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A kind of application of turmeric volatile oil

A technology of turmeric volatile oil and turmeric, which is applied in the field of pesticides, can solve the problems of the lethality rate of pine wood nematodes being less than 30%, the inhibition effect of pine wood nematodes is general, and the toxicity is high, so as to achieve good pine wood nematode effects, easy acquisition, and low activity. Effect

Active Publication Date: 2022-01-25
沈阳恩柽研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active ingredients mainly include abamectin, thiazolphos, isofenphos-methyl, methamidophos, methyl bromide, aldicarb, amino-oligosaccharins, triamron, cartap, metamuc, carbofuran, and butylsulfone Carbofuran, etc. Most of the products are organophosphorus products, which are highly toxic. Most of the pesticides have a general inhibitory effect on pine xylophilus parasitic in woody plants such as Pinaceae. The fatality rate of wood nematode is less than 30%
In addition, although there have been many research reports on the research on the biological activity of turmeric, some reports also show that turmeric has certain insecticidal and bacteriostatic activities, but these active studies are basically limited to medicine. The research report is also very simple, especially there is no research report on the anti-pine wood nematode activity of turmeric volatile oil

Method used

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  • A kind of application of turmeric volatile oil
  • A kind of application of turmeric volatile oil
  • A kind of application of turmeric volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The preparation of embodiment 1 turmeric volatile oil

[0089] Wash and crush fresh turmeric, add distilled water with 4 times the mass of turmeric, and beat for 30 minutes; then put the mixed solution in a distillation bottle, connect it to a volatile oil tester, and distill at 100°C for 3 hours; during distillation, the lower end of the volatile oil tester is screwed open, Receive distilled water and volatile oil directly, and use a separating funnel to separate water and oil after distillation. The oily substance is light yellow and has a turmeric smell, which is the volatile oil of turmeric.

Embodiment 2

[0090] Embodiment 2 Turmeric volatile oil anti-pine wood nematode experiment

[0091] Medicament configuration: the turmeric volatile oil is configured into a 10 g / L solution with a DMSO test system (4% DMSO+15% Tween 80), and the DMSO test system (4% DMSO+15% Tween 80) is used as a blank control group.

[0092] Preparation of pine wood nematode suspension: Suspend the pine wood nematode on the corn grain medium with an appropriate amount of sterilized water in the water phase, and then separate the living pine wood nematode suspension by the Bellman funnel method, and the nematode suspension Centrifuge at 2000r / min for 2min, remove the supernatant and keep the precipitate, add appropriate amount of sterilized water, observe the nematode age, activity status and count the number of nematodes, and finally use sterilized water to quantify the nematode suspension to 1000 nematodes / mL. Most of the nematodes are in the J3 stage, and a few are in the J2 and J4 stages. Reserve at 4°C...

Embodiment 3

[0097] Embodiment 3 turmeric volatile oil anti-meloidogyne incognita experiment

[0098] Medicament configuration: the turmeric volatile oil is configured into a 20g / L solution with a DMSO test system (4% DMSO+15% Tween 80), and the DMSO test system (4% DMSO+15% Tween 80) is used as a blank control group.

[0099] Insect source for testing: Take the roots of potted tomatoes (about 60 days after inoculation), wash the floating soil, sterilize in 1% NaClO solution for 3 minutes, and then wash with sterile water. Pick out the root oocysts under a stereoscope, put them into 24-well plates according to the experimental groups, place 5 oocysts in each well, add 250 μL of sterile water, pick out the oocyst shells after 3 days of incubation, and hatch out of the plate. The second instar larvae were used for subsequent experiments.

[0100] Bioassay test: Add 250 μL of control group and treatment group to the wells of the hatched root-knot nematode liquid in the 24-well plate, and mix...

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Abstract

The invention belongs to the technical field of pesticides, and in particular relates to the application of turmeric volatile oil. The application of the turmeric volatile oil against pine wood nematode (Bursaphelenchus xylophilus). Turmeric volatile oil is a volatile oil obtained from turmeric (Curcuma longa L.) through water extraction. The turmeric volatile oil and its extracted active compounds can be used as anti-nematode products or as active ingredients of products to act on pine wood nematodes. The 48h corrected mortality rate exceeds 80%, and significantly reduces the activity of nematodes; the active compound obtained by further processing the turmeric volatile oil and its extraction and other preparations and / or compounds with insecticidal and anti-nematode effects can play a synergistic synergistic effect.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to the application of turmeric volatile oil. Background technique [0002] Nematodes, belonging to the phylum Nematodes, can be found everywhere in fresh water, sea water, and land. It is an animal group second only to insects in diversity. Currently, there are more than 28,000 recorded species, including 256 families and 2,271 genera. There are still a large number of species that have not yet been identified. name. In 1743, after the British scientist Needham first discovered the tritici nematode in diseased wheat grains, different reports of various types of nematodes appeared successively in various regions of the world. At present, there are more than 16,000 species of parasitic nematodes, which are pathogens of animals including many plants and humans, among which the more serious and widespread ones mainly include root-knot nematodes, cyst nematodes and pine w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/48A01P5/00
CPCA01N65/48
Inventor 李世友赵福强李帅强刘刚
Owner 沈阳恩柽研究院有限公司
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