33 results about "Taraxacum kok-saghyz" patented technology

The invention relates to an interplanting method of kok-saghyz in an orchard, which includes steps of selecting the orchard of which tree age is not more than 5 years, the soil layer is deep and nutrition is sufficient; applying fully decomposed organic fertilizer and phosphorus diamine; preparing soil; ploughing to great depth in proper time; after deep ploughing 25cm-30cm, timely raking; ridgingalong a fruit tree line direction, and paving a drip irrigation zone in every ridge, then leveling the ridge face and covering a film; lifting seedlings and transplanting in mid-to-late days of May;then performing water irrigation, topdressing, pest control and weeding on the orchard; harvesting roots and stems of the kok-saghyz in the first ten-day period of October in the next year. The interplanting method of kok-saghyz in the orchard has the advantages of effectively making up the technical blank of interplanting the kok-saghyz in the orchard, improving the land utilization rate of the orchard, increasing the economic benefit of land area, reducing the planting cost, improving the output and quality of kok-saghyz, and increasing output and income. Besides, the planting method is simple in operation, easy to popularize, and extensive in application value.

The invention provides the genetically manipulated herbicide-resistant rubber producing dandelion plants and seed of said plants. Another aspect of the invention comprises progeny plants, or seeds, or regenerable parts of plants and seeds of the genetically manipulated herbicide-resistant dandelion plants. Applicants have further found that use of root cells for transformation and other optimized protocols enable quick transformation with high plant regeneration. Further, Applicants have developed the first transformation / regeneration protocol that is successful without the addition of hormone treatment.

Provided herein are organic solvent-based processes for the isolation of purified natural rubber from non-Hevea plants. The processes are particularly useful with plant matter that comprises at least 90% by weight roots sourced from Taraxacum kok-saghyz (Russian dandelion), Scorzonera tau-saghyz (Tau-Saghyz), Scorzonera uzbekistanica, and combinations thereof. Also provided herein is a purified non-Hevea rubber product containing 100 phr of rubber sourced from Taraxacum kok-saghyz, Scorzonera tau-saghyz, Scorzonera uzbekistanica, and combinations thereof, having a specified purity level. Additionally provided are sulfur-vulcanizable rubber compounds and sulfur-vulcanized rubber compounds that incorporate 10-100 phr of purified non-Hevea rubber product sourced from Taraxacum kok-saghyz, Scorzonera tau-saghyz, Scorzonera uzbekistanica, and combinations thereof, having a specified purity level.

The invention relates to a method for regenerating plants with Taraxacum kok-saghyz Rodin leaves. The method comprises steps as follows: the pretreated Taraxacum kok-saghyz Rodin leaves are sequentially subjected to callus induction, callus subculture, adventitious bud induction and rooting culture. With the adoption of the induction method, the propagation coefficient is high, browned and albino seedlings are avoided, the regenerated plants are robust, root systems are well developed, the transplantation survival rate is high, and a large number of regenerated plants can be obtained within a short time.

The invention relates to a Taraxacum kok-saghyz inulin degrading enzyme gene Tk1-FEH and application thereof. The sequence of the Taraxacum kok-saghyz inulin degrading enzyme Tk1-FEH is shown in SEQ ID NO: 1. According to the invention, Tk1-FEH gene is overexpressed and the function of the gene is studied. The results show that endurance of Taraxacum kok-saghyz genetically modified by the Tk1-FEHgene to drought stress is improved significantly, after 38 days of water cut-off, the Taraxacum kok-saghyz can still maintain a good growth state, and the root biomass increases significantly under normal growth state, with the dry root weight increasing by more than 170%, which indicates that the gene can be used in the research of drought resistance and root biomass improvement of Taraxacum kok-saghyz and other crops. The Taraxacum kok-saghyz inulin degrading enzyme gene Tk1-FEH comes from Taraxacum kok-saghyz, does not produce gene drift, has little impact on the environment, and provides new gene resources for improving drought resistance and root biomass of Taraxacum kok-saghyz and other dandelion plants.

