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A preparation method of drug-loaded nanofiber nerve guide

A nerve conduit, drug-loaded nanotechnology, applied in pharmaceutical formulations, drug delivery, medical science and other directions, can solve the problems of acidic degradation products, easy to cause inflammation of surrounding tissue, slow degradation rate, etc., to achieve excellent biocompatibility, The preparation method is simple and the effect of promoting repair

Active Publication Date: 2022-05-27
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polylactic acid (PLA) is a synthetic degradable material, which is non-toxic, strong plasticity, good mechanical properties and biocompatibility, but the degradation rate is slow, and the degradation products are acidic, which is easy to cause damage to surrounding tissues. Inflammation, generally need to compound other materials

Method used

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  • A preparation method of drug-loaded nanofiber nerve guide
  • A preparation method of drug-loaded nanofiber nerve guide
  • A preparation method of drug-loaded nanofiber nerve guide

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A preparation method of a drug-loaded nanofiber nerve conduit, comprising the following steps:

[0033] 1) Preparation of astragalus polysaccharide nanospheres

[0034] Astragalus polysaccharide nanospheres were prepared using chemical cross-linking methods well known in the art. Dissolve 40 mg of chitosan in 20 ml of 11% acetic acid solution, dissolve 10 mg of astragalus polysaccharide in water, and filter with 0.22 μm microporous membrane respectively; under high-speed stirring, add 1 mg / ml of sodium tripolyphosphate solution dropwise to the chitosan In the sugar acetic acid solution (dropping amount of 20ml), continue to stir for 10min after the dropwise addition, then add the above-mentioned astragalus polysaccharide aqueous solution, stir at room temperature overnight, ultracentrifuge (16000r / min, 60min), discard the supernatant, and use 75 % ethanol and distilled water were washed three times each, and the obtained precipitate was frozen at -80° C. for 24 hours, ...

Embodiment 2

[0075] A preparation method of a drug-loaded nanofiber nerve conduit, comprising the following steps:

[0076] 1) Preparation of astragalus polysaccharide nanospheres

[0077] Dissolve 25mg chitosan in 20ml 1% acetic acid solution, dissolve 10mg astragalus polysaccharide in water, and filter with 0.22μm microporous membrane respectively; under high-speed stirring, add 1mg / ml sodium tripolyphosphate solution dropwise to the shell. In the polysaccharide acetic acid solution, continue to stir for 10min after the dropwise addition, then add the above-mentioned astragalus polysaccharide aqueous solution, stir at room temperature overnight, ultracentrifuge, discard the supernatant, wash the precipitate and freeze-dry it into powder for later use.

[0078] 2) Preparation of nerve conduit inner core

[0079] 100 mg of astragalus polysaccharide nanospheres were mixed with 10 g of gelatin, dissolved in water, then put into a special mold, and taken out after freeze-drying to prepare a ...

Embodiment 3

[0085] A preparation method of a drug-loaded nanofiber nerve conduit, comprising the following steps:

[0086] 1) Preparation of astragalus polysaccharide nanospheres

[0087] Dissolve 50 mg of chitosan in 20 ml of 1% acetic acid solution, dissolve 10 mg of astragalus polysaccharide in water, and filter with 0.22 μm microporous membrane respectively; under high-speed stirring, add 1 mg / ml of sodium tripolyphosphate solution dropwise to the shell. In the polysaccharide acetic acid solution, continue to stir for 10min after the dropwise addition, then add the above-mentioned astragalus polysaccharide aqueous solution, stir at room temperature overnight, ultracentrifuge, discard the supernatant, wash the precipitate and freeze-dry it into powder for later use.

[0088] 3) Preparation of nerve conduit inner core

[0089] 10 mg of astragalus polysaccharide nanospheres were mixed with 10 g of gelatin, dissolved in water and then put into a special mold, and taken out after freeze-d...

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Abstract

The invention discloses a preparation method of a drug-loaded nanofiber nerve guide, which comprises the following steps: (1) using chitosan and astragalus polysaccharide as raw materials, sodium tripolyphosphate as a crosslinking agent, and preparing astragalus by a chemical crosslinking method polysaccharide nanospheres; (2) mixing astragalus polysaccharide nanospheres with gelatin solution, and then vacuum freeze-drying to obtain the inner core of the nerve conduit; (3) dissolving gelatin and polylactic acid in hexafluoroisopropanol, and configuring it into a spinning solution, using the electrospinning method to prepare the fiber nerve guide shell; (4) filling the inner core of the nerve guide in the cavity of the fiber nerve guide shell, and then adding it to the genipin solution for cross-linking, after washing Freeze-dried, that is. The nerve conduit prepared by the method has excellent biocompatibility, mechanical properties and degradation properties, can provide porous channels for nerve fiber regeneration, and can slowly release astragalus polysaccharides to further accelerate the nerve repair process, thereby better treating peripheral nerve injuries.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a preparation method of a drug-loaded nanofiber nerve conduit. Background technique [0002] Repair of peripheral nerve injury is a challenging medical problem, and autologous nerve transplantation is the "gold standard" in peripheral nerve repair due to its non-immunogenic effect, but there are also limited supply of donor tissue, neuroma formation Risk, size mismatch, and axonal distribution between the donor nerve and the site of injury. Nerve conduits are artificial tissue-engineered tubes used to bridge nerve stumps as an alternative to autologous nerve grafting. However, the lack of effective biocompatibility and bioactivity is a major disadvantage of most artificial nerve conduits. [0003] Commonly used preparation methods for nerve conduits include casting method, solution dipping method, melt extrusion method, film laying method and electrospinning method. Among t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/00A61L27/18A61L27/20A61L27/22A61L27/50A61L27/54A61L27/56
CPCA61F2/00A61L27/56A61L27/50A61L27/54A61L27/20A61L27/222A61L27/18A61F2002/009A61L2430/32A61L2300/232A61L2300/412A61L2300/602A61L2400/12C08L5/00C08L5/08C08L67/04
Inventor 许沛虎马毓聪徐海星李政林跃辛洋吕忆菲赵凯杰文景杨海霞何怡张晋源高腾飞徐起鼎许润天
Owner WUHAN UNIV OF TECH
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