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A kind of macrophage exosome membrane-coated biomimetic nanoparticle and its preparation method and application

A macrophage and nanoparticle technology, which can be used in pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. The effect of the degree of infiltration

Active Publication Date: 2021-11-12
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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Problems solved by technology

However, with conventional transfection of genes, the expression of genes in exosomes is less, and recent studies have also demonstrated that siRNAs are prone to degradation when electroporation induces siRNAs into exosomes, suggesting the need to establish a more reliable protocol

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  • A kind of macrophage exosome membrane-coated biomimetic nanoparticle and its preparation method and application
  • A kind of macrophage exosome membrane-coated biomimetic nanoparticle and its preparation method and application
  • A kind of macrophage exosome membrane-coated biomimetic nanoparticle and its preparation method and application

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Embodiment Construction

[0032] DESCRIPTION OF DRAWINGS Examples and embodiments of the present invention will be still further embodiment.

[0033] In the present invention, we explored a biomimetic nanoparticles M2 macrophages exosomes film coated (EM-PLGA @ Dnmt3aos smart silencer ), Which consists of PLGA @ Dnmt3aos smart silencer Core and exosomes film. Use EM-PLGA @ Dnmt3aos smart silencer Inhibition AA (allergic asthma) M2 macrophages in significant polarization ( figure 1 ), The biomimetic drug accumulation in the lungs and effectively promoting gene silencing, while reducing the degree of inflammatory cell infiltration of the airways. Therefore, we believe PLGA NP-based exosomes film will contribute to the therapeutic Dnmt3aos smart silencer Delivered to the airways, it is more effective to guide in the treatment of AA mouse model.

[0034] Material and method

[0035] 1.1 Mice

[0036] C57BL / 6 (H-2d) female mice (6-8 weeks, 20-22g) were purchased from Laboratory Animal Center Qinglongshan ...

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Abstract

The invention belongs to the field of biomedicine, and relates to a macrophage exosome membrane-coated biomimetic nanoparticle and a preparation method and application thereof, which are formed by the exosome membrane-coated nanoparticle; The source of M2 macrophages is isolated; the nanoparticles are composed of polylactic-glycolic acid copolymer PLGA and Dnmt3aos smart silencer Co-emulsification is formed. The present invention uses EM‑PLGA@Dnmt3aos smart silencer Inhibition of M2 macrophage polarization in allergic asthma is striking, and the biomimetic effectively accumulates in the lungs and promotes gene silencing, while reducing airway infiltration by inflammatory cells. M2 macrophage exosomal membrane-based PLGA NPs facilitate the incorporation of therapeutic Dnmt3aos smart silencer delivered to the airway to guide its more effective treatment in a mouse model of AA.

Description

Technical field [0001] The present invention is in the biomedical field, it relates to a macrophage exosomes biomimetic membrane coated nanoparticles and a preparation method and application. Background technique [0002] There are 300 million people worldwide suffer from asthma, estimated to affect 400 million by 2025, allergic asthma (AA) accounted for 90% of childhood asthma cases, accounting for 50% of adult asthma cases. Macrophages are the most abundant in the lungs of immune cells (about 70% of all immune cells), they play an important role in airway inflammation caused by environmental allergens in. Adoptive transfer of M2 macrophages may aggravate allergic airway inflammation in mice, it showed M2 polarization of macrophages play an important role during the onset of AA. Therefore, the development of new strategies for M2 macrophages is considered a promising therapeutic AA method. [0003] In our previous study, the differentially expressed in thousands lncRNA M1 / ​​M2...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/34A61K47/59A61K47/69A61K31/7088A61P11/06A61P37/08
CPCA61K9/5153A61K9/5176A61K9/5192A61K31/7088A61K47/59A61K47/6935A61P11/06A61P37/08
Inventor 吕坤裴纬亚张莺莺
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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