Unlock instant, AI-driven research and patent intelligence for your innovation.

Fermentation pichia pastoris RS-13 and application thereof

A technology for fermenting Pichia pastoris and RS-13, which is applied to fermenting Pichia pastoris RS-13 and its application fields, can solve the problems of low utilization rate, serious bean dregs and beany smell, and limited product development, and achieve good overall flavor Effect

Pending Publication Date: 2021-07-23
SHANGHAI INST OF TECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: the bean dregs has a low utilization rate and limited product development due to the severe beany smell

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fermentation pichia pastoris RS-13 and application thereof
  • Fermentation pichia pastoris RS-13 and application thereof
  • Fermentation pichia pastoris RS-13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening of a fermented Pichia pastoris RS-13 that can reduce the beany smell of bean dregs:

[0042] (1) Collection of samples: Naturally fermented milk fans purchased from farmer's markets in Dali and Jianchuan, Yunnan, were transported to the laboratory in a low-temperature sampling box and stored at 4°C.

[0043] (2) Separation and purification: each sample was mashed with a mortar, and serially diluted with sterile water. Draw 0.1mL10 -3 、10 -4 、10 -5 、10 -6 The dilutions of four g / mL concentration gradients were spread in the PDA medium, and three replicates were set for each dilution. Place the plate in a constant temperature incubator at 30±1°C and incubate it upside down for 48-60 hours. Use a sterile toothpick to pick colonies of different sizes, colors, and shapes and streak them repeatedly on the PDA medium to separate and purify them to obtain a single colony.

[0044] A total of 30 yeast strains of different colors, shapes, and sizes were isolated fr...

Embodiment 2

[0058] The target bacterial strain (RS-13) that embodiment 1 screens gained carries out microbiological identification:

[0059] (1) Colony characteristics: The target strain (RS-13) was isolated by streaking on a PDA medium plate, and cultured aerobically at 30° C. for 48 hours, and the strain grew well. Its colony is round, porcelain white, raised, smooth, moist and sticky, easy to be provoked, such as figure 1 shown.

[0060] (2) Cell characteristics: the cells are rod-shaped, arranged in chains of different lengths and lengths, and also in a single scattered arrangement. The cell size is generally 0.8μm×2.0μm, does not produce spores, figure 2 shown.

[0061] (3) Cultural characteristics: the minimum growth temperature of the target strain is 15°C, the maximum growth temperature is 42°C, and the best growth temperature is 28-37°C; the maximum and minimum initial growth pH are 7.0 and 4.0, and the optimum growth initial pH is 6.8.

[0062] (4) Genetics characteristics:...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses fermentation pichia pastoris RS-13 and an application thereof. The preservation number of the fermentation pichia pastoris RS-13 is CCTCC NO: M 2020964, the Latin name of the fermentation pichia pastoris RS-13 is Pichia fermentarian RS-13, and the fermentation pichia pastoris RS-13 is preserved in the China Center for Type Culture Collection on December 24, 2020. Tests prove that after the fermentation pichia pastoris is used for fermenting bean dregs, the beany flavor in the bean dregs can be reduced to a level imperceptible to a human body, and the content of beany flavor compounds is reduced to 1 / 40 of that of unfermented bean dregs. The fermentation pichia pastoris RS-13 is used in a bean dreg beverage product, and the bean dreg beverage without beany flavor can be prepared, so that the technical problems of low utilization rate, limited development of related products and the like caused by serious beany flavor of the bean dregs can be effectively solved, and the fermentation pichia pastoris RS-13 has a good application prospect.

Description

technical field [0001] The invention relates to fermenting Pichia pastoris RS-13 and application thereof, belonging to the technical field of food fermentation. Background technique [0002] Okara is the main by-product in the process of producing soybean milk or tofu from soybeans. my country is currently one of the countries with the largest production of okara in the world, with an annual production capacity of 20 million tons of wet okara. Bean dregs, as a kind of dietary fiber and protein resource with high quality, low price and wide sources, have good development and application prospects. However, there are many problems that reduce its utilization rate, among which the severe beany smell is one of the main influencing factors. [0003] Beany smell is a common smell in leguminous plants, and it is also one of the main factors that limit the wide application of bean foods. It is not caused by a specific substance, but a combination of several or even dozens of flavor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23L11/30A23L11/50A23L11/65C12R1/84
CPCA23L11/37A23L2/382Y02P60/87
Inventor 田怀香何晓葳陈臣于海燕陈霜刘政郭伟邹灵刘瑶
Owner SHANGHAI INST OF TECH