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Method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification

A technology based on morphology and molecular identification, applied in the field of plant tissue identification, can solve problems such as difficult to distinguish rubber grass

Pending Publication Date: 2021-07-23
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is difficult to distinguish rubber grass from dandelion by simply comparing the molecular weight

Method used

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  • Method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification
  • Method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification
  • Method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification

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Embodiment Construction

[0038] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0039] Such as figure 1 As shown, the following steps are included in this implementation example:

[0040] A selects the complete plant tissue of rubber grass to be tested, and collects plant leaves, flower buds, rhizomes and seeds respectively;

[0041] B selects the rubber grass to be tested to carry out the primary screening of morphological observation to obtain the rubber grass once screened;

[0042] Both the common dandelion and rubber grass belong to the genus Dandelion and at first appear to be the same species. Rubber grass and common dandelions have rosette-like leaves. The leaves of the common dandelion are pinnate but those of the rubber grass are more wedge-shaped. Common dandelion leaves have ragged margins and a wedge-shaped base. The tip ...

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Abstract

The invention discloses a method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification. The method comprises the following steps: selecting complete plant tissues of taraxacum kok-saghyz rodin to be detected; selecting the taraxacum kok-saghyz rodin to be detected for morphological observation and conducting primary screening to obtain primarily screened taraxacum kok-saghyz rodin; conducting molecular identification on the screened taraxacum kok-saghyz rodin, carrying out statistics and analysis on the obtained data, outputting results; and identifying the taraxacum kok-saghyz rodin to be detected according to the results. According to the method of the present invention, morphological observation and molecular identification can be combined to eliminate interference from herba taraxaci and taraxacum kok-saghyz rodin hybrids. The method of the present invention is suitable for collection from plant materials of field taraxacum kok-saghyz rodin and can also be used for preservation of pure germplasm of taraxacum kok-saghyz rodin.

Description

technical field [0001] The invention belongs to the technical field of plant tissue identification, in particular to a method for identifying rubber grass based on morphological and molecular identification. Background technique [0002] Rubber grass, where the mevalonate (MVA) pathway is used to regulate natural rubber biosynthesis, rubber grass is diploid (2n=16) with a relatively simple genome of 1.29 Gb in length containing 46,731 predicted proteins Encoding gene, rubber grass root contains high molecular weight rubber molecules, its characteristics are comparable to the rubber latex of Hevea brasiliensis, can be used as an alternative rubber crop, rubber grass root has a high rubber content, which accounts for 3% to 28% of its total dry weight[17] , they are also a new source of inulin (linear β-(2-1)-linked fructans), another abundant dandelion metabolite stored in parenchymal root cell vacuoles near the phloem, separated from the ectoplasm Adjacent to latex insects, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6858G06K9/62
CPCC12Q1/6858G06V10/751
Inventor 谢全亮王旭初李鸿彬
Owner SHIHEZI UNIVERSITY
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