Yak bone peptide with hypoglycemic and antioxidative functions and preparation method thereof
A yak bone and anti-oxidation technology, which is applied in the preparation methods of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc. problem, to achieve the effect of convenient industrial production, high food safety, and simple operation
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Embodiment 1
[0043] Embodiment 1 has the preparation method (1) of the yak bone peptide of hypoglycemic and antioxidant function
[0044] This embodiment provides a preparation method of yak bone peptide with hypoglycemic and antioxidant functions, specifically as follows:
[0045] (1) Pretreatment, 200g of yak bone is crushed and cooked at 110°C for 4 hours;
[0046] (2) Two-step enzymatic hydrolysis method, adding 0.3wt% neutral protease accounting for yak bone weight, enzymolysis at 55°C for 2h, adding 0.3wt% compound protease from yak bone to the first step enzymatic hydrolysis reaction product (trypsin is yak Bone 0.2wt% and flavor protease (yak bone 0.1wt%) enzymolysis at 55°C for 2h, 100°C for 15min;
[0047] (3) Ultrafiltration, centrifuging the inactivated peptide hydrolyzate (8000r, 4°C, 15min), the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cut-off of 3000Da or less, to obtain a crude polypeptide solution, which is concentrated and freeze-dr...
Embodiment 2
[0051] Embodiment 2 has the preparation method (2) of the yak bone peptide of hypoglycemic and antioxidant function
[0052] This embodiment provides a preparation method of yak bone peptide with hypoglycemic and antioxidant functions, specifically as follows:
[0053] (1) Pretreatment: 600g of yak bone is crushed and cooked at 120°C for 3.5 hours;
[0054] (2) two-step enzymatic hydrolysis method, add the neutral protease that accounts for 0.4wt% of yak bone weight, enzymolysis 2.2h under 60 ℃, add 0.4wt% compound protease of yak bone (trypsin is Yak bone 0.3wt% and flavor protease (yak bone 0.1wt%) enzymolysis at 60°C for 2h, 100°C for 15min;
[0055] (3) Ultrafiltration: Centrifuge the inactivated peptide hydrolyzate (9000r, 4°C, 15min), and the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cut-off of 2000Da or less to obtain a crude polypeptide solution, which is concentrated and freeze-dried;
[0056] (4) Gel chromatography, the freeze-...
Embodiment 3
[0059] Embodiment 3 has the preparation method (3) of the yak bone peptide of hypoglycemic and antioxidant function
[0060] This embodiment provides a preparation method of yak bone peptide with hypoglycemic and antioxidant functions, specifically as follows:
[0061] (1) Pretreatment, 1000g of yak bone is crushed and cooked at 125°C for 3 hours;
[0062] (2) two-step enzymatic hydrolysis method, add the neutral protease that accounts for 0.5wt% of yak bone weight, enzymolysis 2.2h under 65 ℃, add 0.5wt% compound protease of yak bone (trypsin is Yak bone 0.4wt% and flavor protease (yak bone 0.1wt%) enzymolysis at 60°C for 2h, and 100°C for 15min;
[0063] (3) Ultrafiltration: centrifuge the inactivated peptide hydrolyzate (10000r, 4°C, 20min), and the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cut-off of 2000Da or less to obtain a crude polypeptide solution, which is concentrated and freeze-dried;
[0064] (4) Gel chromatography, the fre...
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