Perishable waste degrading microbial agent and application thereof

A technology of garbage degradation and bacterial agent, applied in the directions of fixing on or in inorganic carriers, fixing on/in organic carriers, biochemical equipment and methods, etc., can solve the problem of low treatment efficiency, pollution and treatment costs High cost and other problems, to achieve the effect of simple and easy operation, low cost and prolonged storage time

Active Publication Date: 2021-07-23
HANGZHOU NAXI ECOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of perishable waste mainly includes landfill method, incineration method, composting method and high-temperature anaerobic digestion method. The above-mentioned methods all have defects such as serious pollution, low treatment efficiency and high treatment cost.

Method used

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  • Perishable waste degrading microbial agent and application thereof
  • Perishable waste degrading microbial agent and application thereof
  • Perishable waste degrading microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] This embodiment provides a perishable garbage-degrading bacterial agent, and the specific preparation method includes the following steps.

[0090] Step 1, preparation of sulfobutyl ether-β-cyclodextrin: basis figure 1 In the process, 0.01mol of recrystallized β-cyclodextrin is dissolved in 500g of 12% sodium hydroxide solution, dispersed until colorless and transparent, and 0.15mol of 1,4-sulfobutyl lactone is taken at a rate of 2mL / min Slowly drop into the above solution, at 60°C, stir and react at 600r / min for 18h, and maintain the pH of the reaction solution at 11.5 (with 0.5mol / L sodium hydroxide solution and / or 0.5mol / L hydrochloric acid, the same below), When the reaction solution turns yellow and clear, adjust the pH to neutral and concentrate to 1 / 3 volume, within 1 hour, slowly drop into an ethanol solution with a volume content of not less than 85vol% to form a precipitate, filter and dry the precipitate at 45°C for 12 hours , re-precipitate with absolute et...

Embodiment 2

[0096] This embodiment provides a perishable garbage-degrading bacterial agent, and the specific preparation method includes the following steps.

[0097] Step 1. Preparation of sulfobutyl ether-β-cyclodextrin: Dissolve 0.01mol of recrystallized β-cyclodextrin in 500g of 15% sodium hydroxide solution, disperse until colorless and transparent, and take 0.18mol of 1,4- Slowly drop sulfobutyl lactone into the above solution at a rate of 4mL / min, stir and react at 750r / min at 65°C for 12h, maintain the pH of the reaction solution at 11.8, adjust the pH to neutral when the reaction solution turns yellow and clear And concentrate to 1 / 2 volume, slowly drop into ethanol solution with a volume content of 90vol% to form a precipitate, filter and dry the precipitate at 55°C for 9 hours, and re-precipitate with absolute ethanol, and dry in a vacuum oven at 55°C until constant Weight is obtained, the schematic diagram of the hydrogen spectrum is as follows figure 2 shown.

[0098] Ste...

Embodiment 3

[0103] This embodiment provides a perishable garbage-degrading bacterial agent, and the specific preparation method includes the following steps.

[0104] Step 1. Preparation of sulfobutyl ether-β-cyclodextrin: Dissolve 0.01mol of recrystallized β-cyclodextrin in 500g of 12% sodium hydroxide solution, disperse until colorless and transparent, and take 0.20mol of 1,4- The sulfobutyl lactone was slowly dropped into the above solution at a rate of 2 mL / min. At 65° C., the reaction was stirred at 600 r / min for 12 h to maintain the pH of the reaction solution at 11.5 (with 0.5 mol / L sodium hydroxide solution and / or 0.5mol / L hydrochloric acid, the same below), when the reaction solution turns yellow and clear, adjust the pH to neutral and concentrate to 1 / 3 volume, slowly drop into the ethanol solution with a volume content of not less than 85vol% to form a precipitate, filter The precipitate was dried at 45° C. for 12 hours, reprecipitated with absolute ethanol, and dried in a vacu...

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Abstract

The invention relates to the technical field of perishable waste treatment, and particularly relates to a perishable waste degrading microbial agent and application thereof. The perishable waste degrading microbial agent comprises basic microbes, synergistic microbes, cross-linked slurry and gypsum powder, wherein the cross-linked slurry comprises a water solution of sodium alginate and high-substitution-degree sulfobutyl ether-beta-cyclodextrin in a weight ratio of (0.5-5): 1, and the gypsum powder contains 25-40% of phosphogypsum whiskers modified by silane coupling agents. The composition of basic microbes and synergistic microbes is adjusted and optimized, and the perishable waste degrading microbial agent is obtained by compounding the composition with the cross-linked slurry, adding modified gypsum powder and carrying out granulation; the obtained immobilized microorganisms are viable bacteria, so that there is no need to activate the immobilized microorganisms when in use, and the immobilized microorganisms can be stored for 12 months or above at normal temperature; and the perishable waste degrading microbial agent can finally degrade perishable waste into carbon dioxide and water in combination with an immobilized enzyme preparation, so that the reduction rate of perishable waste is up to 99% or above.

Description

technical field [0001] The invention relates to the technical field of perishable garbage treatment, in particular to a perishable garbage degrading bacterial agent, in particular to a perishable garbage degrading bacterial agent and its application. Background technique [0002] According to the statistics of the Ministry of Housing and Urban-Rural Development, my country's annual domestic waste production is more than 400 million tons, and it is growing at an average annual rate of 10%, ranking first in the world. According to statistics from the Hangzhou Urban Management Committee, the total amount of domestic waste generated in Hangzhou in 2019 is about 12,000 tons per day. The terminal treatment methods of these domestic wastes are incineration and landfill, which not only cannot meet the increasing demand for domestic waste disposal, but also It will also seriously endanger air resources and groundwater resources. The biogas and leachate produced by anaerobic consumpti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C08B37/16B09B3/00C12R1/125C12R1/07C12R1/085C12R1/38C12R1/02
CPCC12N11/14C12N11/10C08B37/0012B09B3/00
Inventor 徐坚麟付源王俊滔
Owner HANGZHOU NAXI ECOLOGICAL TECH CO LTD
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