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Method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry

A technology of gas chromatography-mass spectrometry and natural rubber, which is applied in the field of detection of natural rubber in rubber grass by pyrolysis gas chromatography-mass spectrometry, can solve the problems of poor quantitative accuracy of the area integration method, and achieve simple pretreatment process and accurate detection results Effect

Active Publication Date: 2021-02-09
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of Py-GC-MS to the breeding of rubber grass has not been reported, and in other plant applications, due to the large difference in the response of mass spectrometry to different substances, the quantitative accuracy of the widely used area integration method is relatively poor

Method used

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  • Method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry
  • Method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry
  • Method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Analysis of thermal cracking products of natural rubber in rubber grass in gas chromatography mass spectrometry and development of its quantitative method

[0054] 1) Wash rubber grass 1151 and take its root.

[0055] 2) Collect the root of rubber grass, and dry it at 50° C. for 48 hours to constant weight.

[0056] 3) Take liquid nitrogen, place the rubber grass sample dried in step 2 in liquid nitrogen, and grind it into powder.

[0057] 4) Weigh 1 mg of rubber straw sample processed in step 3, and place it in a special steel cup for cracking instrument.

[0058] 5) The cracking process of natural rubber in rubber grass is as follows: figure 1 As shown, high molecular natural rubber is cracked into free radicals at high temperature, and then monomers (isoprene), dimers (limonene), trimers, and tetramers are formed by free radicals.

[0059] 6) The rubber grass root sample in step 4) was cracked in the evolved gas analysis (EGA) mode. The full-scan chrom...

Embodiment 2

[0071] Embodiment 2, high-throughput and high-sensitivity quantitative application of natural rubber in rubber grass

[0072] 1) Wash the 6-month-old root of Rubber Grass 1151, and take the root.

[0073] 2) Collect the roots and dry them at 50° C. for 48 hours to constant weight.

[0074] 3) Take liquid nitrogen, place the rubber grass sample dried in step 2) in liquid nitrogen, and grind it into powder.

[0075] 4) Weigh about 1 mg of the rubber grass sample processed in step 3), and place it in a special steel cup for a pyrolyzer, and set up 3 biological replicates for the sample.

[0076] 5) Dissolve 10 mg of polystyrene in 1 ml of toluene, and add 2 μl of 10 mg / ml polystyrene in toluene to the sample steel cup.

[0077] 6) Place the steel cup on the autosampler of the pyrolysis instrument.

[0078] 7) The gas chromatography-mass spectrometry conditions are as in step 8) of Example 1, and the parameters of the pyrolyzer are as in step 9) of Example 1 in the double-click...

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Abstract

The invention discloses a method for detecting natural rubber in kok-saghyz through pyrolysis gas chromatography-mass spectrometry. According to the method, a pyrolysis gas chromatograph-mass spectrometer is utilized to perform high-flux and high-sensitivity quantification on the natural rubber at the roots of the kok-saghyz. According to the detection method, the pretreatment process is simple, the detection result is accurate, and tedious pretreatment purification steps and a large amount of solvent consumption are not needed. The method can be used for screening the kok-saghyz variety withhigh natural rubber content.

Description

technical field [0001] The invention belongs to the field of analysis, and relates to a method for detecting natural rubber in rubber grass by thermal cracking gas chromatography and mass spectrometry. Background technique [0002] Natural rubber is an important strategic material and industrial raw material. It has good resilience, water resistance, plasticity and insulation. After proper treatment, it can resist acid and alkali, heat, tear and wear, so it is widely used It is used in the production of tires, shoes, medical gloves, tapes and gas masks, etc. Natural rubber, coal, steel, and petroleum are called the four basic industrial raw materials. Although the rise of synthetic rubber has a certain impact on the development of the natural rubber industry, it still cannot shake its status as a basic industrial raw material. At present, rubber tree is the only commercial source of natural rubber, but its growth has special requirements on soil organic matter, temperature...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 王海敬范秀丽徐霞崔霞李家洋
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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