A kind of microemulsion droplets of products after w/o/w type pcr amplification based on microfluidic system and preparation method thereof

Abstract

The invention provides a microemulsion droplet of a W / O / W type PCR amplified product based on a microfluidic system and a preparation method of the microemulsion droplet, the microemulsion droplet includes an amplified W / O type microemulsion droplet , an oil phase A and an outer water phase, wherein the amplified W / O microemulsion droplets include an inner water phase and an oil phase B, prepared by a microfluidic system, and the amplified W / O microemulsion droplets, oil phase A The volume ratio of the inner water phase to the outer water phase is 1:3:1000; the volume ratio of the inner water phase to the oil phase B is 40:3. The present invention provides a preparation method of W / O / W microemulsion droplets. The obtained microemulsion droplets can effectively establish a new type of microemulsion droplet sorting platform, and can effectively solve the problem of tissue The conundrum of cellular heterogeneity that samples cannot crack. In the present invention, by strictly screening the formula ratio and preparation parameters, the average particle diameter is 70 μm, all of which are spherical particles with uniform particle size distribution, and the stability experiment shows that the microemulsion droplets prepared by the present invention can be stored at room temperature, and The particle size changes little in a short time at room temperature.

Description

A kind of microemulsion droplets of products after W / O / W PCR amplification based on microfluidic system and the same Preparation technical field The invention belongs to biotechnology field, relate in particular to a kind of after W / O / W type PCR amplification based on microfluidic system Microemulsion droplets of products and methods for their preparation. Background technique With the establishment and development of single-cell genome sequencing and single-molecule sequencing technology, the analysis of cell genome characteristics The analysis went to the single-cell and single-molecule level. Single-cell genomic resolution not only enables researchers to Analyzing the heterogeneity of tumor cells also enables genomic studies of rare cells that have traditionally been difficult to detect. Depend on Since the size of cells is generally in the micrometer scale, microfluidic chip microdroplet technology has been widely used in cell sample processing. use. Cells...

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Problems solved by technology

It is well known that the stability of W / O droplets is relatively poor. Therefore, most amplified biological samples cannot be preserved intact for other experimental studies, which is undoubtedly a waste of scientific research.

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