, and various translation Post-modification system and other advantages, the baculovirus expression vector system is widely used in scientific research and production. [0003] However, compared with the prokaryotic expression systems (Escherichia coli, Bacillus subtilis) and yeast expression systems commonly used in industry, the yield of the baculovirus expression vector system is not satisfactory. To this end, people have adopted a variety ...

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Application Information

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IPC IPC(8): C12N15/866C12N15/67C12N15/65
CPCC12N15/65C12N15/86C12N2710/14143C12N2800/105
Inventor 不公告发明人
Owner 陕西杆粒生物科技有限公司
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