The invention provides a testing method of content of rubber in dandelion rubber grass, comprising the following steps: preparing a sample of a standard substance of dandelion rubber, making a standard curve, testing the sample to be tested, and calculating the rubber content. First the standard substance of the dandelion rubber is prepared and a calibration substance after splitting is determined, a pyrolysis temperature of a pyrolysis instrument and a programmed temperature of a gas chromatograph are set, and the standard substance is put into a pyrolysis gas chromatography-mass spectrometerfor sample testing, after the sample is tested, a chromatogram is obtained, and the standard curve is made by using the peak area of the calibration substance; and then the actual sample to be testedis put into a pyrolysis gas chromatograph for measurement, and the peak area of the calibration substance of the actual sample is substituted into the standard curve to acquire the rubber content ofthe actual sample. The testing method provided by the invention has good repeatability and high accuracy, and can be used for rubber content detection of the dandelion rubber grass.

The invention provides a Taraxacum kok saghyz Rodin genetic transformation method. The Taraxacum kok saghyz Rodin genetic transformation method includes the steps that (1) young and tender leaves of Xinjiang Taraxacum kok saghyz Rodin germplasm is taken and subjected to cutting processing, and put in a bud induction medium with MS+1.5-1.6mg / L6-BA+0.1mg / LNAA+20g / L sucrose and pH being 5.8 for culture; after 50 days of culture, the excellent Taraxacum kok saghyz Rodin germplasm suitable for tissue culture is selected; the excellent Taraxacum kok saghyz Rodin germplasm is transferred to a rootingmedium with 1 / 2MS+20g / L sucrose and pH being 5.8 to become a regenerated Taraxacum kok saghyz Rodin tissue culture seedling; (2) the seedling leaves are taken and cut, and pre-cultured on the bud induction medium for 2 days; and (3) an agrobacterium infection solution is added, dip-dyeing is conducted, the dip-dyed material is transferred into the bud induction medium, and co-culture is performedor 3 days in the dark; the co-cultured material is transferred to a bud induction medium containing 400 mg / L Car to be cultured for one week; the cultured material is transferred to a screening medium with MS+1.5-1.6mg / L 6-BA+0.1mg / L NAA+20g / L sucrose+400mg / L Car and 8-10mg / L Hyg and pH being 5.8 to induce regeneration of resistant buds; and the material with resistant buds is transferred to a new rooting medium with 1 / 2MS+20g / L sucrose+400mg / L Car and 8-10mg / L Hyg and pH being 5.8, so that a Taraxacum kok saghyz Rodin resistant regenerated plant is obtained. According to the Taraxacum kok saghyz Rodin genetic transformation method, the induction rate of resistant regenerated buds is greater than 30%, the rate of malformation of transgenic regenerated seedlings is low, and the positive detectable rate of transgenic Taraxacum kok saghyz Rodin is greater than 90%.

The invention discloses a gene enhancing herbicide resistance of rubber plants, a mediating vector containing the gene enhancing the herbicide resistance of the rubber plants and an application of thegene in genetic improvement of the rubber plants. The nucleotide sequence of the gene is shown in SEQ ID NO:1. The gene enhancing the herbicide resistance of the rubber plants is applied to genetic transformation of the rubber plants, and the herbicide resistance of the rubber plants is improved after transformation.

The invention discloses a method for extracting taraxacum rubber. The method comprises the following steps: (1) boiling dried roots of taraxacum kok-saghyz, and removing bark and pulling out cores toseparate root pulp and root bark; (2) boiling the root pulp in a sulfuric acid solution, carrying out filtering, and drying filter residues to obtain rubber, wherein the filtrate is reserved for lateruse; and (3) boiling the root bark in water, and then boiling the root bark in an alkali solution, carrying out filtering, and drying the filter residues to obtain rubber. The method disclosed by theinvention is simple, cost is low, and the taraxacum rubber is free of toxicity. In addition, high-purity rubber can be extracted.

