Cosmetic for repairing and relieving skin irritation and preparation method thereof
A technology for skin irritation and cosmetics, applied in cosmetic preparations, cosmetics, preparations for skin care, etc., can solve problems such as skin barrier dysfunction repair, and achieve the effect of restoring skin barrier function, excellent skin beautifying effect, and relieving skin irritation.
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Embodiment 1
[0048] Embodiment 1: preparation method of Monascus fermented product extract
[0049] 1. Fermentation method of Monascus fermented product:
[0050] (1) Liquid seed culture: Weigh about 5g of Daqu powder into a 250mL Erlenmeyer flask, add about 30mL of sterile water, shake for 30 minutes to make a bacterial suspension, take the bacterial suspension and inoculate it in the proliferation medium, at 30°C, Cultivate at 120r / min for 4 days to obtain liquid seed solution.
[0051] (2) Solid-state fermentation: select some glutinous rice, open it evenly, and place it in a constant temperature environment of 50°C for drying. Then weigh 100g and pour it into a conical flask with a volume of 500mL. Select some pure water and put it in a beaker, add lactic acid to it until the pH of the solution is 5.0, pour the solution into a horn bottle until the liquid level is 5cm above the top of the glutinous rice. Soak for 12 hours and gently shake the triangular flask, and visually check tha...
Embodiment 2
[0059] Embodiment 2: preparation method of comfrey extract
[0060] Dried xinjiang comfrey, crushed and sieved to 60 mesh, sterilized and placed in an extraction tank, added ethylhexyl palmitate (10ml / g), left to soak at room temperature for 24 hours, and centrifuged to obtain a comfrey extract.
Embodiment 3
[0062] In vitro experiments were conducted to investigate the effect of red yeast rice extract combined with comfrey extract on the secretion of ceramide, free fatty acid and hyaluronic acid from keratinocyte cell line HEKa cells, so as to evaluate its repairing effect on skin barrier function.
[0063] 1. Test sample processing
[0064] Dissolve comfrey extract in DMSO, then add Monascus ferment extract, and finally add Epilife TM Culture medium, according to the ratio and specific feeding in Table 1 and Table 2, configure different Epilife TM Media mix solution. Among them, there are 7 groups of compositions with different ratios, and 3 groups of comparative examples.
[0065] Table 1 Cell test ratio
[0066]
[0067]
[0068] Table 2 Composition of each component of the cell test
[0069]
[0070] 2. Cell culture
[0071] HEKa cells from normal human skin were treated with Epilife containing 1% HKGS (Human Keratinocyte Growth Supplement) TM Culture medium (co...
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