Method for efficiently preparing livestock and poultry cartilage collagen polypeptide

A collagen polypeptide and cartilage technology, applied in the biological field, can solve problems such as unsatisfactory extraction efficiency of collagen polypeptide in livestock and poultry cartilage, and achieve the effect of improving application value and extraction efficiency

Active Publication Date: 2021-07-23
内蒙古蒙肽生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For this reason, the technical problem to be solved by the present invention is to provide a method for efficiently preparing collagen polypeptides of livestock and poultry cartilage, so as to solve the problem of unsatisfactory extraction efficiency of collagen polypeptides of livestock and poultry cartilage in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The method for efficiently preparing livestock and poultry cartilage collagen polypeptide described in this embodiment comprises the following steps:

[0031] (1) Get 1kg of the precipitate processed according to the aforementioned impurity removal and pretreatment steps, and set aside;

[0032] (2) Add 0.5mol / L pepsin solution (acetic acid pH 3.0) to the cartilage after the pretreatment according to the ratio of solid to liquid of 1:10 times, place it in a pressure extraction device, and control the enzymolysis temperature to At 4°C, the enzymatic hydrolysis treatment was carried out alternately under the control of -0.1MPa micro-negative pressure environment and normal pressure environment. After the solution was completed, centrifuge at 4°C and 12000rpm for 30 minutes, collect the supernatant, and add 20% sodium chloride solution for overnight salting out, then centrifuge at 4°C and 12000rpm to collect flocculent white precipitate;

[0033] (3) Dissolve the obtaine...

Embodiment 2

[0036] The method for efficiently preparing livestock and poultry cartilage collagen polypeptide described in this embodiment comprises the following steps:

[0037] (1) Get 1kg of the precipitate processed according to the aforementioned impurity removal and pretreatment steps, and set aside;

[0038] (2) Add 0.3mol / L pepsin solution (acetic acid pH 3.0) to the pretreated cartilage according to the ratio of solid to liquid of 1:10 times, place it in a pressure extraction device, and control the enzymolysis temperature to At 4°C, the enzymatic hydrolysis treatment was carried out alternately in a micro-negative pressure environment of -0.1MPa and a normal pressure environment. After the solution, centrifuge at 4°C and 12000rpm for 30 minutes, collect the supernatant, add 20% sodium chloride solution for overnight salting out, and then centrifuge at 4°C and 12000rpm to collect flocculent white precipitate;

[0039] (3) Dissolve the obtained precipitate in 0.5mol / L acetic acid...

Embodiment 3

[0042] The method for efficiently preparing livestock and poultry cartilage collagen polypeptide described in this embodiment comprises the following steps:

[0043] (1) Get 1kg of the precipitate processed according to the aforementioned impurity removal and pretreatment steps, and set aside;

[0044] (2) Add 0.8mol / L pepsin solution (acetic acid pH 3.0) to the pretreated cartilage according to the ratio of solid to liquid of 1:10 times, place it in a pressure extraction device, and control the enzymolysis temperature to At 4°C, the enzymatic hydrolysis treatment was carried out alternately under the control of -0.1MPa micro-negative pressure environment and normal pressure environment. After the solution was completed, centrifuge at 4°C and 12000rpm for 30 minutes, collect the supernatant, and add 20% sodium chloride solution for overnight salting out, then centrifuge at 4°C and 12000rpm to collect flocculent white precipitate;

[0045] (3) Dissolve the obtained precipitat...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a method for efficiently preparing livestock and poultry cartilage collagen polypeptide. According to the preparation method of the livestock and poultry cartilage collagen, on the basis of a traditional cartilage collagen preparation method, the extraction efficiency of collagen can be effectively improved by further adjusting and controlling the extraction pressure in the enzymolysis process and alternately performing micro negative pressure extraction and normal pressure extraction, and the application value of cartilage waste is further improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for efficiently preparing livestock and poultry cartilage collagen polypeptides. Background technique [0002] my country is a large animal husbandry country, especially with the rapid development of livestock and poultry breeding, the by-products have increased significantly. The deep processing of these by-products must keep up with the development of the aquaculture industry in order to improve the economic benefits of livestock and poultry breeding and reduce waste of resources. Promote the sustainable, rapid, healthy and stable development of the industry. Therefore, it is of great significance to strengthen the development and utilization of livestock and poultry by-products. The development and utilization of traditional livestock and poultry by-products mainly include the comprehensive processing and utilization of livestock and poultry blood, bones, visc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/30C07K1/16
CPCC12P21/06C07K14/78
Inventor 温宇旗徐杨陈丽娜魏鹏飞
Owner 内蒙古蒙肽生物工程有限公司
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