The invention discloses a water-based extraction method for taraxacum rubber. The method comprises the following steps: (1) cleaning dry roots of Taraxacum kok-saghyz, and drying the cleaned roots atroom temperature; (2) preprocessing the roots by a rolling and kneading machine, a rolling tank or a stirring machine, and removing residues to obtain residues containing rubber threads; and (3) adding water into a mixture containing the rubber threads, performing stirring at a high speed, and settling and separating the obtained mixture to obtain the taraxacum rubber. Only water is used for extraction in the whole extraction process, so environmental protection is truly achieved; and water has a low price, so the problem of high cost of a solvent in a traditional solvent extraction method fortaraxacum rubber is solved.

The invention relates to the technical field of molecular markers, and in particular relates to a molecular marker combination for identifying Russian dandelion and application of the molecular markercombination. In the invention, a method for identifying and distinguishing Russian dandelion is designed according to three SSR markers ONT21, ONT876 and ONT15498 in dandelion plant gene, specifically, corresponding specific primers can be designed for the three SSR markers, then samples to be identified are subjected to PCR reaction, and the species type of the material to be identified can be determined through the amplified bands. The specific primers designed by the invention have high specificity and sensitivity, and the accuracy of amplification results can be ensured. The identification method of Russian dandelion based on three SSR markers provided by the invention can accurately identify and distinguish Russian dandelion from various dandelions of the same genus and different species, effectively prevent interspecific hybridization, and has great significance in the breeding and genetic protection of Russian dandelion.

The invention discloses a method for identifying taraxacum kok-saghyz rodin based on morphology and molecular identification. The method comprises the following steps: selecting complete plant tissues of taraxacum kok-saghyz rodin to be detected; selecting the taraxacum kok-saghyz rodin to be detected for morphological observation and conducting primary screening to obtain primarily screened taraxacum kok-saghyz rodin; conducting molecular identification on the screened taraxacum kok-saghyz rodin, carrying out statistics and analysis on the obtained data, outputting results; and identifying the taraxacum kok-saghyz rodin to be detected according to the results. According to the method of the present invention, morphological observation and molecular identification can be combined to eliminate interference from herba taraxaci and taraxacum kok-saghyz rodin hybrids. The method of the present invention is suitable for collection from plant materials of field taraxacum kok-saghyz rodin and can also be used for preservation of pure germplasm of taraxacum kok-saghyz rodin.

The invention aims to provide a fluid-type direct-seeding kok-saghyz planting mode and a related device thereof and belongs to the technical field of plant growing. Distilled water is adopted to soakdry seeds for 7-9h, a water retaining agent, a binder, compound fertilizer and water are mixed according to a mass ratio of 5:5:100:16000, kok-saghyz seeds and mixed liquid are mixed according to a mass ratio of 1:40, and the compound fertilizer is prepared by mixing diammonium phosphate, urea and potassium chloride according to a mass ratio of 4:2:3. Planting process of kok-saghyz is simplified greatly, and the long-lasting difficult problem that conventional planting methods of kok-saghyz are labor-consuming, time-consuming and high in planting cost is solved. The device ensures seeding interval accuracy and planting discharging accuracy, seed and water-fertilizer mixing can be performed continuously, and planting and mulching can be performed at the same time; the device is simple in structure, convenient in maintenance and convenient for agricultural popularization and use and has extensive applicability in the field of kok-saghyz planting.

The invention discloses a method for extracting chloroplast from kok-saghyz leaves. The method comprises the following steps: collecting complete leaves of kok-saghyz plants; crushing the leaf tissues, and performing primary extraction to obtain a kok-saghyz chloroplast crude extract; adding the kok-saghyz chloroplast crude extract into the upper layer of the cell separation liquid, and then carrying out ultra-high-speed centrifugation for the first time at 4 DEG C for 10-15 minutes; after centrifugation is finished, sucking the precipitates of the cell separation liquid, washing the precipitates with chloroplast extracting liquid and then performing second-time ultra-high-speed centrifugation for 12 min, and obtaining the complete kok-saghyz chloroplast through the precipitates. The method can be applied to kok-saghyz chloroplast separation, can also be used for extracting chloroplast of compositae or congeneric plants, is not only suitable for being collected from common plant materials, but also can be used for chloroplast separation of recalcitrant plant leaves.

