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Method for preparing ACE inhibitory peptide by enzymolysis of mulberry leaf protein

A technology for inhibiting peptides and mulberry leaves, which is applied in the field of enzymatic hydrolysis of mulberry leaf proteins to prepare ACE inhibitory peptides, which can solve problems such as side effects of patients

Active Publication Date: 2021-08-06
CHENGDE MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the ACE inhibitors commonly used in clinical practice are synthetic drugs, such as captopril, arapril, valsartan, etc., all of which have ACE inhibitory effect, but long-term use will cause side effects to patients

Method used

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  • Method for preparing ACE inhibitory peptide by enzymolysis of mulberry leaf protein
  • Method for preparing ACE inhibitory peptide by enzymolysis of mulberry leaf protein
  • Method for preparing ACE inhibitory peptide by enzymolysis of mulberry leaf protein

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for enzymatically hydrolyzing mulberry leaf protein to prepare ACE inhibitory peptides, comprising the following steps:

[0029] (1) Take fresh mulberry leaves and dry and pulverize them into mulberry leaf powder, add distilled water and mix evenly according to the material-to-liquid ratio of 1:20, and perform ultrasonic extraction to obtain a suspension;

[0030] (2) centrifuging the suspension to obtain a supernatant; adding HCl to adjust the pH to 3.5, and centrifuging again to obtain a precipitated protein;

[0031] (3) Resuspend the precipitated protein in distilled water, adjust the pH to 7.0, centrifuge again to obtain the precipitate, freeze-dry to obtain the mulberry leaf protein powder, and store it at -20°C for later use;

[0032] (4) Take mulberry leaf protein powder, add distilled water to make an enzymatic hydrolysis substrate, mix evenly in a water bath at a constant temperature of 55°C, adjust the pH to 6.5, then add an enzyme with a mass of 7.5...

Embodiment 2

[0043] A method for enzymatically hydrolyzing mulberry leaf protein to prepare ACE inhibitory peptides, comprising the following steps:

[0044] (1) Take fresh mulberry leaves and dry and pulverize them into mulberry leaf powder, add distilled water and mix evenly according to the material-to-liquid ratio of 1:20, and perform ultrasonic extraction to obtain a suspension;

[0045] (2) centrifuging the suspension to obtain a supernatant; adding HCl to adjust the pH to 3.5, and centrifuging again to obtain a precipitated protein;

[0046] (3) Resuspend the precipitated protein in distilled water, adjust the pH to 7.0, centrifuge again to obtain the precipitate, freeze-dry to obtain the mulberry leaf protein powder, and store it at -20°C for later use;

[0047] (4) Take mulberry leaf protein powder, add distilled water to prepare the substrate for enzymolysis, mix evenly in a water bath at a constant temperature of 45°C, adjust the pH to 7, then add enzyme with 7.5% of the substra...

Embodiment 3

[0058] A method for enzymatically hydrolyzing mulberry leaf protein to prepare ACE inhibitory peptides, comprising the following steps:

[0059] (1) Take fresh mulberry leaves and dry and pulverize them into mulberry leaf powder, add distilled water and mix evenly according to the material-to-liquid ratio of 1:20, and perform ultrasonic extraction to obtain a suspension;

[0060] (2) centrifuging the suspension to obtain a supernatant; adding HCl to adjust the pH to 3.5, and centrifuging again to obtain a precipitated protein;

[0061] (3) Resuspend the precipitated protein in distilled water, adjust the pH to 7.0, centrifuge again to obtain the precipitate, freeze-dry to obtain the mulberry leaf protein powder, and store it at -20°C for later use;

[0062] (4) Take mulberry leaf protein powder, add distilled water to make an enzymatic hydrolysis substrate, mix evenly in a water bath at a constant temperature of 37°C, adjust the pH to 2, then add an enzyme with a mass of 7.5% ...

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Abstract

The invention discloses a method for preparing ACE inhibitory peptide by enzymolysis of mulberry leaf protein, and belongs to the technical field of functional materials. The method comprises the following steps that fresh mulberry leaves are dried and crushed into mulberry leaf powder, and ultrasonic extraction and centrifugal separation are carried out to obtain precipitated protein; and enzymolysis, centrifugal separation and the like are carried out on the precipitated protein to obtain the finished product ACE inhibitory peptide. Under the enzymolysis technology, the hydrolysis degree of the mulberry leaf protein is 29.05%, and the ACE inhibition rate of enzymatic hydrolysate reaches 82.46%; and the preparation technology is simple, convenient to operate and low in cost and has wide market value.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a method for enzymolyzing mulberry leaf protein to prepare ACE inhibitory peptides. Background technique [0002] Angiotensin-converting enzyme (Angiotensin I-convening enzyme, ACE) is one of the main causes of elevated blood pressure. ACE can convert Angiotensin I (angiotensin I, Ang I) into Angiotensin II (angiotensin II, Ang II), and at the same time lead to an increase in blood pressure by degrading or inhibiting bradykinin, which has a hypotensive effect. At present, most of the ACE inhibitors commonly used in clinic are synthetic drugs, such as captopril, arapril, valsartan, etc., all of which have ACE inhibitory effect, but long-term use will cause side effects to patients. ACE inhibitory peptides, also known as antihypertensive peptides, are a class of polypeptides that can inhibit the activity of angiotensin-converting enzymes. Most of them are ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 贾漫丽范伟李娜王彬彬李季生杨雪
Owner CHENGDE MEDICAL UNIV
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