Application of alkaloid compound in preparation of product for preventing and/or treating heart injury
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A heart injury, compound technology, applied in the field of alkaloid compounds in the preparation of products for the prevention and/or treatment of heart injury, to achieve the effect of inhibiting cardiomyocyte toxicity
Active Publication Date: 2021-08-06
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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[0005]
In addition, there are still many active substances contained in seabuckthorn that have not been isolated and identified. If we can conduct more in-depth and subtle research on the new chemical c
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Embodiment 1
[0035] Example 1 Inhibitory Effect of Compound I on Cardiomyocyte Apoptosis in H9c2 Myocardial Toxic Model
[0036] 1. Toxic effects of alkaloid compound Ⅰ on H9c2 cardiomyocytes
[0037] After H9c2 cells were incubated with different concentrations (0.1 μM, 1 μM, 5 μM, 10 μM, 20 μM, 40 μM, 80 μM, 160 μM) of alkaloid compound I for 24 h, the toxic effects of alkaloid compounds on cardiomyocytes were evaluated by MTT colorimetry.
[0038] 2. Effect of alkaloid compound Ⅰ on DOX-induced toxicity of H9c2 cardiomyocytes
[0039] (1) Pre-incubate H9c2 cells with alkaloid compound Ⅰ for 1 h, then add 2.5 μM doxorubicin (DOX) and culture for 24 h, use MTT colorimetric method to screen alkaloid compound Ⅰ for DOX-induced cardiomyocyte apoptosis activity, and Determine the optimal concentration range for action.
[0040](2) After treating the H9c2 cardiomyocytes with different seabuckthorn alkaloids for 1 hour, treat them with 2.5 μM DOX for 24 hours, then extract the protein, lyse, ...
Embodiment 2
[0049] Tablets containing Compound I
[0050] The following ingredients are produced for each tablet:
[0051]
[0052] After mixing, granulating, blending and tableting, 250mg tablets were made.
Embodiment 3
[0054] Injection containing compound Ⅰ
[0055] Produce injections with the following components per 1 ml:
[0056]
[0057] The above solid substance was dissolved in purified water, and the solution was aseptically filled into 1 ml ampoules.
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Abstract
The invention discloses an application of an alkaloid compound in preparation of a product for preventing and/or treating heart injury, activity research is performed on the alkaloid compound, and it is verified that the alkaloid compound can inhibit apoptosis induced by myocardial toxicity. According to the present invention, by down-regulating the expression level of p-JNK and/or Cleaved-Caspase-3, the myocardial apoptosis induced by the anthracycline drug DOX is inhibited so as to alleviate the myocardial cytotoxicity effect, achieve the myocardial cytotoxicity prevention and/or treatment effect, and can be used for preparing drugs for preventing and/or treating myocardial injury related diseases.
Description
technical field [0001] The invention relates to the field of medical applications of alkaloid compounds, in particular to the use of alkaloid compounds in the preparation of products for preventing and / or treating heart damage. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a deciduous shrub of the family Echinaceae and the genus Hippophae. It is characterized by drought resistance, wind and sand resistance, and can survive on salinized land, so it is widely used in soil and water conservation. . Seabuckthorn is grown in large quantities in northwest China for desert greening. [0003] Seabuckthorn is rich in nutrients, including active substances such as vitamins, flavonoids, triterpenoids, oils and fatty acids, phenols, volatile oils, trace elements, phospholipids, 5-hydroxytryptamine, and various amino acids and proteins needed by the human body. [0004] Seabuckthorn seeds are often used to extract oil to make seabuckt...
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IPC IPC(8): A61K31/165A61K45/06A61P9/00A61P35/00
CPCA61K31/165A61K45/06A61P9/00A61P35/00Y02A50/30
Inventor 王洪伦周文娜胡娜
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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