The invention discloses a main control substrate of communication equipment. The main control substrate comprises the following components in parts by weight: 70-100 parts of a phenolic resin, 2-5 parts of triglycidyl isocyanurate, 1-3 parts of a catalyst, 2-7 parts of an ultraviolet resistant agent, 1-10 parts of polyurethane, 5-20 parts of an epoxy resin, 2-7 parts of titanium dioxide, 3-8 partsof barium sulfate, 2-10 parts of light calcium carbonate, 1-7 parts of orange peel powder, 1-6 parts of green alga powder, 1-5 parts of kieselguhr, 1-10 parts of a pigment, 3-6 parts of aids, 1-2 parts of taraxacum kok-saghyz powder and 1-10 parts of water hyacinth powder. Due to adoption of multiple types of aquatic plant powder, the main control substrate is low in cost, free of pollution, goodin interference resistance and applicable to large-scale popularization, and electromagnetic interference of different elements is reduced.

The invention relates to a nutrient solution for improving the transplanting survival rate of kok-saghyz tissue culture seedlings. The nutrient solution comprises the following components: 412.5 to 825 parts of ammonium nitrate, 475 to 950 parts of potassium nitrate, 110 to 220 parts of calcium chloride dihydrate, 92.5 to 185 parts of magnesium sulfate heptahydrate, 42.5 to 85 parts of potassium dihydrogen phosphate and the like. The prepared nutrient solution is suitable for rooting and growth of kok-saghyz tissue culture seedlings, a seedling hardening device saves space, is low in price, economical and practical, and after transplanting and seedling hardening culture are conducted for 15-30 days through the steps, the final transplanting survival rate reaches 99% or above, and the plant biomass is increased by 50% or above.

The invention relates to a self-degradation rubber greening device. A base is connected to an outer rubber layer; a liquid storage sac and a seed sac are arranged in the outer rubber layer; degradation liquid and plastic nails are arranged in the liquid storage sac; grass seeds and one plastic nail is arranged in the seed sac; a planting outlet is connected to the lower end of the seed sac; the liquid storage sac and the seed sac are arranged in the outer rubber layer and the base. When external force acts on the outer rubber layer, the plastic nails can pierce the liquid storage sac, the degradation liquid can enter the whole outer rubber layer, and the outer rubber layer, the liquid storage sac and the seed sac can automatically degrade, so that the seeds in the seed sac can enter soil through the planting outlet. Thus, the grass seeds can be planted during automatic degradation, and the natural ecology can be recovered.

The invention relates to the field of biological technology, in particular to rubber grass drought tolerance gene TkMYC2, protein, primer, carrier, host bacterium and application thereof. The nucleotide sequence of the TkMYC2 gene cDNA is shown in SEQ ID NO:1. The amino acid sequence of the encoded protein is shown in SEQ ID NO:2. Under natural drought stress, compared with the wild type, the leaves of transgenic rubber grass overexpressing TkMYC2 grew well. Overexpression of TkMYC2 gene can significantly increase POD, SOD, CAT enzyme activity in response to drought stress. The rubber grass TkMYC2 gene of the invention provides a new solution for improving the drought resistance of the rubber grass.

The invention discloses a method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry. According to the method, a pyrolysis gas chromatograph-mass spectrometer is utilized to perform high-flux and high-sensitivity quantification on the natural rubber at the roots of the kok-saghyz. According to the detection method, the pretreatment process is simple, the detection result is accurate, and tedious pretreatment purification steps and a large amount of solvent consumption are not needed. The method can be used for screening the kok-saghyz variety withhigh natural rubber content.

The invention relates to the technical field of plant factory planting, in particular to a light environment regulation and control method for regulating and controlling root system growth and development of kok-saghyz, which is characterized in that a light environment is provided for seedling raising and cultivation through an LED lamp, the light quantum proportion range of red light of 600-699 nm and blue light of 400-499 nm is controlled to be 3.32-5.21, and the proportion range of the sum of light quantum of far red light of 700-780 nm and light quantum of green light of 500-599 nm is 13-18% when the sum of light quantum of far red light of 700-780 nm and light quantum of green light of 500-599 nm is 380-780 nm. The kok-saghyz plant factory cultivation is carried out through the full-artificial light, annual production can be achieved, and meanwhile root system expansion of the kok-saghyz can be promoted.

The invention discloses a method for detecting a breast duct of a root tissue of a kok-saghyz plant by a freezing slide preparation technology. The method comprises the following steps: selecting a complete plant tissue of the kok-saghyz to be detected, and putting into a stationary liquid; performing vacuum air exhaust on the stationary liquid, wherein the air exhaust time is controlled to be 1-3 min; placing at 4 DEG C for 6-8 hours; taking out the plant tissue in the stationary liquid and performing immersion cleaning for three times, wherein each time is 30 min; the plant tissues are dehydrated step by step through a sucrose solution; after the dehydrated plant tissue adsorbs excessive moisture, embedding the dehydrated plant tissue by using a Biosharp embedding agent, placing the plant tissue at -20 DEG C for 30-45 minutes, slicing the root tissue by using a freezing slicer, adding 10% glycerol for sealing, and solidifying and sealing by using the glycerol for 10 minutes to obtain a slide specimen so as to obtain the distribution condition of the breast ducts in the kok-saghyz root tissue. According to the method, the distribution and quantity conditions of the breast ducts in the kok-saghyz roots can be rapidly detected, the detection period is greatly shortened, and the growth conditions of the breast ducts of the kok-saghyz can be intuitively reflected.

The invention relates to the field of plant genetics and breeding, in particular to a method for inducing tetraploid plants of rubber grass. During mutagenesis treatment, the rubber grass seedlings are soaked in 0.04-0.08 mM asulphin solution for 30-60 minutes. Through the optimization of the method for inducing the tetraploid plant of the rubber grass, the present invention greatly improves the mutagenesis rate, reduces the chimera, and significantly improves the seedling rate at the same time. During application, the mutation rate of tetraploid plants can reach more than 10%, and the seedling rate can reach more than 70%, forming a set of efficient and stable induction methods for tetraploid plants of rubber grass.

The invention relates to the technical field of plant gene editing, in particular to a method for site-specific mutagenesis of taraxacum kok-saghyz or dandelion genes by using a CRISPR / Cas9 system. According to the gene site-specific mutagenesis method, a CRISPR / Cas9 based targeting sgRNA sequence is designed for a target gene, multiple sgRNAs can be driven by using one CmYLCV promoter, differenttargeting sgRNAs are constructed at intervals by adopting a 20bp Csy4 hairpin sequence, a CRISPR / Cas9 carrier is transferred into an acceptor material carrying the target gene, and a target gene site-specific mutated mutant plant is obtained. The method provided by the invention greatly improves the efficiency and gene mutation rate of multi-target simultaneous knockout vector construction, has the characteristics of short experimental period, simple operation and the like, lays a technical foundation for gene mutation of taraxacum kok-saghyz and dandelion, and provides a new method for improved breeding of taraxacum kok-saghyz and dandelion.

The invention relates to a method for regenerating plants with Taraxacum kok-saghyz Rodin leaves. The method comprises steps as follows: the pretreated Taraxacum kok-saghyz Rodin leaves are sequentially subjected to callus induction, callus subculture, adventitious bud induction and rooting culture. With the adoption of the induction method, the propagation coefficient is high, browned and albino seedlings are avoided, the regenerated plants are robust, root systems are well developed, the transplantation survival rate is high, and a large number of regenerated plants can be obtained within a short time.

The invention relates to the technical field of rubber grass gene editing, in particular to a method for simultaneously knocking out 1-SST and 1-FFT genes of rubber grass by using a CRISPR / Cas9 system. The sgRNAs specifically targeting the inulin synthase genes 1-SST and 1-FFT provided by the present invention can efficiently guide the endonuclease protein Cas9 to cut at the target site and generate double-stranded DNA breaks, combined with resistance screening and DNA sequencing Accurate and efficient simultaneous knockout of the rubbery inulin synthase genes 1-SST and 1-FFT. The present invention provides a convenient and efficient method for creating a rubber grass inulin synthase gene mutant, which effectively avoids the problems of long period, mutation uncertainty, high screening difficulty and high cost in conventional mutagenesis breeding. Breeding of grass elite germplasm provides a new method.

The invention relates to a method for inducing rooting of hydroponic taraxacum kok-saghyz. According to the method, hormone with a certain concentration is added into an improved Hoagland nutrient solution to serve as a hydroponic nutrient solution, rootless seedlings are fixed to holes of a floating plate, so that plant bases can make full contact with the nutrient solution, and the biomass of the plant roots and leaves is remarkably improved after the seedlings are cultured for a period of time through the nutrient solution. The method for inducing rooting of the hydroponic taraxacum kok-saghyz is simple and easy to implement and low in cost. The taraxacum kok-saghyz plants obtained with the method are robust and grow well, a large number of new hairy roots are formed through induction, and an effective way is provided for rapid growth recovery of the taraxacum kok-saghyz plants after root extraction and improvement of the production efficiency of extracting synanthrin and natural rubber from the taraxacum kok-saghyz.

The invention discloses a taraxacum kok-saghyz seedling culture method and culture device, and aims to solve the problems that current taraxacum kok-saghyz wild growing environments are seriously damaged, so that taraxacum kok-saghyz seeds difficultly germinate, the survival rate of taraxacum kok-saghyz seedlings is not high, plants are weak, and later growth of root systems is affected. By the aid of the special taraxacum kok-saghyz seedling culture device, seed low-temperature treatment and growing environments are artificially simulated, taraxacum kok-saghyz seed germination characteristicsand seedling growth rules are met, a technical method is built and can keep a certain temperature, humidity and illumination condition of soil, effectively improves the growing speed of the taraxacumkok-saghyz seedlings and effectively increase the survival rate of the taraxacum kok-saghyz seedlings, so that growing states of the cultured taraxacum kok-saghyz seedlings are good, the growth and development speed of the taraxacum kok-saghyz seedlings is remarkably improved, leaves are green and have gloss, the root systems are developed, diseases and insect pests are avoided, and the method and the device have wide application popularization values.

The invention discloses a preparation method for welding agents and belongs to the technical field of welding agent preparation. The preparation method for the welding agents comprises the steps that taraxacum kok-saghyz roots are cleaned and mixed with distilled water to be cooked so as to remove impurities, and filter residues are obtained through filtration; the filter residues, cyclohexane and the like are repeatedly ground into thick liquid after being evenly mixed, rubber materials in the thick liquid are extracted, solvents are removed, and welding agent auxiliary materials are obtained for standby application; then aluminum ash is subjected to water separation for purification, the aluminum ash, flinder diamonds and the like are subjected to ball milling and stirring reaction in the acidic condition and under the protection of nitrogen, and welding agent base materials are prepared through filtration; and finally the welding agent base materials, the standby welding agent auxiliary materials and the like are mixed, ground, sieved and dried to obtain the welding agents. According to the preparation method for the welding agents, the preparation steps are easy, the obtained welding agents are high in welding strength, no spatter phenomenon occurs and no toxic gas is released during welding, and the welding agents are high in rust resistance.

The invention discloses a preliminary treatment device for rubber grass. The preliminary treatment device for the rubber grass comprises a stirring cavity arranged in a main machine body. The stirringcavity is internally provided with a stirring device for heating and fully stirring frozen crushed rubber grass. An exhaust cavity communicating with the outside of the main machine body is formed inthe right side of the stirring cavity. A water vapor purification device for discharging hot gas in the stirring cavity and carrying out filtering and purifying is arranged in the exhaust cavity. A semi-finished product liquid cavity is formed in the lower side of the stirring cavity, and an auxiliary tank communicating with the two cavities is arranged between the stirring cavity and the semi-finished product liquid cavity. The preliminary treatment device for the rubber grass is easy and convenient to operate and low in manufacturing cost, mixing and filtering can be achieved, rubber grassjuice can be fully extracted through boiling and is mixed with water, the resource utilization rate is improved, and waste gas is purified and filtered through boiling.